Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dtpB and rsmJ are separated by 48 nucleotides rsmJ and prlC are separated by 7 nucleotides prlC and rlmJ are separated by 202 nucleotides
BNILDI_03200: dtpB - dipeptide/tripeptide permease DtpB, at 620,457 to 621,926
dtpB
BNILDI_03205: rsmJ - 16S rRNA (guanine(1516)-N(2))-methyltransferase RsmJ, at 621,975 to 622,727
rsmJ
BNILDI_03210: prlC - oligopeptidase A, at 622,735 to 624,777
prlC
BNILDI_03215: rlmJ - 23S rRNA (adenine(2030)-N(6))-methyltransferase, at 624,980 to 625,822
rlmJ
Position (kb) 622
623
624
625 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 621.754 kb on - strand, within dtpB at 621.816 kb on + strand at 621.816 kb on + strand at 621.817 kb on - strand at 621.834 kb on - strand at 621.866 kb on - strand at 621.924 kb on + strand at 621.928 kb on + strand at 621.977 kb on - strand at 622.007 kb on + strand at 622.007 kb on + strand at 622.008 kb on - strand at 622.077 kb on + strand, within rsmJ at 622.078 kb on - strand, within rsmJ at 622.119 kb on + strand, within rsmJ at 622.120 kb on - strand, within rsmJ at 622.281 kb on + strand, within rsmJ at 622.281 kb on + strand, within rsmJ at 622.282 kb on - strand, within rsmJ at 622.681 kb on - strand at 622.921 kb on + strand at 622.937 kb on + strand at 622.937 kb on + strand at 622.937 kb on + strand at 622.943 kb on + strand, within prlC at 622.943 kb on + strand, within prlC at 622.946 kb on + strand, within prlC at 622.959 kb on - strand, within prlC at 623.018 kb on - strand, within prlC at 623.137 kb on - strand, within prlC at 623.154 kb on - strand, within prlC at 623.226 kb on + strand, within prlC at 623.346 kb on + strand, within prlC at 623.346 kb on + strand, within prlC at 623.416 kb on - strand, within prlC at 623.416 kb on - strand, within prlC at 623.485 kb on - strand, within prlC at 623.544 kb on - strand, within prlC at 623.544 kb on - strand, within prlC at 623.544 kb on - strand, within prlC at 623.588 kb on + strand, within prlC at 623.589 kb on - strand, within prlC at 623.606 kb on - strand, within prlC at 623.635 kb on - strand, within prlC at 623.658 kb on - strand, within prlC at 623.658 kb on - strand, within prlC at 623.741 kb on + strand, within prlC at 623.741 kb on + strand, within prlC at 623.741 kb on + strand, within prlC at 623.742 kb on - strand, within prlC at 623.768 kb on + strand, within prlC at 623.769 kb on - strand, within prlC at 623.938 kb on - strand, within prlC at 623.945 kb on + strand, within prlC at 623.946 kb on - strand, within prlC at 623.946 kb on - strand, within prlC at 624.025 kb on - strand, within prlC at 624.071 kb on + strand, within prlC at 624.072 kb on - strand, within prlC at 624.104 kb on + strand, within prlC at 624.105 kb on - strand, within prlC at 624.457 kb on - strand, within prlC at 624.492 kb on - strand, within prlC at 624.492 kb on - strand, within prlC at 624.521 kb on + strand, within prlC at 624.582 kb on + strand at 624.582 kb on + strand at 624.582 kb on + strand at 624.583 kb on - strand at 624.583 kb on - strand at 624.583 kb on - strand at 624.638 kb on + strand at 624.638 kb on + strand at 624.639 kb on - strand at 624.827 kb on - strand at 624.830 kb on + strand at 624.830 kb on + strand at 624.831 kb on - strand at 624.851 kb on - strand at 624.851 kb on - strand at 624.879 kb on + strand at 624.911 kb on + strand at 624.911 kb on + strand at 624.912 kb on - strand at 624.944 kb on - strand at 625.036 kb on + strand at 625.037 kb on - strand at 625.078 kb on + strand, within rlmJ at 625.161 kb on - strand, within rlmJ at 625.299 kb on + strand, within rlmJ at 625.553 kb on + strand, within rlmJ
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 621,754 - dtpB BNILDI_03200 0.88 +1.1 621,816 + +0.6 621,816 + +1.2 621,817 - +1.5 621,834 - -3.4 621,866 - -2.4 621,924 + -0.7 621,928 + -0.4 621,977 - +0.2 622,007 + +0.1 622,007 + -0.5 622,008 - -0.9 622,077 + rsmJ BNILDI_03205 0.14 -0.2 622,078 - rsmJ BNILDI_03205 0.14 +2.3 622,119 + rsmJ BNILDI_03205 0.19 -0.2 622,120 - rsmJ BNILDI_03205 0.19 -0.2 622,281 + rsmJ BNILDI_03205 0.41 +0.9 622,281 + rsmJ BNILDI_03205 0.41 -1.3 622,282 - rsmJ BNILDI_03205 0.41 +0.0 622,681 - -3.3 622,921 + -1.4 622,937 + -0.4 622,937 + -0.3 622,937 + -0.1 622,943 + prlC BNILDI_03210 0.10 +0.6 622,943 + prlC BNILDI_03210 0.10 +0.1 622,946 + prlC BNILDI_03210 0.10 +0.5 622,959 - prlC BNILDI_03210 0.11 +0.1 623,018 - prlC BNILDI_03210 0.14 +0.0 623,137 - prlC BNILDI_03210 0.20 +0.3 623,154 - prlC BNILDI_03210 0.21 -0.3 623,226 + prlC BNILDI_03210 0.24 +1.0 623,346 + prlC BNILDI_03210 0.30 +0.6 623,346 + prlC BNILDI_03210 0.30 -1.1 623,416 - prlC BNILDI_03210 0.33 +0.2 623,416 - prlC BNILDI_03210 0.33 -0.4 623,485 - prlC BNILDI_03210 0.37 -3.9 623,544 - prlC BNILDI_03210 0.40 -0.7 623,544 - prlC BNILDI_03210 0.40 -0.5 623,544 - prlC BNILDI_03210 0.40 +0.3 623,588 + prlC BNILDI_03210 0.42 -0.2 623,589 - prlC BNILDI_03210 0.42 -0.6 623,606 - prlC BNILDI_03210 0.43 +0.3 623,635 - prlC BNILDI_03210 0.44 -3.8 623,658 - prlC BNILDI_03210 0.45 -0.9 623,658 - prlC BNILDI_03210 0.45 -0.1 623,741 + prlC BNILDI_03210 0.49 -0.5 623,741 + prlC BNILDI_03210 0.49 +0.2 623,741 + prlC BNILDI_03210 0.49 +1.2 623,742 - prlC BNILDI_03210 0.49 -0.0 623,768 + prlC BNILDI_03210 0.51 -1.3 623,769 - prlC BNILDI_03210 0.51 +0.2 623,938 - prlC BNILDI_03210 0.59 -0.7 623,945 + prlC BNILDI_03210 0.59 -3.1 623,946 - prlC BNILDI_03210 0.59 -0.3 623,946 - prlC BNILDI_03210 0.59 -0.5 624,025 - prlC BNILDI_03210 0.63 +0.0 624,071 + prlC BNILDI_03210 0.65 -1.2 624,072 - prlC BNILDI_03210 0.65 -2.4 624,104 + prlC BNILDI_03210 0.67 +1.2 624,105 - prlC BNILDI_03210 0.67 -0.5 624,457 - prlC BNILDI_03210 0.84 -0.9 624,492 - prlC BNILDI_03210 0.86 -1.2 624,492 - prlC BNILDI_03210 0.86 -0.4 624,521 + prlC BNILDI_03210 0.87 -0.9 624,582 + +0.4 624,582 + -2.3 624,582 + +0.5 624,583 - -4.1 624,583 - -0.6 624,583 - -2.5 624,638 + -2.0 624,638 + -3.8 624,639 - -0.2 624,827 - -0.3 624,830 + -0.6 624,830 + -1.3 624,831 - -0.3 624,851 - -0.3 624,851 - -0.2 624,879 + -2.6 624,911 + +0.4 624,911 + +0.6 624,912 - -2.5 624,944 - -0.3 625,036 + +0.2 625,037 - -0.6 625,078 + rlmJ BNILDI_03215 0.12 -0.3 625,161 - rlmJ BNILDI_03215 0.21 -0.5 625,299 + rlmJ BNILDI_03215 0.38 +0.2 625,553 + rlmJ BNILDI_03215 0.68 +0.1
Or see this region's nucleotide sequence