Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dapA and BNILDI_07515 are separated by 16 nucleotides BNILDI_07515 and BNILDI_07520 are separated by 77 nucleotides
BNILDI_07510: dapA - dihydrodipicolinate synthase family protein, at 1,497,517 to 1,498,416
dapA
BNILDI_07515: BNILDI_07515 - galactonate dehydratase, at 1,498,433 to 1,499,950
_07515
BNILDI_07520: BNILDI_07520 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 1,500,028 to 1,501,041
_07520
Position (kb)
1498
1499
1500 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1497.516 kb on + strand at 1497.517 kb on - strand at 1497.517 kb on - strand at 1497.537 kb on + strand at 1497.538 kb on - strand at 1497.555 kb on + strand at 1497.555 kb on + strand at 1497.556 kb on - strand at 1497.581 kb on + strand at 1497.581 kb on + strand at 1497.582 kb on - strand at 1497.586 kb on - strand at 1497.701 kb on + strand, within dapA at 1497.702 kb on - strand, within dapA at 1497.716 kb on + strand, within dapA at 1497.717 kb on - strand, within dapA at 1497.725 kb on + strand, within dapA at 1497.725 kb on + strand, within dapA at 1497.725 kb on + strand, within dapA at 1497.726 kb on - strand, within dapA at 1497.726 kb on - strand, within dapA at 1497.788 kb on + strand, within dapA at 1497.924 kb on + strand, within dapA at 1498.086 kb on - strand, within dapA at 1498.105 kb on + strand, within dapA at 1498.105 kb on + strand, within dapA at 1498.105 kb on + strand, within dapA at 1498.106 kb on - strand, within dapA at 1498.106 kb on - strand, within dapA at 1498.186 kb on - strand, within dapA at 1498.333 kb on - strand at 1498.333 kb on - strand at 1498.528 kb on + strand at 1498.657 kb on + strand, within BNILDI_07515 at 1498.658 kb on - strand, within BNILDI_07515 at 1498.669 kb on + strand, within BNILDI_07515 at 1498.714 kb on - strand, within BNILDI_07515 at 1498.757 kb on - strand, within BNILDI_07515 at 1498.800 kb on - strand, within BNILDI_07515 at 1498.800 kb on - strand, within BNILDI_07515 at 1498.801 kb on + strand, within BNILDI_07515 at 1498.802 kb on - strand, within BNILDI_07515 at 1498.821 kb on + strand, within BNILDI_07515 at 1498.839 kb on + strand, within BNILDI_07515 at 1498.839 kb on + strand, within BNILDI_07515 at 1498.840 kb on - strand, within BNILDI_07515 at 1498.904 kb on - strand, within BNILDI_07515 at 1498.904 kb on - strand, within BNILDI_07515 at 1498.904 kb on - strand, within BNILDI_07515 at 1498.904 kb on - strand, within BNILDI_07515 at 1498.904 kb on - strand, within BNILDI_07515 at 1499.079 kb on + strand, within BNILDI_07515 at 1499.080 kb on - strand, within BNILDI_07515 at 1499.094 kb on + strand, within BNILDI_07515 at 1499.102 kb on - strand, within BNILDI_07515 at 1499.111 kb on - strand, within BNILDI_07515 at 1499.134 kb on + strand, within BNILDI_07515 at 1499.254 kb on + strand, within BNILDI_07515 at 1499.254 kb on + strand, within BNILDI_07515 at 1499.258 kb on + strand, within BNILDI_07515 at 1499.259 kb on - strand, within BNILDI_07515 at 1499.390 kb on - strand, within BNILDI_07515 at 1499.426 kb on - strand, within BNILDI_07515 at 1499.426 kb on - strand, within BNILDI_07515 at 1499.426 kb on - strand, within BNILDI_07515 at 1499.452 kb on + strand, within BNILDI_07515 at 1499.452 kb on + strand, within BNILDI_07515 at 1499.489 kb on - strand, within BNILDI_07515 at 1499.489 kb on - strand, within BNILDI_07515 at 1499.492 kb on - strand, within BNILDI_07515 at 1499.539 kb on - strand, within BNILDI_07515 at 1499.579 kb on + strand, within BNILDI_07515 at 1499.842 kb on + strand at 1499.842 kb on + strand at 1499.948 kb on + strand at 1499.948 kb on + strand at 1499.956 kb on - strand at 1500.017 kb on - strand at 1500.114 kb on + strand at 1500.123 kb on + strand at 1500.123 kb on + strand at 1500.240 kb on + strand, within BNILDI_07520 at 1500.352 kb on - strand, within BNILDI_07520 at 1500.359 kb on + strand, within BNILDI_07520 at 1500.452 kb on - strand, within BNILDI_07520 at 1500.558 kb on + strand, within BNILDI_07520 at 1500.574 kb on - strand, within BNILDI_07520 at 1500.605 kb on + strand, within BNILDI_07520 at 1500.620 kb on + strand, within BNILDI_07520 at 1500.620 kb on + strand, within BNILDI_07520 at 1500.621 kb on - strand, within BNILDI_07520 at 1500.621 kb on - strand, within BNILDI_07520 at 1500.625 kb on - strand, within BNILDI_07520 at 1500.666 kb on + strand, within BNILDI_07520 at 1500.666 kb on + strand, within BNILDI_07520 at 1500.700 kb on - strand, within BNILDI_07520 at 1500.759 kb on + strand, within BNILDI_07520 at 1500.760 kb on - strand, within BNILDI_07520 at 1500.763 kb on + strand, within BNILDI_07520 at 1500.804 kb on + strand, within BNILDI_07520 at 1500.805 kb on - strand, within BNILDI_07520 at 1500.814 kb on + strand, within BNILDI_07520 at 1500.814 kb on + strand, within BNILDI_07520 at 1500.918 kb on + strand, within BNILDI_07520
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,497,516 + -0.3 1,497,517 - +0.2 1,497,517 - +0.2 1,497,537 + +0.7 1,497,538 - +0.1 1,497,555 + -0.1 1,497,555 + -0.2 1,497,556 - +0.6 1,497,581 + -0.9 1,497,581 + -0.8 1,497,582 - +0.4 1,497,586 - -1.1 1,497,701 + dapA BNILDI_07510 0.20 -0.1 1,497,702 - dapA BNILDI_07510 0.21 +0.3 1,497,716 + dapA BNILDI_07510 0.22 -0.4 1,497,717 - dapA BNILDI_07510 0.22 -0.2 1,497,725 + dapA BNILDI_07510 0.23 +0.3 1,497,725 + dapA BNILDI_07510 0.23 +0.1 1,497,725 + dapA BNILDI_07510 0.23 -0.2 1,497,726 - dapA BNILDI_07510 0.23 -0.3 1,497,726 - dapA BNILDI_07510 0.23 +0.3 1,497,788 + dapA BNILDI_07510 0.30 -0.1 1,497,924 + dapA BNILDI_07510 0.45 -0.2 1,498,086 - dapA BNILDI_07510 0.63 +0.9 1,498,105 + dapA BNILDI_07510 0.65 +0.8 1,498,105 + dapA BNILDI_07510 0.65 +1.2 1,498,105 + dapA BNILDI_07510 0.65 -0.6 1,498,106 - dapA BNILDI_07510 0.65 -1.6 1,498,106 - dapA BNILDI_07510 0.65 -0.2 1,498,186 - dapA BNILDI_07510 0.74 +0.3 1,498,333 - +0.4 1,498,333 - +1.4 1,498,528 + +0.6 1,498,657 + BNILDI_07515 0.15 +0.5 1,498,658 - BNILDI_07515 0.15 -2.8 1,498,669 + BNILDI_07515 0.16 +0.1 1,498,714 - BNILDI_07515 0.19 +0.5 1,498,757 - BNILDI_07515 0.21 +0.1 1,498,800 - BNILDI_07515 0.24 +1.3 1,498,800 - BNILDI_07515 0.24 +1.2 1,498,801 + BNILDI_07515 0.24 +1.0 1,498,802 - BNILDI_07515 0.24 +0.7 1,498,821 + BNILDI_07515 0.26 -2.2 1,498,839 + BNILDI_07515 0.27 +1.5 1,498,839 + BNILDI_07515 0.27 -2.4 1,498,840 - BNILDI_07515 0.27 +0.6 1,498,904 - BNILDI_07515 0.31 -2.2 1,498,904 - BNILDI_07515 0.31 -1.7 1,498,904 - BNILDI_07515 0.31 +2.1 1,498,904 - BNILDI_07515 0.31 -0.1 1,498,904 - BNILDI_07515 0.31 -1.8 1,499,079 + BNILDI_07515 0.43 +1.7 1,499,080 - BNILDI_07515 0.43 -0.8 1,499,094 + BNILDI_07515 0.44 -0.3 1,499,102 - BNILDI_07515 0.44 -0.9 1,499,111 - BNILDI_07515 0.45 +0.8 1,499,134 + BNILDI_07515 0.46 +1.8 1,499,254 + BNILDI_07515 0.54 +0.4 1,499,254 + BNILDI_07515 0.54 -1.6 1,499,258 + BNILDI_07515 0.54 +0.3 1,499,259 - BNILDI_07515 0.54 -0.6 1,499,390 - BNILDI_07515 0.63 -1.0 1,499,426 - BNILDI_07515 0.65 +1.4 1,499,426 - BNILDI_07515 0.65 -0.9 1,499,426 - BNILDI_07515 0.65 +1.0 1,499,452 + BNILDI_07515 0.67 -1.1 1,499,452 + BNILDI_07515 0.67 +0.3 1,499,489 - BNILDI_07515 0.70 +0.1 1,499,489 - BNILDI_07515 0.70 +0.9 1,499,492 - BNILDI_07515 0.70 +2.1 1,499,539 - BNILDI_07515 0.73 -2.0 1,499,579 + BNILDI_07515 0.75 +0.9 1,499,842 + +0.6 1,499,842 + -0.1 1,499,948 + +0.7 1,499,948 + +1.9 1,499,956 - -0.3 1,500,017 - -0.7 1,500,114 + -0.9 1,500,123 + -0.3 1,500,123 + -1.0 1,500,240 + BNILDI_07520 0.21 -0.2 1,500,352 - BNILDI_07520 0.32 -0.9 1,500,359 + BNILDI_07520 0.33 -0.4 1,500,452 - BNILDI_07520 0.42 -0.7 1,500,558 + BNILDI_07520 0.52 +0.2 1,500,574 - BNILDI_07520 0.54 -2.2 1,500,605 + BNILDI_07520 0.57 +1.0 1,500,620 + BNILDI_07520 0.58 -0.9 1,500,620 + BNILDI_07520 0.58 -0.2 1,500,621 - BNILDI_07520 0.58 -0.3 1,500,621 - BNILDI_07520 0.58 -1.5 1,500,625 - BNILDI_07520 0.59 -0.1 1,500,666 + BNILDI_07520 0.63 +0.0 1,500,666 + BNILDI_07520 0.63 +0.2 1,500,700 - BNILDI_07520 0.66 -0.2 1,500,759 + BNILDI_07520 0.72 -0.2 1,500,760 - BNILDI_07520 0.72 +0.4 1,500,763 + BNILDI_07520 0.72 -0.8 1,500,804 + BNILDI_07520 0.77 +0.3 1,500,805 - BNILDI_07520 0.77 +0.8 1,500,814 + BNILDI_07520 0.78 +0.3 1,500,814 + BNILDI_07520 0.78 +1.9 1,500,918 + BNILDI_07520 0.88 +2.0
Or see this region's nucleotide sequence