Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yidE and ibpB are separated by 196 nucleotides ibpB and ibpA are separated by 111 nucleotides ibpA and yidQ are separated by 377 nucleotides yidQ and yidR are separated by 1 nucleotides
BNILDI_04285: yidE - putative transporter, at 847,089 to 848,750
yidE
BNILDI_04290: ibpB - heat shock chaperone IbpB, at 848,947 to 849,375
ibpB
BNILDI_04295: ibpA - heat shock chaperone IbpA, at 849,487 to 849,900
ibpA
BNILDI_04300: yidQ - Uncharacterized protein YidQ, at 850,278 to 850,538
yidQ
BNILDI_04305: yidR - Uncharacterized protein YidR, at 850,540 to 851,754
yidR
Position (kb) 849
850 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 848.534 kb on + strand, within yidE at 848.534 kb on - strand, within yidE at 848.536 kb on + strand, within yidE at 848.589 kb on + strand at 848.589 kb on + strand at 848.590 kb on - strand at 848.590 kb on - strand at 848.590 kb on - strand at 848.655 kb on - strand at 848.658 kb on + strand at 848.658 kb on + strand at 848.659 kb on - strand at 848.664 kb on + strand at 848.739 kb on + strand at 848.739 kb on + strand at 848.740 kb on - strand at 848.740 kb on - strand at 848.825 kb on - strand at 848.860 kb on - strand at 848.899 kb on - strand at 848.940 kb on + strand at 848.940 kb on + strand at 848.940 kb on + strand at 848.945 kb on - strand at 848.945 kb on - strand at 848.945 kb on - strand at 848.948 kb on + strand at 848.948 kb on + strand at 848.952 kb on + strand at 849.166 kb on + strand, within ibpB at 849.272 kb on + strand, within ibpB at 849.391 kb on + strand at 849.391 kb on + strand at 849.403 kb on - strand at 849.407 kb on - strand at 849.407 kb on - strand at 849.430 kb on + strand at 849.489 kb on - strand at 849.548 kb on + strand, within ibpA at 849.549 kb on - strand, within ibpA at 849.549 kb on - strand, within ibpA at 849.549 kb on - strand, within ibpA at 849.563 kb on - strand, within ibpA at 849.641 kb on + strand, within ibpA at 849.647 kb on + strand, within ibpA at 849.647 kb on + strand, within ibpA at 849.647 kb on + strand, within ibpA at 849.648 kb on - strand, within ibpA at 849.792 kb on - strand, within ibpA at 849.810 kb on + strand, within ibpA at 849.895 kb on - strand at 849.908 kb on - strand at 849.908 kb on - strand at 849.932 kb on - strand at 849.959 kb on + strand at 850.061 kb on - strand at 850.067 kb on + strand at 850.164 kb on - strand at 850.164 kb on - strand at 850.164 kb on - strand at 850.202 kb on + strand at 850.205 kb on + strand at 850.206 kb on - strand at 850.206 kb on - strand at 850.237 kb on + strand at 850.367 kb on + strand, within yidQ at 850.367 kb on + strand, within yidQ at 850.532 kb on + strand at 850.533 kb on - strand at 850.658 kb on + strand at 850.658 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 848,534 + yidE BNILDI_04285 0.87 +0.6 848,534 - yidE BNILDI_04285 0.87 -0.6 848,536 + yidE BNILDI_04285 0.87 -1.6 848,589 + +1.4 848,589 + -2.2 848,590 - +0.2 848,590 - +0.8 848,590 - -2.7 848,655 - +1.3 848,658 + -0.3 848,658 + +0.3 848,659 - -1.1 848,664 + +0.0 848,739 + -0.8 848,739 + +0.3 848,740 - +0.2 848,740 - -0.1 848,825 - -0.9 848,860 - +0.5 848,899 - -2.9 848,940 + +0.4 848,940 + +0.2 848,940 + -2.1 848,945 - +1.5 848,945 - -0.2 848,945 - +0.4 848,948 + +0.1 848,948 + -2.3 848,952 + -0.7 849,166 + ibpB BNILDI_04290 0.51 +1.1 849,272 + ibpB BNILDI_04290 0.76 -1.0 849,391 + +1.6 849,391 + -1.1 849,403 - -0.0 849,407 - +0.6 849,407 - -2.4 849,430 + +0.2 849,489 - +0.5 849,548 + ibpA BNILDI_04295 0.15 -0.7 849,549 - ibpA BNILDI_04295 0.15 +0.5 849,549 - ibpA BNILDI_04295 0.15 +0.4 849,549 - ibpA BNILDI_04295 0.15 -0.7 849,563 - ibpA BNILDI_04295 0.18 -0.0 849,641 + ibpA BNILDI_04295 0.37 +0.1 849,647 + ibpA BNILDI_04295 0.39 -0.2 849,647 + ibpA BNILDI_04295 0.39 -2.5 849,647 + ibpA BNILDI_04295 0.39 -0.0 849,648 - ibpA BNILDI_04295 0.39 -0.2 849,792 - ibpA BNILDI_04295 0.74 -0.3 849,810 + ibpA BNILDI_04295 0.78 -1.8 849,895 - +0.5 849,908 - -0.3 849,908 - +0.9 849,932 - -0.2 849,959 + +0.4 850,061 - -3.6 850,067 + -0.5 850,164 - -2.8 850,164 - -0.9 850,164 - +0.3 850,202 + +1.1 850,205 + -1.5 850,206 - -1.8 850,206 - +0.4 850,237 + -0.5 850,367 + yidQ BNILDI_04300 0.34 +0.1 850,367 + yidQ BNILDI_04300 0.34 +0.4 850,532 + -0.1 850,533 - +0.2 850,658 + -0.0 850,658 + -1.1
Or see this region's nucleotide sequence