Strain Fitness in Escherichia coli ECRC62 around BNILDI_05125

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntubiB and tatA are separated by 78 nucleotidestatA and tatB are separated by 3 nucleotidestatB and tatC are separated by 2 nucleotidestatC and tatD are separated by 41 nucleotidestatD and rfaH overlap by 4 nucleotides BNILDI_05110: ubiB - ubiquinone biosynthesis regulatory protein kinase UbiB, at 1,012,238 to 1,013,878 ubiB BNILDI_05115: tatA - Sec-independent protein translocase subunit TatA, at 1,013,957 to 1,014,226 tatA BNILDI_05120: tatB - Sec-independent protein translocase protein TatB, at 1,014,230 to 1,014,745 tatB BNILDI_05125: tatC - Sec-independent protein translocase subunit TatC, at 1,014,748 to 1,015,524 tatC BNILDI_05130: tatD - 3'-5' ssDNA/RNA exonuclease TatD, at 1,015,566 to 1,016,348 tatD BNILDI_05135: rfaH - transcription/translation regulatory transformer protein RfaH, at 1,016,345 to 1,016,833 rfaH Position (kb) 1014 1015 1016Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1013.911 kb on + strandat 1013.911 kb on + strandat 1013.912 kb on - strandat 1013.912 kb on - strandat 1013.914 kb on - strandat 1013.940 kb on - strandat 1013.956 kb on + strandat 1013.956 kb on + strandat 1013.956 kb on + strandat 1013.956 kb on + strandat 1013.957 kb on - strandat 1013.957 kb on - strandat 1013.966 kb on - strandat 1013.982 kb on + strandat 1013.982 kb on + strandat 1014.006 kb on + strand, within tatAat 1014.224 kb on + strandat 1014.224 kb on + strandat 1014.224 kb on + strandat 1014.483 kb on + strand, within tatBat 1014.483 kb on + strand, within tatBat 1014.516 kb on - strand, within tatBat 1014.583 kb on + strand, within tatBat 1014.584 kb on - strand, within tatBat 1014.678 kb on + strand, within tatBat 1014.679 kb on - strand, within tatBat 1014.755 kb on + strandat 1014.756 kb on - strandat 1014.763 kb on - strandat 1014.774 kb on + strandat 1014.799 kb on - strandat 1014.854 kb on - strand, within tatCat 1014.976 kb on - strand, within tatCat 1014.976 kb on - strand, within tatCat 1014.976 kb on - strand, within tatCat 1015.012 kb on + strand, within tatCat 1015.029 kb on + strand, within tatCat 1015.046 kb on - strand, within tatCat 1015.047 kb on + strand, within tatCat 1015.047 kb on + strand, within tatCat 1015.103 kb on - strand, within tatCat 1015.103 kb on - strand, within tatCat 1015.208 kb on - strand, within tatCat 1015.211 kb on + strand, within tatCat 1015.211 kb on + strand, within tatCat 1015.297 kb on - strand, within tatCat 1015.330 kb on + strand, within tatCat 1015.330 kb on + strand, within tatCat 1015.331 kb on - strand, within tatCat 1015.331 kb on - strand, within tatCat 1015.414 kb on + strand, within tatCat 1015.414 kb on + strand, within tatCat 1015.415 kb on - strand, within tatCat 1015.415 kb on - strand, within tatCat 1015.554 kb on - strandat 1015.574 kb on + strandat 1015.574 kb on + strandat 1015.627 kb on + strandat 1015.627 kb on + strandat 1015.653 kb on - strand, within tatDat 1015.747 kb on + strand, within tatDat 1015.906 kb on + strand, within tatDat 1015.906 kb on + strand, within tatDat 1015.907 kb on - strand, within tatDat 1015.925 kb on - strand, within tatDat 1015.940 kb on + strand, within tatDat 1015.941 kb on - strand, within tatDat 1015.941 kb on - strand, within tatDat 1015.941 kb on - strand, within tatDat 1015.941 kb on - strand, within tatDat 1016.074 kb on - strand, within tatDat 1016.261 kb on + strand, within tatDat 1016.262 kb on - strand, within tatDat 1016.262 kb on - strand, within tatDat 1016.384 kb on - strandat 1016.392 kb on + strandat 1016.392 kb on + strandat 1016.395 kb on + strand, within rfaHat 1016.396 kb on - strand, within rfaHat 1016.396 kb on - strand, within rfaHat 1016.398 kb on + strand, within rfaHat 1016.399 kb on - strand, within rfaHat 1016.404 kb on + strand, within rfaHat 1016.482 kb on + strand, within rfaH

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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1,013,911 + +0.5
1,013,911 + +0.4
1,013,912 - -1.4
1,013,912 - +1.2
1,013,914 - -0.3
1,013,940 - -2.6
1,013,956 + +1.5
1,013,956 + -0.7
1,013,956 + +0.3
1,013,956 + -0.5
1,013,957 - +0.1
1,013,957 - -2.8
1,013,966 - -4.1
1,013,982 + +0.5
1,013,982 + +1.3
1,014,006 + tatA BNILDI_05115 0.18 +0.3
1,014,224 + -1.0
1,014,224 + -2.9
1,014,224 + -3.3
1,014,483 + tatB BNILDI_05120 0.49 -0.4
1,014,483 + tatB BNILDI_05120 0.49 -0.5
1,014,516 - tatB BNILDI_05120 0.55 -1.4
1,014,583 + tatB BNILDI_05120 0.68 +1.1
1,014,584 - tatB BNILDI_05120 0.69 -2.3
1,014,678 + tatB BNILDI_05120 0.87 -0.5
1,014,679 - tatB BNILDI_05120 0.87 -1.6
1,014,755 + -1.0
1,014,756 - -3.7
1,014,763 - -2.4
1,014,774 + -2.7
1,014,799 - -2.0
1,014,854 - tatC BNILDI_05125 0.14 -2.1
1,014,976 - tatC BNILDI_05125 0.29 -1.8
1,014,976 - tatC BNILDI_05125 0.29 -1.6
1,014,976 - tatC BNILDI_05125 0.29 -0.1
1,015,012 + tatC BNILDI_05125 0.34 -1.5
1,015,029 + tatC BNILDI_05125 0.36 -4.8
1,015,046 - tatC BNILDI_05125 0.38 -0.9
1,015,047 + tatC BNILDI_05125 0.38 -3.5
1,015,047 + tatC BNILDI_05125 0.38 -5.1
1,015,103 - tatC BNILDI_05125 0.46 -4.4
1,015,103 - tatC BNILDI_05125 0.46 +1.6
1,015,208 - tatC BNILDI_05125 0.59 -1.8
1,015,211 + tatC BNILDI_05125 0.60 -2.2
1,015,211 + tatC BNILDI_05125 0.60 -0.6
1,015,297 - tatC BNILDI_05125 0.71 -0.9
1,015,330 + tatC BNILDI_05125 0.75 -2.5
1,015,330 + tatC BNILDI_05125 0.75 -3.9
1,015,331 - tatC BNILDI_05125 0.75 -2.5
1,015,331 - tatC BNILDI_05125 0.75 -3.7
1,015,414 + tatC BNILDI_05125 0.86 -2.1
1,015,414 + tatC BNILDI_05125 0.86 -1.2
1,015,415 - tatC BNILDI_05125 0.86 -3.0
1,015,415 - tatC BNILDI_05125 0.86 -0.9
1,015,554 - -1.0
1,015,574 + -1.0
1,015,574 + -2.2
1,015,627 + -0.3
1,015,627 + -0.7
1,015,653 - tatD BNILDI_05130 0.11 +0.3
1,015,747 + tatD BNILDI_05130 0.23 +1.5
1,015,906 + tatD BNILDI_05130 0.43 +0.1
1,015,906 + tatD BNILDI_05130 0.43 +0.7
1,015,907 - tatD BNILDI_05130 0.44 -1.5
1,015,925 - tatD BNILDI_05130 0.46 +1.3
1,015,940 + tatD BNILDI_05130 0.48 +0.4
1,015,941 - tatD BNILDI_05130 0.48 -0.4
1,015,941 - tatD BNILDI_05130 0.48 +0.1
1,015,941 - tatD BNILDI_05130 0.48 -0.0
1,015,941 - tatD BNILDI_05130 0.48 +0.5
1,016,074 - tatD BNILDI_05130 0.65 -1.6
1,016,261 + tatD BNILDI_05130 0.89 +0.6
1,016,262 - tatD BNILDI_05130 0.89 +0.3
1,016,262 - tatD BNILDI_05130 0.89 -0.3
1,016,384 - -2.0
1,016,392 + -0.4
1,016,392 + +0.3
1,016,395 + rfaH BNILDI_05135 0.10 +0.3
1,016,396 - rfaH BNILDI_05135 0.10 -1.5
1,016,396 - rfaH BNILDI_05135 0.10 -0.1
1,016,398 + rfaH BNILDI_05135 0.11 +0.5
1,016,399 - rfaH BNILDI_05135 0.11 +0.2
1,016,404 + rfaH BNILDI_05135 0.12 +0.2
1,016,482 + rfaH BNILDI_05135 0.28 +0.2

Or see this region's nucleotide sequence