Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dapD and glnD are separated by 30 nucleotides glnD and map are separated by 61 nucleotides
BNILDI_09085: dapD - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 1,822,757 to 1,823,581
dapD
BNILDI_09090: glnD - bifunctional uridylyltransferase/uridylyl-removing protein GlnD, at 1,823,612 to 1,826,284
glnD
BNILDI_09095: map - type I methionyl aminopeptidase, at 1,826,346 to 1,827,140
map
Position (kb)
1823
1824
1825
1826
1827 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1822.620 kb on + strand at 1822.621 kb on - strand at 1822.639 kb on - strand at 1822.639 kb on - strand at 1822.704 kb on + strand at 1822.747 kb on - strand at 1822.747 kb on - strand at 1822.748 kb on + strand at 1822.748 kb on + strand at 1822.748 kb on + strand at 1822.748 kb on + strand at 1822.749 kb on - strand at 1822.749 kb on - strand at 1822.749 kb on - strand at 1822.753 kb on - strand at 1822.753 kb on - strand at 1822.758 kb on + strand at 1824.690 kb on - strand, within glnD at 1825.178 kb on - strand, within glnD at 1825.182 kb on - strand, within glnD at 1826.177 kb on + strand at 1827.178 kb on - strand at 1827.179 kb on + strand at 1827.179 kb on + strand at 1827.179 kb on + strand at 1827.179 kb on + strand at 1827.179 kb on + strand at 1827.179 kb on + strand at 1827.179 kb on + strand at 1827.179 kb on + strand at 1827.180 kb on - strand at 1827.180 kb on - strand at 1827.180 kb on - strand at 1827.184 kb on - strand at 1827.262 kb on + strand at 1827.267 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,822,620 + +0.0 1,822,621 - +0.6 1,822,639 - -0.5 1,822,639 - -0.7 1,822,704 + +0.1 1,822,747 - -2.7 1,822,747 - +0.9 1,822,748 + -2.5 1,822,748 + -1.1 1,822,748 + -2.7 1,822,748 + +0.0 1,822,749 - -2.6 1,822,749 - +0.3 1,822,749 - +2.1 1,822,753 - +0.7 1,822,753 - -0.1 1,822,758 + +0.2 1,824,690 - glnD BNILDI_09090 0.40 -1.5 1,825,178 - glnD BNILDI_09090 0.59 -0.3 1,825,182 - glnD BNILDI_09090 0.59 +1.4 1,826,177 + -1.6 1,827,178 - -0.9 1,827,179 + +0.6 1,827,179 + +0.0 1,827,179 + +0.3 1,827,179 + -0.3 1,827,179 + +0.9 1,827,179 + -1.4 1,827,179 + +2.7 1,827,179 + +0.9 1,827,180 - -2.8 1,827,180 - +0.4 1,827,180 - +0.4 1,827,184 - -0.1 1,827,262 + -2.8 1,827,267 + +0.5
Or see this region's nucleotide sequence