Strain Fitness in Escherichia coli ECRC62 around BNILDI_04085

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntspoT and trmH are separated by 6 nucleotidestrmH and recG are separated by 5 nucleotides BNILDI_04080: spoT - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 804,055 to 806,163 spoT BNILDI_04085: trmH - tRNA (guanosine(18)-2'-O)-methyltransferase TrmH, at 806,170 to 806,859 trmH BNILDI_04090: recG - ATP-dependent DNA helicase RecG, at 806,865 to 808,946 recG Position (kb) 806 807Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 805.676 kb on + strand, within spoTat 805.995 kb on + strandat 806.103 kb on + strandat 806.169 kb on + strandat 806.170 kb on - strandat 806.185 kb on + strandat 806.370 kb on + strand, within trmHat 806.370 kb on + strand, within trmHat 806.371 kb on - strand, within trmHat 806.680 kb on + strand, within trmHat 806.839 kb on - strandat 806.841 kb on + strandat 806.841 kb on + strandat 806.858 kb on - strandat 806.858 kb on - strandat 806.882 kb on - strandat 806.882 kb on - strandat 806.927 kb on + strandat 806.958 kb on - strandat 806.972 kb on - strandat 806.977 kb on + strandat 806.977 kb on + strandat 806.977 kb on + strandat 807.031 kb on + strandat 807.031 kb on + strandat 807.080 kb on + strand, within recGat 807.081 kb on - strand, within recGat 807.081 kb on - strand, within recGat 807.356 kb on + strand, within recGat 807.362 kb on - strand, within recGat 807.580 kb on + strand, within recGat 807.581 kb on - strand, within recGat 807.642 kb on - strand, within recGat 807.757 kb on + strand, within recGat 807.757 kb on + strand, within recGat 807.757 kb on + strand, within recGat 807.757 kb on + strand, within recGat 807.767 kb on + strand, within recGat 807.823 kb on + strand, within recGat 807.824 kb on - strand, within recGat 807.824 kb on - strand, within recG

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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805,676 + spoT BNILDI_04080 0.77 -0.0
805,995 + -3.0
806,103 + +1.5
806,169 + -0.4
806,170 - +0.6
806,185 + -0.3
806,370 + trmH BNILDI_04085 0.29 +0.3
806,370 + trmH BNILDI_04085 0.29 -1.1
806,371 - trmH BNILDI_04085 0.29 -0.8
806,680 + trmH BNILDI_04085 0.74 -2.4
806,839 - -2.6
806,841 + +0.5
806,841 + +0.3
806,858 - +0.4
806,858 - -1.2
806,882 - -0.4
806,882 - -0.3
806,927 + +0.9
806,958 - -4.2
806,972 - -0.6
806,977 + -2.5
806,977 + -1.8
806,977 + +0.8
807,031 + -0.3
807,031 + +0.1
807,080 + recG BNILDI_04090 0.10 -0.7
807,081 - recG BNILDI_04090 0.10 -3.3
807,081 - recG BNILDI_04090 0.10 +0.7
807,356 + recG BNILDI_04090 0.24 -0.6
807,362 - recG BNILDI_04090 0.24 -0.5
807,580 + recG BNILDI_04090 0.34 +0.4
807,581 - recG BNILDI_04090 0.34 -0.1
807,642 - recG BNILDI_04090 0.37 -2.2
807,757 + recG BNILDI_04090 0.43 +1.3
807,757 + recG BNILDI_04090 0.43 -2.5
807,757 + recG BNILDI_04090 0.43 -1.3
807,757 + recG BNILDI_04090 0.43 -3.2
807,767 + recG BNILDI_04090 0.43 +0.3
807,823 + recG BNILDI_04090 0.46 -3.2
807,824 - recG BNILDI_04090 0.46 -0.6
807,824 - recG BNILDI_04090 0.46 -1.5

Or see this region's nucleotide sequence