Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tetR(B and arsR overlap by 23 nucleotides arsR and BNILDI_09415 are separated by 70 nucleotides BNILDI_09415 and hmoA overlap by 8 nucleotides hmoA and gltS are separated by 443 nucleotides
BNILDI_09405: tetR(B - tetracycline resistance transcriptional repressor TetR(B), at 1,882,040 to 1,882,663
tetR(B
BNILDI_09410: arsR - transcriptional regulator, at 1,882,641 to 1,883,264
arsR
BNILDI_09415: BNILDI_09415 - amino acid-binding protein, at 1,883,335 to 1,883,721
_09415
BNILDI_09420: hmoA - antibiotic biosynthesis monooxygenase, at 1,883,714 to 1,884,034
hmoA
BNILDI_09425: gltS - sodium/glutamate symporter, at 1,884,478 to 1,885,683
gltS
Position (kb) 1883
1884 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1882.431 kb on + strand, within tetR(B at 1883.019 kb on + strand, within arsR at 1883.020 kb on - strand, within arsR at 1883.048 kb on + strand, within arsR at 1883.113 kb on - strand, within arsR at 1883.396 kb on - strand, within BNILDI_09415 at 1883.420 kb on + strand, within BNILDI_09415 at 1883.476 kb on - strand, within BNILDI_09415 at 1884.517 kb on - strand at 1884.530 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,882,431 + tetR(B BNILDI_09405 0.63 -2.9 1,883,019 + arsR BNILDI_09410 0.61 -1.0 1,883,020 - arsR BNILDI_09410 0.61 +0.2 1,883,048 + arsR BNILDI_09410 0.65 +1.3 1,883,113 - arsR BNILDI_09410 0.76 +0.3 1,883,396 - BNILDI_09415 0.16 +0.8 1,883,420 + BNILDI_09415 0.22 +0.3 1,883,476 - BNILDI_09415 0.36 +0.0 1,884,517 - -0.0 1,884,530 + -0.2
Or see this region's nucleotide sequence