Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt livK and panM are separated by 423 nucleotides panM and rimI overlap by 4 nucleotides rimI and BNILDI_02985 are separated by 6 nucleotides BNILDI_02985 and livJ are separated by 149 nucleotides
BNILDI_02970: livK - high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK, at 575,568 to 576,677
livK
BNILDI_02975: panM - aspartate 1-decarboxylase autocleavage activator PanM, at 577,101 to 577,484
panM
BNILDI_02980: rimI - N-acetyltransferase, at 577,481 to 578,008
rimI
BNILDI_02985: BNILDI_02985 - DUF1778 domain-containing protein, at 578,015 to 578,281
_02985
BNILDI_02990: livJ - branched chain amino acid ABC transporter substrate-binding protein LivJ, at 578,431 to 579,528
livJ
Position (kb) 577
578 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 576.161 kb on - strand, within livK at 576.260 kb on + strand, within livK at 576.267 kb on - strand, within livK at 576.330 kb on - strand, within livK at 576.343 kb on + strand, within livK at 576.348 kb on - strand, within livK at 576.348 kb on - strand, within livK at 576.394 kb on + strand, within livK at 576.394 kb on + strand, within livK at 576.399 kb on - strand, within livK at 576.399 kb on - strand, within livK at 576.492 kb on - strand, within livK at 576.509 kb on + strand, within livK at 576.987 kb on + strand at 577.215 kb on - strand, within panM at 577.215 kb on - strand, within panM at 577.218 kb on + strand, within panM at 577.268 kb on - strand, within panM at 577.433 kb on + strand, within panM at 577.562 kb on - strand, within rimI at 577.578 kb on + strand, within rimI at 577.579 kb on - strand, within rimI at 577.628 kb on - strand, within rimI at 577.628 kb on - strand, within rimI at 577.628 kb on - strand, within rimI at 577.628 kb on - strand, within rimI at 577.736 kb on - strand, within rimI at 577.818 kb on - strand, within rimI at 577.824 kb on + strand, within rimI at 577.825 kb on - strand, within rimI at 577.869 kb on + strand, within rimI at 577.869 kb on + strand, within rimI at 577.869 kb on + strand, within rimI at 577.869 kb on + strand, within rimI at 577.870 kb on - strand, within rimI at 577.929 kb on - strand, within rimI at 577.966 kb on - strand at 577.966 kb on - strand at 577.992 kb on - strand at 578.016 kb on + strand at 578.176 kb on + strand, within BNILDI_02985 at 578.176 kb on + strand, within BNILDI_02985 at 578.201 kb on + strand, within BNILDI_02985 at 578.309 kb on + strand at 578.350 kb on - strand at 578.421 kb on + strand at 578.422 kb on - strand at 578.426 kb on - strand at 578.474 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 576,161 - livK BNILDI_02970 0.53 -3.6 576,260 + livK BNILDI_02970 0.62 +0.6 576,267 - livK BNILDI_02970 0.63 -0.2 576,330 - livK BNILDI_02970 0.69 +1.4 576,343 + livK BNILDI_02970 0.70 -4.2 576,348 - livK BNILDI_02970 0.70 -0.0 576,348 - livK BNILDI_02970 0.70 -0.3 576,394 + livK BNILDI_02970 0.74 +1.1 576,394 + livK BNILDI_02970 0.74 +0.1 576,399 - livK BNILDI_02970 0.75 -2.3 576,399 - livK BNILDI_02970 0.75 +2.0 576,492 - livK BNILDI_02970 0.83 -0.1 576,509 + livK BNILDI_02970 0.85 +0.1 576,987 + -0.6 577,215 - panM BNILDI_02975 0.30 -4.2 577,215 - panM BNILDI_02975 0.30 -2.1 577,218 + panM BNILDI_02975 0.30 -1.9 577,268 - panM BNILDI_02975 0.43 +0.1 577,433 + panM BNILDI_02975 0.86 -2.6 577,562 - rimI BNILDI_02980 0.15 +0.4 577,578 + rimI BNILDI_02980 0.18 -0.3 577,579 - rimI BNILDI_02980 0.19 +0.0 577,628 - rimI BNILDI_02980 0.28 +0.1 577,628 - rimI BNILDI_02980 0.28 +0.4 577,628 - rimI BNILDI_02980 0.28 +0.3 577,628 - rimI BNILDI_02980 0.28 -0.7 577,736 - rimI BNILDI_02980 0.48 +0.5 577,818 - rimI BNILDI_02980 0.64 -3.7 577,824 + rimI BNILDI_02980 0.65 +0.1 577,825 - rimI BNILDI_02980 0.65 -3.3 577,869 + rimI BNILDI_02980 0.73 -0.3 577,869 + rimI BNILDI_02980 0.73 +1.0 577,869 + rimI BNILDI_02980 0.73 -3.0 577,869 + rimI BNILDI_02980 0.73 -1.6 577,870 - rimI BNILDI_02980 0.74 -1.1 577,929 - rimI BNILDI_02980 0.85 -0.0 577,966 - +0.4 577,966 - +0.1 577,992 - +1.3 578,016 + +1.0 578,176 + BNILDI_02985 0.60 -0.0 578,176 + BNILDI_02985 0.60 -0.1 578,201 + BNILDI_02985 0.70 -1.0 578,309 + -2.6 578,350 - -0.1 578,421 + -2.1 578,422 - -0.4 578,426 - +0.7 578,474 - -0.2
Or see this region's nucleotide sequence