Strain Fitness in Escherichia coli ECRC62 around BNILDI_06590

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntphnH and phnG overlap by 4 nucleotidesphnG and phnF are separated by 0 nucleotidesphnF and BNILDI_06595 are separated by 20 nucleotidesBNILDI_06595 and phnD are separated by 61 nucleotides BNILDI_06580: phnH - phosphonate C-P lyase system protein PhnH, at 1,315,234 to 1,315,818 phnH BNILDI_06585: phnG - phosphonate C-P lyase system protein PhnG, at 1,315,815 to 1,316,267 phnG BNILDI_06590: phnF - phosphonate metabolism transcriptional regulator PhnF, at 1,316,268 to 1,316,993 phnF BNILDI_06595: BNILDI_06595 - Phosphonate ABC transporter, permease protein PhnE, at 1,317,014 to 1,317,793 _06595 BNILDI_06600: phnD - phosphonate ABC transporter substrate-binding protein, at 1,317,855 to 1,318,871 phnD Position (kb) 1316 1317Strain fitness (log2 ratio) -1 0 1 2 3at 1315.444 kb on + strand, within phnHat 1315.578 kb on + strand, within phnHat 1315.797 kb on + strandat 1315.798 kb on - strandat 1315.798 kb on - strandat 1315.935 kb on + strand, within phnGat 1316.027 kb on - strand, within phnGat 1316.384 kb on + strand, within phnFat 1316.548 kb on + strand, within phnFat 1316.560 kb on - strand, within phnFat 1316.560 kb on - strand, within phnFat 1316.800 kb on - strand, within phnFat 1316.894 kb on - strand, within phnFat 1317.020 kb on - strandat 1317.020 kb on - strandat 1317.076 kb on + strandat 1317.213 kb on + strand, within BNILDI_06595at 1317.234 kb on - strand, within BNILDI_06595at 1317.483 kb on + strand, within BNILDI_06595at 1317.600 kb on + strand, within BNILDI_06595at 1317.601 kb on - strand, within BNILDI_06595at 1317.760 kb on + strandat 1317.761 kb on - strandat 1317.808 kb on - strandat 1317.857 kb on - strandat 1317.870 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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1,315,444 + phnH BNILDI_06580 0.36 -0.7
1,315,578 + phnH BNILDI_06580 0.59 -0.5
1,315,797 + +1.0
1,315,798 - +0.9
1,315,798 - -0.5
1,315,935 + phnG BNILDI_06585 0.26 +0.1
1,316,027 - phnG BNILDI_06585 0.47 -0.3
1,316,384 + phnF BNILDI_06590 0.16 -0.2
1,316,548 + phnF BNILDI_06590 0.39 -0.1
1,316,560 - phnF BNILDI_06590 0.40 +2.8
1,316,560 - phnF BNILDI_06590 0.40 -1.4
1,316,800 - phnF BNILDI_06590 0.73 +1.0
1,316,894 - phnF BNILDI_06590 0.86 +2.0
1,317,020 - -0.7
1,317,020 - +0.5
1,317,076 + +0.5
1,317,213 + BNILDI_06595 0.26 +0.6
1,317,234 - BNILDI_06595 0.28 +0.9
1,317,483 + BNILDI_06595 0.60 -0.1
1,317,600 + BNILDI_06595 0.75 +0.8
1,317,601 - BNILDI_06595 0.75 +0.2
1,317,760 + -0.0
1,317,761 - +0.1
1,317,808 - -0.5
1,317,857 - -0.0
1,317,870 - -0.6

Or see this region's nucleotide sequence