Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ubiB and tatA are separated by 78 nucleotides tatA and tatB are separated by 3 nucleotides tatB and tatC are separated by 2 nucleotides tatC and tatD are separated by 41 nucleotides tatD and rfaH overlap by 4 nucleotides
BNILDI_05110: ubiB - ubiquinone biosynthesis regulatory protein kinase UbiB, at 1,012,238 to 1,013,878
ubiB
BNILDI_05115: tatA - Sec-independent protein translocase subunit TatA, at 1,013,957 to 1,014,226
tatA
BNILDI_05120: tatB - Sec-independent protein translocase protein TatB, at 1,014,230 to 1,014,745
tatB
BNILDI_05125: tatC - Sec-independent protein translocase subunit TatC, at 1,014,748 to 1,015,524
tatC
BNILDI_05130: tatD - 3'-5' ssDNA/RNA exonuclease TatD, at 1,015,566 to 1,016,348
tatD
BNILDI_05135: rfaH - transcription/translation regulatory transformer protein RfaH, at 1,016,345 to 1,016,833
rfaH
Position (kb)
1014
1015
1016 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1013.911 kb on + strand at 1013.911 kb on + strand at 1013.912 kb on - strand at 1013.912 kb on - strand at 1013.914 kb on - strand at 1013.940 kb on - strand at 1013.956 kb on + strand at 1013.956 kb on + strand at 1013.956 kb on + strand at 1013.956 kb on + strand at 1013.957 kb on - strand at 1013.957 kb on - strand at 1013.966 kb on - strand at 1013.982 kb on + strand at 1013.982 kb on + strand at 1014.006 kb on + strand, within tatA at 1014.224 kb on + strand at 1014.224 kb on + strand at 1014.224 kb on + strand at 1014.483 kb on + strand, within tatB at 1014.483 kb on + strand, within tatB at 1014.516 kb on - strand, within tatB at 1014.583 kb on + strand, within tatB at 1014.584 kb on - strand, within tatB at 1014.678 kb on + strand, within tatB at 1014.679 kb on - strand, within tatB at 1014.755 kb on + strand at 1014.756 kb on - strand at 1014.763 kb on - strand at 1014.774 kb on + strand at 1014.799 kb on - strand at 1014.854 kb on - strand, within tatC at 1014.976 kb on - strand, within tatC at 1014.976 kb on - strand, within tatC at 1014.976 kb on - strand, within tatC at 1015.012 kb on + strand, within tatC at 1015.029 kb on + strand, within tatC at 1015.046 kb on - strand, within tatC at 1015.047 kb on + strand, within tatC at 1015.047 kb on + strand, within tatC at 1015.103 kb on - strand, within tatC at 1015.103 kb on - strand, within tatC at 1015.208 kb on - strand, within tatC at 1015.211 kb on + strand, within tatC at 1015.211 kb on + strand, within tatC at 1015.297 kb on - strand, within tatC at 1015.330 kb on + strand, within tatC at 1015.330 kb on + strand, within tatC at 1015.331 kb on - strand, within tatC at 1015.331 kb on - strand, within tatC at 1015.414 kb on + strand, within tatC at 1015.414 kb on + strand, within tatC at 1015.415 kb on - strand, within tatC at 1015.415 kb on - strand, within tatC at 1015.554 kb on - strand at 1015.574 kb on + strand at 1015.574 kb on + strand at 1015.627 kb on + strand at 1015.627 kb on + strand at 1015.653 kb on - strand, within tatD at 1015.747 kb on + strand, within tatD at 1015.906 kb on + strand, within tatD at 1015.906 kb on + strand, within tatD at 1015.907 kb on - strand, within tatD at 1015.925 kb on - strand, within tatD at 1015.940 kb on + strand, within tatD at 1015.941 kb on - strand, within tatD at 1015.941 kb on - strand, within tatD at 1015.941 kb on - strand, within tatD at 1015.941 kb on - strand, within tatD at 1016.074 kb on - strand, within tatD at 1016.261 kb on + strand, within tatD at 1016.262 kb on - strand, within tatD at 1016.262 kb on - strand, within tatD at 1016.384 kb on - strand at 1016.392 kb on + strand at 1016.392 kb on + strand at 1016.395 kb on + strand, within rfaH at 1016.396 kb on - strand, within rfaH at 1016.396 kb on - strand, within rfaH at 1016.398 kb on + strand, within rfaH at 1016.399 kb on - strand, within rfaH at 1016.404 kb on + strand, within rfaH at 1016.482 kb on + strand, within rfaH
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,013,911 + +0.5 1,013,911 + +0.4 1,013,912 - -1.4 1,013,912 - +1.2 1,013,914 - -0.3 1,013,940 - -2.6 1,013,956 + +1.5 1,013,956 + -0.7 1,013,956 + +0.3 1,013,956 + -0.5 1,013,957 - +0.1 1,013,957 - -2.8 1,013,966 - -4.1 1,013,982 + +0.5 1,013,982 + +1.3 1,014,006 + tatA BNILDI_05115 0.18 +0.3 1,014,224 + -1.0 1,014,224 + -2.9 1,014,224 + -3.3 1,014,483 + tatB BNILDI_05120 0.49 -0.4 1,014,483 + tatB BNILDI_05120 0.49 -0.5 1,014,516 - tatB BNILDI_05120 0.55 -1.4 1,014,583 + tatB BNILDI_05120 0.68 +1.1 1,014,584 - tatB BNILDI_05120 0.69 -2.3 1,014,678 + tatB BNILDI_05120 0.87 -0.5 1,014,679 - tatB BNILDI_05120 0.87 -1.6 1,014,755 + -1.0 1,014,756 - -3.7 1,014,763 - -2.4 1,014,774 + -2.7 1,014,799 - -2.0 1,014,854 - tatC BNILDI_05125 0.14 -2.1 1,014,976 - tatC BNILDI_05125 0.29 -1.8 1,014,976 - tatC BNILDI_05125 0.29 -1.6 1,014,976 - tatC BNILDI_05125 0.29 -0.1 1,015,012 + tatC BNILDI_05125 0.34 -1.5 1,015,029 + tatC BNILDI_05125 0.36 -4.8 1,015,046 - tatC BNILDI_05125 0.38 -0.9 1,015,047 + tatC BNILDI_05125 0.38 -3.5 1,015,047 + tatC BNILDI_05125 0.38 -5.1 1,015,103 - tatC BNILDI_05125 0.46 -4.4 1,015,103 - tatC BNILDI_05125 0.46 +1.6 1,015,208 - tatC BNILDI_05125 0.59 -1.8 1,015,211 + tatC BNILDI_05125 0.60 -2.2 1,015,211 + tatC BNILDI_05125 0.60 -0.6 1,015,297 - tatC BNILDI_05125 0.71 -0.9 1,015,330 + tatC BNILDI_05125 0.75 -2.5 1,015,330 + tatC BNILDI_05125 0.75 -3.9 1,015,331 - tatC BNILDI_05125 0.75 -2.5 1,015,331 - tatC BNILDI_05125 0.75 -3.7 1,015,414 + tatC BNILDI_05125 0.86 -2.1 1,015,414 + tatC BNILDI_05125 0.86 -1.2 1,015,415 - tatC BNILDI_05125 0.86 -3.0 1,015,415 - tatC BNILDI_05125 0.86 -0.9 1,015,554 - -1.0 1,015,574 + -1.0 1,015,574 + -2.2 1,015,627 + -0.3 1,015,627 + -0.7 1,015,653 - tatD BNILDI_05130 0.11 +0.3 1,015,747 + tatD BNILDI_05130 0.23 +1.5 1,015,906 + tatD BNILDI_05130 0.43 +0.1 1,015,906 + tatD BNILDI_05130 0.43 +0.7 1,015,907 - tatD BNILDI_05130 0.44 -1.5 1,015,925 - tatD BNILDI_05130 0.46 +1.3 1,015,940 + tatD BNILDI_05130 0.48 +0.4 1,015,941 - tatD BNILDI_05130 0.48 -0.4 1,015,941 - tatD BNILDI_05130 0.48 +0.1 1,015,941 - tatD BNILDI_05130 0.48 -0.0 1,015,941 - tatD BNILDI_05130 0.48 +0.5 1,016,074 - tatD BNILDI_05130 0.65 -1.6 1,016,261 + tatD BNILDI_05130 0.89 +0.6 1,016,262 - tatD BNILDI_05130 0.89 +0.3 1,016,262 - tatD BNILDI_05130 0.89 -0.3 1,016,384 - -2.0 1,016,392 + -0.4 1,016,392 + +0.3 1,016,395 + rfaH BNILDI_05135 0.10 +0.3 1,016,396 - rfaH BNILDI_05135 0.10 -1.5 1,016,396 - rfaH BNILDI_05135 0.10 -0.1 1,016,398 + rfaH BNILDI_05135 0.11 +0.5 1,016,399 - rfaH BNILDI_05135 0.11 +0.2 1,016,404 + rfaH BNILDI_05135 0.12 +0.2 1,016,482 + rfaH BNILDI_05135 0.28 +0.2
Or see this region's nucleotide sequence