Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt robA and creA are separated by 210 nucleotides creA and creB are separated by 12 nucleotides creB and creC overlap by 1 nucleotides
BNILDI_08120: robA - MDR efflux pump AcrAB transcriptional activator RobA, at 1,620,244 to 1,621,113
robA
BNILDI_08125: creA - protein CreA, at 1,621,324 to 1,621,797
creA
BNILDI_08130: creB - two-component system response regulator CreB, at 1,621,810 to 1,622,499
creB
BNILDI_08135: creC - two-component system sensor histidine kinase CreC, at 1,622,499 to 1,623,923
creC
Position (kb)
1621
1622 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1620.324 kb on - strand at 1620.384 kb on - strand, within robA at 1620.396 kb on + strand, within robA at 1620.428 kb on + strand, within robA at 1620.429 kb on - strand, within robA at 1620.444 kb on - strand, within robA at 1620.481 kb on + strand, within robA at 1620.485 kb on + strand, within robA at 1620.485 kb on + strand, within robA at 1620.486 kb on - strand, within robA at 1620.486 kb on - strand, within robA at 1620.521 kb on + strand, within robA at 1620.522 kb on - strand, within robA at 1620.522 kb on - strand, within robA at 1620.522 kb on - strand, within robA at 1620.527 kb on - strand, within robA at 1620.527 kb on - strand, within robA at 1620.680 kb on + strand, within robA at 1620.680 kb on + strand, within robA at 1620.680 kb on + strand, within robA at 1620.681 kb on - strand, within robA at 1620.681 kb on - strand, within robA at 1620.681 kb on - strand, within robA at 1620.709 kb on - strand, within robA at 1620.916 kb on + strand, within robA at 1620.954 kb on + strand, within robA at 1620.955 kb on - strand, within robA at 1620.988 kb on - strand, within robA at 1621.016 kb on + strand, within robA at 1621.119 kb on - strand at 1621.157 kb on - strand at 1621.167 kb on + strand at 1621.214 kb on + strand at 1621.215 kb on - strand at 1621.222 kb on - strand at 1621.272 kb on + strand at 1621.290 kb on - strand at 1621.290 kb on - strand at 1621.416 kb on + strand, within creA at 1621.518 kb on + strand, within creA at 1621.518 kb on + strand, within creA at 1621.605 kb on + strand, within creA at 1621.631 kb on + strand, within creA at 1621.632 kb on - strand, within creA at 1621.632 kb on - strand, within creA at 1621.635 kb on - strand, within creA at 1621.662 kb on + strand, within creA at 1621.796 kb on - strand at 1621.809 kb on + strand at 1621.809 kb on + strand at 1621.810 kb on - strand at 1621.810 kb on - strand at 1621.832 kb on + strand at 1621.870 kb on + strand at 1621.870 kb on + strand at 1621.871 kb on - strand at 1622.015 kb on + strand, within creB at 1622.110 kb on + strand, within creB at 1622.110 kb on + strand, within creB at 1622.110 kb on + strand, within creB at 1622.110 kb on + strand, within creB at 1622.209 kb on - strand, within creB at 1622.209 kb on - strand, within creB at 1622.345 kb on + strand, within creB at 1622.345 kb on + strand, within creB at 1622.346 kb on - strand, within creB at 1622.346 kb on - strand, within creB at 1622.380 kb on + strand, within creB at 1622.406 kb on + strand, within creB at 1622.406 kb on + strand, within creB at 1622.407 kb on - strand, within creB at 1622.457 kb on + strand at 1622.498 kb on - strand at 1622.712 kb on - strand, within creC at 1622.775 kb on + strand, within creC at 1622.775 kb on + strand, within creC at 1622.776 kb on - strand, within creC at 1622.776 kb on - strand, within creC at 1622.784 kb on + strand, within creC at 1622.785 kb on - strand, within creC at 1622.785 kb on - strand, within creC at 1622.786 kb on + strand, within creC at 1622.786 kb on + strand, within creC at 1622.787 kb on - strand, within creC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,620,324 - +0.2 1,620,384 - robA BNILDI_08120 0.16 +0.3 1,620,396 + robA BNILDI_08120 0.17 +2.0 1,620,428 + robA BNILDI_08120 0.21 -0.2 1,620,429 - robA BNILDI_08120 0.21 +0.3 1,620,444 - robA BNILDI_08120 0.23 -0.2 1,620,481 + robA BNILDI_08120 0.27 -0.0 1,620,485 + robA BNILDI_08120 0.28 -2.2 1,620,485 + robA BNILDI_08120 0.28 -0.7 1,620,486 - robA BNILDI_08120 0.28 -0.1 1,620,486 - robA BNILDI_08120 0.28 -0.5 1,620,521 + robA BNILDI_08120 0.32 +0.4 1,620,522 - robA BNILDI_08120 0.32 -0.4 1,620,522 - robA BNILDI_08120 0.32 -0.8 1,620,522 - robA BNILDI_08120 0.32 -0.7 1,620,527 - robA BNILDI_08120 0.33 -0.3 1,620,527 - robA BNILDI_08120 0.33 -0.0 1,620,680 + robA BNILDI_08120 0.50 -1.0 1,620,680 + robA BNILDI_08120 0.50 -0.5 1,620,680 + robA BNILDI_08120 0.50 -2.3 1,620,681 - robA BNILDI_08120 0.50 -0.5 1,620,681 - robA BNILDI_08120 0.50 +3.8 1,620,681 - robA BNILDI_08120 0.50 +1.0 1,620,709 - robA BNILDI_08120 0.53 +0.7 1,620,916 + robA BNILDI_08120 0.77 +0.4 1,620,954 + robA BNILDI_08120 0.82 +0.9 1,620,955 - robA BNILDI_08120 0.82 -2.8 1,620,988 - robA BNILDI_08120 0.86 -0.1 1,621,016 + robA BNILDI_08120 0.89 +0.4 1,621,119 - +0.5 1,621,157 - +0.1 1,621,167 + -1.2 1,621,214 + -0.5 1,621,215 - +0.2 1,621,222 - +0.6 1,621,272 + +0.4 1,621,290 - +3.6 1,621,290 - +0.4 1,621,416 + creA BNILDI_08125 0.19 +2.0 1,621,518 + creA BNILDI_08125 0.41 +0.3 1,621,518 + creA BNILDI_08125 0.41 -0.7 1,621,605 + creA BNILDI_08125 0.59 +0.4 1,621,631 + creA BNILDI_08125 0.65 +0.4 1,621,632 - creA BNILDI_08125 0.65 -0.4 1,621,632 - creA BNILDI_08125 0.65 +0.3 1,621,635 - creA BNILDI_08125 0.66 +0.8 1,621,662 + creA BNILDI_08125 0.71 -1.8 1,621,796 - -0.2 1,621,809 + -0.1 1,621,809 + +1.2 1,621,810 - +0.6 1,621,810 - -2.3 1,621,832 + +0.9 1,621,870 + -2.4 1,621,870 + -0.7 1,621,871 - -0.5 1,622,015 + creB BNILDI_08130 0.30 -2.8 1,622,110 + creB BNILDI_08130 0.43 -0.4 1,622,110 + creB BNILDI_08130 0.43 -1.3 1,622,110 + creB BNILDI_08130 0.43 +0.8 1,622,110 + creB BNILDI_08130 0.43 +0.1 1,622,209 - creB BNILDI_08130 0.58 -1.3 1,622,209 - creB BNILDI_08130 0.58 +0.2 1,622,345 + creB BNILDI_08130 0.78 -3.2 1,622,345 + creB BNILDI_08130 0.78 -1.0 1,622,346 - creB BNILDI_08130 0.78 +1.1 1,622,346 - creB BNILDI_08130 0.78 -3.8 1,622,380 + creB BNILDI_08130 0.83 -2.8 1,622,406 + creB BNILDI_08130 0.86 +0.7 1,622,406 + creB BNILDI_08130 0.86 -0.5 1,622,407 - creB BNILDI_08130 0.87 +0.8 1,622,457 + -3.1 1,622,498 - -0.1 1,622,712 - creC BNILDI_08135 0.15 +0.1 1,622,775 + creC BNILDI_08135 0.19 -2.8 1,622,775 + creC BNILDI_08135 0.19 +0.8 1,622,776 - creC BNILDI_08135 0.19 -1.2 1,622,776 - creC BNILDI_08135 0.19 +1.4 1,622,784 + creC BNILDI_08135 0.20 -2.6 1,622,785 - creC BNILDI_08135 0.20 +0.3 1,622,785 - creC BNILDI_08135 0.20 +0.1 1,622,786 + creC BNILDI_08135 0.20 +0.0 1,622,786 + creC BNILDI_08135 0.20 -3.8 1,622,787 - creC BNILDI_08135 0.20 +0.2
Or see this region's nucleotide sequence