Strain Fitness in Escherichia coli ECRC62 around BNILDI_05995

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthiH and thiG overlap by 4 nucleotidesthiG and thiF are separated by 1 nucleotidesthiF and thiF overlap by 17 nucleotidesthiF and thiE overlap by 8 nucleotidesthiE and thiC overlap by 1 nucleotides BNILDI_05980: thiH - 2-iminoacetate synthase ThiH, at 1,190,370 to 1,191,503 thiH BNILDI_05985: thiG - thiazole synthase, at 1,191,500 to 1,192,270 thiG BNILDI_05990: thiF - thiazole biosynthesis adenylyltransferase ThiF, at 1,192,272 to 1,192,472 thiF BNILDI_05995: thiF - thiazole biosynthesis adenylyltransferase ThiF, at 1,192,456 to 1,193,211 thiF BNILDI_06000: thiE - thiamine phosphate synthase, at 1,193,204 to 1,193,839 thiE BNILDI_06005: thiC - phosphomethylpyrimidine synthase ThiC, at 1,193,839 to 1,195,734 thiC Position (kb) 1192 1193 1194Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 1191.763 kb on + strand, within thiGat 1191.763 kb on + strand, within thiGat 1191.859 kb on + strand, within thiGat 1191.885 kb on + strand, within thiGat 1192.066 kb on + strand, within thiGat 1192.067 kb on - strand, within thiGat 1192.262 kb on - strandat 1192.325 kb on + strand, within thiFat 1192.326 kb on - strand, within thiFat 1192.365 kb on - strand, within thiFat 1192.411 kb on - strand, within thiFat 1192.411 kb on - strand, within thiFat 1192.576 kb on + strand, within thiFat 1192.579 kb on - strand, within thiFat 1192.588 kb on + strand, within thiFat 1192.589 kb on - strand, within thiFat 1192.594 kb on - strand, within thiFat 1192.704 kb on - strand, within thiFat 1192.774 kb on - strand, within thiFat 1192.807 kb on - strand, within thiFat 1192.827 kb on + strand, within thiFat 1192.828 kb on - strand, within thiFat 1192.903 kb on + strand, within thiFat 1192.904 kb on - strand, within thiFat 1192.904 kb on - strand, within thiFat 1192.904 kb on - strand, within thiFat 1192.904 kb on - strand, within thiFat 1193.075 kb on + strand, within thiFat 1193.075 kb on + strand, within thiFat 1193.075 kb on + strand, within thiFat 1193.076 kb on - strand, within thiFat 1193.184 kb on + strandat 1193.464 kb on + strand, within thiEat 1193.465 kb on - strand, within thiEat 1193.466 kb on + strand, within thiEat 1193.466 kb on + strand, within thiEat 1193.466 kb on + strand, within thiEat 1193.467 kb on - strand, within thiEat 1193.467 kb on - strand, within thiEat 1193.467 kb on - strand, within thiEat 1193.467 kb on - strand, within thiEat 1193.598 kb on + strand, within thiEat 1193.599 kb on - strand, within thiEat 1193.599 kb on - strand, within thiEat 1193.638 kb on + strand, within thiEat 1193.727 kb on - strand, within thiEat 1193.737 kb on + strand, within thiEat 1193.738 kb on - strand, within thiEat 1193.738 kb on - strand, within thiEat 1193.741 kb on + strand, within thiEat 1193.742 kb on - strand, within thiEat 1193.774 kb on + strand, within thiEat 1193.775 kb on - strand, within thiEat 1193.775 kb on - strand, within thiEat 1193.835 kb on + strandat 1193.836 kb on - strandat 1193.920 kb on - strandat 1193.920 kb on - strandat 1193.920 kb on - strandat 1194.075 kb on + strand, within thiCat 1194.183 kb on + strand, within thiCat 1194.183 kb on + strand, within thiCat 1194.185 kb on + strand, within thiCat 1194.185 kb on + strand, within thiCat 1194.185 kb on + strand, within thiCat 1194.186 kb on - strand, within thiCat 1194.193 kb on - strand, within thiCat 1194.205 kb on - strand, within thiC

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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1,191,763 + thiG BNILDI_05985 0.34 -3.2
1,191,763 + thiG BNILDI_05985 0.34 -0.3
1,191,859 + thiG BNILDI_05985 0.47 +0.7
1,191,885 + thiG BNILDI_05985 0.50 +0.7
1,192,066 + thiG BNILDI_05985 0.73 +0.2
1,192,067 - thiG BNILDI_05985 0.74 -1.1
1,192,262 - -0.9
1,192,325 + thiF BNILDI_05990 0.26 -0.1
1,192,326 - thiF BNILDI_05990 0.27 -0.9
1,192,365 - thiF BNILDI_05990 0.46 -1.8
1,192,411 - thiF BNILDI_05990 0.69 -0.6
1,192,411 - thiF BNILDI_05990 0.69 +0.3
1,192,576 + thiF BNILDI_05995 0.16 -2.0
1,192,579 - thiF BNILDI_05995 0.16 -1.6
1,192,588 + thiF BNILDI_05995 0.17 -0.9
1,192,589 - thiF BNILDI_05995 0.18 -1.1
1,192,594 - thiF BNILDI_05995 0.18 -0.0
1,192,704 - thiF BNILDI_05995 0.33 -1.9
1,192,774 - thiF BNILDI_05995 0.42 -0.1
1,192,807 - thiF BNILDI_05995 0.46 -1.0
1,192,827 + thiF BNILDI_05995 0.49 -1.6
1,192,828 - thiF BNILDI_05995 0.49 -0.5
1,192,903 + thiF BNILDI_05995 0.59 -3.2
1,192,904 - thiF BNILDI_05995 0.59 -0.0
1,192,904 - thiF BNILDI_05995 0.59 -3.1
1,192,904 - thiF BNILDI_05995 0.59 +0.4
1,192,904 - thiF BNILDI_05995 0.59 -2.8
1,193,075 + thiF BNILDI_05995 0.82 -0.3
1,193,075 + thiF BNILDI_05995 0.82 -1.5
1,193,075 + thiF BNILDI_05995 0.82 -0.8
1,193,076 - thiF BNILDI_05995 0.82 -1.9
1,193,184 + -2.0
1,193,464 + thiE BNILDI_06000 0.41 -2.8
1,193,465 - thiE BNILDI_06000 0.41 +0.5
1,193,466 + thiE BNILDI_06000 0.41 +0.2
1,193,466 + thiE BNILDI_06000 0.41 +4.5
1,193,466 + thiE BNILDI_06000 0.41 -1.8
1,193,467 - thiE BNILDI_06000 0.41 -0.2
1,193,467 - thiE BNILDI_06000 0.41 -3.2
1,193,467 - thiE BNILDI_06000 0.41 -0.9
1,193,467 - thiE BNILDI_06000 0.41 -0.7
1,193,598 + thiE BNILDI_06000 0.62 -0.3
1,193,599 - thiE BNILDI_06000 0.62 -0.3
1,193,599 - thiE BNILDI_06000 0.62 +0.2
1,193,638 + thiE BNILDI_06000 0.68 -0.6
1,193,727 - thiE BNILDI_06000 0.82 +0.3
1,193,737 + thiE BNILDI_06000 0.84 +0.1
1,193,738 - thiE BNILDI_06000 0.84 +0.3
1,193,738 - thiE BNILDI_06000 0.84 -1.8
1,193,741 + thiE BNILDI_06000 0.84 -0.6
1,193,742 - thiE BNILDI_06000 0.85 -2.4
1,193,774 + thiE BNILDI_06000 0.90 -0.5
1,193,775 - thiE BNILDI_06000 0.90 -3.6
1,193,775 - thiE BNILDI_06000 0.90 -1.5
1,193,835 + -1.1
1,193,836 - -2.2
1,193,920 - -3.2
1,193,920 - -2.6
1,193,920 - -0.2
1,194,075 + thiC BNILDI_06005 0.12 -1.0
1,194,183 + thiC BNILDI_06005 0.18 -0.7
1,194,183 + thiC BNILDI_06005 0.18 -0.3
1,194,185 + thiC BNILDI_06005 0.18 -0.1
1,194,185 + thiC BNILDI_06005 0.18 -3.4
1,194,185 + thiC BNILDI_06005 0.18 +1.9
1,194,186 - thiC BNILDI_06005 0.18 +0.1
1,194,193 - thiC BNILDI_06005 0.19 -0.1
1,194,205 - thiC BNILDI_06005 0.19 -0.2

Or see this region's nucleotide sequence