Strain Fitness in Escherichia coli ECRC62 around BNILDI_09340

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyafD and ubiE are separated by 77 nucleotidesubiE and mltD are separated by 47 nucleotidesmltD and gloB are separated by 209 nucleotidesgloB and yafS are separated by 33 nucleotides BNILDI_09330: yafD - endonuclease/exonuclease/phosphatase family protein, at 1,870,148 to 1,870,927 yafD BNILDI_09335: ubiE - SAM-dependent methyltransferase, at 1,871,005 to 1,871,775 ubiE BNILDI_09340: mltD - murein transglycosylase D, at 1,871,823 to 1,873,043 mltD BNILDI_09345: gloB - hydroxyacylglutathione hydrolase, at 1,873,253 to 1,874,008 gloB BNILDI_09350: yafS - Uncharacterized protein YafS, at 1,874,042 to 1,874,764 yafS Position (kb) 1871 1872 1873 1874Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1870.926 kb on - strandat 1870.926 kb on - strandat 1870.930 kb on + strandat 1870.930 kb on + strandat 1870.931 kb on - strandat 1870.978 kb on - strandat 1871.033 kb on + strandat 1871.081 kb on + strandat 1871.082 kb on - strand, within ubiEat 1871.082 kb on - strand, within ubiEat 1871.148 kb on - strand, within ubiEat 1871.219 kb on - strand, within ubiEat 1871.219 kb on - strand, within ubiEat 1871.302 kb on + strand, within ubiEat 1871.447 kb on + strand, within ubiEat 1871.447 kb on + strand, within ubiEat 1871.448 kb on - strand, within ubiEat 1871.580 kb on + strand, within ubiEat 1871.595 kb on + strand, within ubiEat 1871.652 kb on - strand, within ubiEat 1871.654 kb on + strand, within ubiEat 1871.655 kb on - strand, within ubiEat 1871.655 kb on - strand, within ubiEat 1871.655 kb on - strand, within ubiEat 1871.763 kb on + strandat 1871.763 kb on + strandat 1871.763 kb on + strandat 1871.763 kb on + strandat 1871.763 kb on + strandat 1871.981 kb on + strand, within mltDat 1871.981 kb on + strand, within mltDat 1871.981 kb on + strand, within mltDat 1871.982 kb on - strand, within mltDat 1871.982 kb on - strand, within mltDat 1871.982 kb on - strand, within mltDat 1872.147 kb on + strand, within mltDat 1872.154 kb on + strand, within mltDat 1872.202 kb on - strand, within mltDat 1872.272 kb on - strand, within mltDat 1872.486 kb on - strand, within mltDat 1872.494 kb on - strand, within mltDat 1872.563 kb on + strand, within mltDat 1872.563 kb on + strand, within mltDat 1872.703 kb on + strand, within mltDat 1872.814 kb on - strand, within mltDat 1872.822 kb on + strand, within mltDat 1872.822 kb on + strand, within mltDat 1872.825 kb on + strand, within mltDat 1872.837 kb on - strand, within mltDat 1872.846 kb on - strand, within mltDat 1872.875 kb on - strand, within mltDat 1872.878 kb on + strand, within mltDat 1872.878 kb on + strand, within mltDat 1872.878 kb on + strand, within mltDat 1872.879 kb on - strand, within mltDat 1872.879 kb on - strand, within mltDat 1872.879 kb on - strand, within mltDat 1872.880 kb on + strand, within mltDat 1872.880 kb on + strand, within mltDat 1872.881 kb on - strand, within mltDat 1872.881 kb on - strand, within mltDat 1872.881 kb on - strand, within mltDat 1872.900 kb on + strand, within mltDat 1872.901 kb on - strand, within mltDat 1872.913 kb on + strand, within mltDat 1872.921 kb on - strand, within mltDat 1873.193 kb on + strandat 1873.262 kb on - strandat 1873.508 kb on - strand, within gloBat 1873.526 kb on + strand, within gloBat 1873.527 kb on - strand, within gloBat 1873.554 kb on - strand, within gloBat 1873.667 kb on - strand, within gloBat 1873.667 kb on - strand, within gloBat 1873.697 kb on + strand, within gloBat 1873.785 kb on + strand, within gloBat 1873.785 kb on + strand, within gloBat 1873.785 kb on + strand, within gloBat 1873.786 kb on - strand, within gloBat 1873.786 kb on - strand, within gloBat 1873.824 kb on - strand, within gloBat 1873.863 kb on - strand, within gloBat 1873.971 kb on + strandat 1873.971 kb on + strandat 1873.971 kb on + strandat 1873.971 kb on + strandat 1874.009 kb on - strandat 1874.014 kb on - strandat 1874.021 kb on - strandat 1874.021 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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1,870,926 - +0.1
1,870,926 - -0.3
1,870,930 + +0.2
1,870,930 + -0.4
1,870,931 - -1.2
1,870,978 - -3.4
1,871,033 + -0.3
1,871,081 + -3.5
1,871,082 - ubiE BNILDI_09335 0.10 +0.7
1,871,082 - ubiE BNILDI_09335 0.10 -0.5
1,871,148 - ubiE BNILDI_09335 0.19 +0.9
1,871,219 - ubiE BNILDI_09335 0.28 +0.4
1,871,219 - ubiE BNILDI_09335 0.28 -1.8
1,871,302 + ubiE BNILDI_09335 0.39 -0.8
1,871,447 + ubiE BNILDI_09335 0.57 -2.9
1,871,447 + ubiE BNILDI_09335 0.57 +0.3
1,871,448 - ubiE BNILDI_09335 0.57 -3.2
1,871,580 + ubiE BNILDI_09335 0.75 -1.2
1,871,595 + ubiE BNILDI_09335 0.77 +1.4
1,871,652 - ubiE BNILDI_09335 0.84 +0.4
1,871,654 + ubiE BNILDI_09335 0.84 +0.5
1,871,655 - ubiE BNILDI_09335 0.84 +0.1
1,871,655 - ubiE BNILDI_09335 0.84 +0.1
1,871,655 - ubiE BNILDI_09335 0.84 -0.3
1,871,763 + +0.9
1,871,763 + -0.6
1,871,763 + -0.7
1,871,763 + -0.7
1,871,763 + -2.4
1,871,981 + mltD BNILDI_09340 0.13 -1.1
1,871,981 + mltD BNILDI_09340 0.13 -0.2
1,871,981 + mltD BNILDI_09340 0.13 -0.4
1,871,982 - mltD BNILDI_09340 0.13 +0.6
1,871,982 - mltD BNILDI_09340 0.13 +0.1
1,871,982 - mltD BNILDI_09340 0.13 -0.9
1,872,147 + mltD BNILDI_09340 0.27 -1.3
1,872,154 + mltD BNILDI_09340 0.27 +0.1
1,872,202 - mltD BNILDI_09340 0.31 +1.0
1,872,272 - mltD BNILDI_09340 0.37 +0.5
1,872,486 - mltD BNILDI_09340 0.54 +0.8
1,872,494 - mltD BNILDI_09340 0.55 +0.5
1,872,563 + mltD BNILDI_09340 0.61 +0.9
1,872,563 + mltD BNILDI_09340 0.61 -0.5
1,872,703 + mltD BNILDI_09340 0.72 -3.2
1,872,814 - mltD BNILDI_09340 0.81 +1.5
1,872,822 + mltD BNILDI_09340 0.82 +0.5
1,872,822 + mltD BNILDI_09340 0.82 +0.2
1,872,825 + mltD BNILDI_09340 0.82 -0.3
1,872,837 - mltD BNILDI_09340 0.83 -0.4
1,872,846 - mltD BNILDI_09340 0.84 -2.1
1,872,875 - mltD BNILDI_09340 0.86 +0.7
1,872,878 + mltD BNILDI_09340 0.86 -0.9
1,872,878 + mltD BNILDI_09340 0.86 +0.6
1,872,878 + mltD BNILDI_09340 0.86 +2.3
1,872,879 - mltD BNILDI_09340 0.86 -0.7
1,872,879 - mltD BNILDI_09340 0.86 +1.2
1,872,879 - mltD BNILDI_09340 0.86 -0.4
1,872,880 + mltD BNILDI_09340 0.87 -2.2
1,872,880 + mltD BNILDI_09340 0.87 +0.3
1,872,881 - mltD BNILDI_09340 0.87 +0.3
1,872,881 - mltD BNILDI_09340 0.87 -1.6
1,872,881 - mltD BNILDI_09340 0.87 -1.2
1,872,900 + mltD BNILDI_09340 0.88 -0.1
1,872,901 - mltD BNILDI_09340 0.88 +0.4
1,872,913 + mltD BNILDI_09340 0.89 +0.6
1,872,921 - mltD BNILDI_09340 0.90 -0.2
1,873,193 + +0.3
1,873,262 - -3.2
1,873,508 - gloB BNILDI_09345 0.34 -0.7
1,873,526 + gloB BNILDI_09345 0.36 -3.0
1,873,527 - gloB BNILDI_09345 0.36 -1.3
1,873,554 - gloB BNILDI_09345 0.40 -0.2
1,873,667 - gloB BNILDI_09345 0.55 +1.0
1,873,667 - gloB BNILDI_09345 0.55 -0.9
1,873,697 + gloB BNILDI_09345 0.59 -0.4
1,873,785 + gloB BNILDI_09345 0.70 -0.7
1,873,785 + gloB BNILDI_09345 0.70 +2.2
1,873,785 + gloB BNILDI_09345 0.70 +0.3
1,873,786 - gloB BNILDI_09345 0.71 -0.5
1,873,786 - gloB BNILDI_09345 0.71 -0.2
1,873,824 - gloB BNILDI_09345 0.76 -0.8
1,873,863 - gloB BNILDI_09345 0.81 +0.2
1,873,971 + +0.4
1,873,971 + +0.2
1,873,971 + -1.1
1,873,971 + +1.0
1,874,009 - -1.2
1,874,014 - +0.1
1,874,021 - -1.6
1,874,021 - +0.2

Or see this region's nucleotide sequence