Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nrfA and nrfB are separated by 44 nucleotides nrfB and nrfC overlap by 4 nucleotides nrfC and nrfD overlap by 4 nucleotides
BNILDI_06455: nrfA - ammonia-forming nitrite reductase cytochrome c552 subunit, at 1,289,465 to 1,290,901
nrfA
BNILDI_06460: nrfB - cytochrome c nitrite reductase pentaheme subunit, at 1,290,946 to 1,291,512
nrfB
BNILDI_06465: nrfC - cytochrome c nitrite reductase Fe-S protein, at 1,291,509 to 1,292,180
nrfC
BNILDI_06470: nrfD - cytochrome c nitrite reductase subunit NrfD, at 1,292,177 to 1,293,133
nrfD
Position (kb)
1291
1292
1293 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1290.637 kb on + strand, within nrfA at 1290.638 kb on - strand, within nrfA at 1290.877 kb on + strand at 1290.878 kb on - strand at 1290.945 kb on + strand at 1290.945 kb on + strand at 1290.946 kb on - strand at 1290.946 kb on - strand at 1290.953 kb on + strand at 1290.953 kb on + strand at 1290.954 kb on - strand at 1290.969 kb on - strand at 1290.969 kb on - strand at 1291.105 kb on - strand, within nrfB at 1291.122 kb on + strand, within nrfB at 1291.122 kb on + strand, within nrfB at 1291.122 kb on + strand, within nrfB at 1291.123 kb on - strand, within nrfB at 1291.123 kb on - strand, within nrfB at 1291.123 kb on - strand, within nrfB at 1291.123 kb on - strand, within nrfB at 1291.123 kb on - strand, within nrfB at 1291.123 kb on - strand, within nrfB at 1291.158 kb on + strand, within nrfB at 1291.255 kb on + strand, within nrfB at 1291.255 kb on + strand, within nrfB at 1291.256 kb on - strand, within nrfB at 1291.284 kb on + strand, within nrfB at 1291.285 kb on - strand, within nrfB at 1291.407 kb on - strand, within nrfB at 1291.665 kb on - strand, within nrfC at 1291.761 kb on + strand, within nrfC at 1291.775 kb on + strand, within nrfC at 1291.869 kb on - strand, within nrfC at 1292.099 kb on + strand, within nrfC at 1292.099 kb on + strand, within nrfC at 1292.099 kb on + strand, within nrfC at 1292.100 kb on - strand, within nrfC at 1292.100 kb on - strand, within nrfC at 1292.156 kb on + strand at 1292.438 kb on + strand, within nrfD at 1292.486 kb on + strand, within nrfD at 1292.487 kb on - strand, within nrfD at 1292.661 kb on - strand, within nrfD at 1292.661 kb on - strand, within nrfD at 1292.661 kb on - strand, within nrfD at 1292.937 kb on + strand, within nrfD at 1292.937 kb on + strand, within nrfD at 1292.937 kb on + strand, within nrfD at 1292.937 kb on + strand, within nrfD at 1292.938 kb on - strand, within nrfD at 1293.131 kb on + strand at 1293.160 kb on + strand at 1293.160 kb on + strand at 1293.161 kb on - strand at 1293.161 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,290,637 + nrfA BNILDI_06455 0.82 -3.0 1,290,638 - nrfA BNILDI_06455 0.82 +1.0 1,290,877 + +2.3 1,290,878 - -2.1 1,290,945 + -2.7 1,290,945 + -1.0 1,290,946 - +0.1 1,290,946 - -0.6 1,290,953 + +0.3 1,290,953 + +0.3 1,290,954 - +0.4 1,290,969 - -3.2 1,290,969 - +0.2 1,291,105 - nrfB BNILDI_06460 0.28 +0.8 1,291,122 + nrfB BNILDI_06460 0.31 +0.0 1,291,122 + nrfB BNILDI_06460 0.31 +1.0 1,291,122 + nrfB BNILDI_06460 0.31 -0.2 1,291,123 - nrfB BNILDI_06460 0.31 -0.9 1,291,123 - nrfB BNILDI_06460 0.31 -1.2 1,291,123 - nrfB BNILDI_06460 0.31 -0.7 1,291,123 - nrfB BNILDI_06460 0.31 -3.9 1,291,123 - nrfB BNILDI_06460 0.31 +0.4 1,291,123 - nrfB BNILDI_06460 0.31 -0.2 1,291,158 + nrfB BNILDI_06460 0.37 -2.9 1,291,255 + nrfB BNILDI_06460 0.54 -1.3 1,291,255 + nrfB BNILDI_06460 0.54 -0.2 1,291,256 - nrfB BNILDI_06460 0.55 -0.2 1,291,284 + nrfB BNILDI_06460 0.60 -1.7 1,291,285 - nrfB BNILDI_06460 0.60 +1.0 1,291,407 - nrfB BNILDI_06460 0.81 +1.3 1,291,665 - nrfC BNILDI_06465 0.23 +0.6 1,291,761 + nrfC BNILDI_06465 0.38 +0.9 1,291,775 + nrfC BNILDI_06465 0.40 +0.1 1,291,869 - nrfC BNILDI_06465 0.54 -3.0 1,292,099 + nrfC BNILDI_06465 0.88 -0.6 1,292,099 + nrfC BNILDI_06465 0.88 +1.0 1,292,099 + nrfC BNILDI_06465 0.88 +0.4 1,292,100 - nrfC BNILDI_06465 0.88 -0.0 1,292,100 - nrfC BNILDI_06465 0.88 -0.5 1,292,156 + -1.0 1,292,438 + nrfD BNILDI_06470 0.27 +0.7 1,292,486 + nrfD BNILDI_06470 0.32 -1.5 1,292,487 - nrfD BNILDI_06470 0.32 -1.1 1,292,661 - nrfD BNILDI_06470 0.51 +0.4 1,292,661 - nrfD BNILDI_06470 0.51 +0.2 1,292,661 - nrfD BNILDI_06470 0.51 +0.5 1,292,937 + nrfD BNILDI_06470 0.79 +1.3 1,292,937 + nrfD BNILDI_06470 0.79 -1.0 1,292,937 + nrfD BNILDI_06470 0.79 +0.4 1,292,937 + nrfD BNILDI_06470 0.79 -0.9 1,292,938 - nrfD BNILDI_06470 0.80 +0.2 1,293,131 + -1.9 1,293,160 + -0.7 1,293,160 + +0.2 1,293,161 - +0.1 1,293,161 - +0.5
Or see this region's nucleotide sequence