Strain Fitness in Escherichia coli ECRC62 around BNILDI_03535

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBNILDI_03530 and BNILDI_03535 overlap by 125 nucleotidesBNILDI_03535 and Pro_trna are separated by 48 nucleotidesPro_trna and eptB are separated by 91 nucleotides BNILDI_03530: BNILDI_03530 - IS110-like element IS621 family transposase, at 689,221 to 690,201 _03530 BNILDI_03535: BNILDI_03535 - hypothetical protein, at 690,077 to 690,688 _03535 BNILDI_03540: Pro_trna - tRNA-Pro(cgg), at 690,737 to 690,813 _trna BNILDI_03545: eptB - kdo(2)-lipid A phosphoethanolamine 7''-transferase, at 690,905 to 692,596 eptB Position (kb) 690 691Strain fitness (log2 ratio) -3 -2 -1 0 1at 690.308 kb on + strand, within BNILDI_03535at 690.907 kb on - strandat 690.983 kb on - strandat 690.983 kb on - strandat 690.985 kb on - strandat 690.985 kb on - strandat 691.019 kb on + strandat 691.020 kb on - strandat 691.098 kb on + strand, within eptBat 691.103 kb on - strand, within eptBat 691.189 kb on + strand, within eptBat 691.215 kb on + strand, within eptBat 691.264 kb on + strand, within eptBat 691.265 kb on - strand, within eptBat 691.278 kb on + strand, within eptBat 691.278 kb on + strand, within eptBat 691.279 kb on - strand, within eptBat 691.279 kb on - strand, within eptBat 691.279 kb on - strand, within eptBat 691.342 kb on + strand, within eptBat 691.343 kb on - strand, within eptBat 691.369 kb on + strand, within eptBat 691.390 kb on - strand, within eptBat 691.390 kb on - strand, within eptBat 691.390 kb on - strand, within eptBat 691.483 kb on + strand, within eptBat 691.483 kb on + strand, within eptBat 691.484 kb on - strand, within eptBat 691.593 kb on + strand, within eptBat 691.613 kb on + strand, within eptBat 691.614 kb on - strand, within eptBat 691.650 kb on - strand, within eptBat 691.650 kb on - strand, within eptBat 691.666 kb on + strand, within eptB

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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690,308 + BNILDI_03535 0.38 -3.3
690,907 - -0.2
690,983 - -0.0
690,983 - +0.2
690,985 - -1.2
690,985 - -0.4
691,019 + -1.6
691,020 - -1.1
691,098 + eptB BNILDI_03545 0.11 +1.2
691,103 - eptB BNILDI_03545 0.12 -0.2
691,189 + eptB BNILDI_03545 0.17 -3.6
691,215 + eptB BNILDI_03545 0.18 +0.5
691,264 + eptB BNILDI_03545 0.21 -1.4
691,265 - eptB BNILDI_03545 0.21 +0.9
691,278 + eptB BNILDI_03545 0.22 +0.0
691,278 + eptB BNILDI_03545 0.22 -1.3
691,279 - eptB BNILDI_03545 0.22 +0.0
691,279 - eptB BNILDI_03545 0.22 +0.4
691,279 - eptB BNILDI_03545 0.22 +0.5
691,342 + eptB BNILDI_03545 0.26 -0.8
691,343 - eptB BNILDI_03545 0.26 +1.5
691,369 + eptB BNILDI_03545 0.27 -1.2
691,390 - eptB BNILDI_03545 0.29 -1.3
691,390 - eptB BNILDI_03545 0.29 +1.0
691,390 - eptB BNILDI_03545 0.29 -1.4
691,483 + eptB BNILDI_03545 0.34 +0.3
691,483 + eptB BNILDI_03545 0.34 -2.7
691,484 - eptB BNILDI_03545 0.34 -2.6
691,593 + eptB BNILDI_03545 0.41 +0.4
691,613 + eptB BNILDI_03545 0.42 -0.3
691,614 - eptB BNILDI_03545 0.42 -2.6
691,650 - eptB BNILDI_03545 0.44 +0.2
691,650 - eptB BNILDI_03545 0.44 +0.7
691,666 + eptB BNILDI_03545 0.45 +0.9

Or see this region's nucleotide sequence