Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rtcB and rtcR are separated by 188 nucleotides rtcR and glpR overlap by 19 nucleotides glpR and glpG are separated by 16 nucleotides
BNILDI_02770: rtcB - RNA-splicing ligase RtcB, at 533,578 to 534,804
rtcB
BNILDI_02775: rtcR - DNA-binding transcriptional regulator RtcR, at 534,993 to 536,591
rtcR
BNILDI_02780: glpR - DeoR/GlpR family transcriptional regulator, at 536,573 to 537,331
glpR
BNILDI_02785: glpG - rhomboid family intramembrane serine protease GlpG, at 537,348 to 538,178
glpG
Position (kb) 534
535
536
537 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 534.040 kb on + strand, within rtcB at 534.044 kb on - strand, within rtcB at 534.111 kb on - strand, within rtcB at 534.138 kb on + strand, within rtcB at 534.212 kb on + strand, within rtcB at 534.212 kb on + strand, within rtcB at 534.212 kb on + strand, within rtcB at 534.212 kb on + strand, within rtcB at 534.212 kb on + strand, within rtcB at 534.212 kb on + strand, within rtcB at 534.213 kb on - strand, within rtcB at 534.213 kb on - strand, within rtcB at 534.213 kb on - strand, within rtcB at 534.213 kb on - strand, within rtcB at 534.213 kb on - strand, within rtcB at 534.335 kb on + strand, within rtcB at 534.335 kb on + strand, within rtcB at 534.335 kb on + strand, within rtcB at 534.335 kb on + strand, within rtcB at 534.335 kb on + strand, within rtcB at 534.336 kb on - strand, within rtcB at 534.336 kb on - strand, within rtcB at 534.336 kb on - strand, within rtcB at 534.342 kb on + strand, within rtcB at 534.342 kb on + strand, within rtcB at 534.630 kb on + strand, within rtcB at 534.658 kb on + strand, within rtcB at 534.678 kb on + strand, within rtcB at 534.679 kb on - strand, within rtcB at 534.711 kb on + strand at 534.712 kb on - strand at 534.797 kb on + strand at 534.840 kb on - strand at 534.977 kb on + strand at 534.985 kb on + strand at 534.985 kb on + strand at 534.985 kb on + strand at 534.989 kb on + strand at 534.989 kb on + strand at 534.990 kb on - strand at 534.999 kb on - strand at 535.091 kb on - strand at 535.170 kb on - strand, within rtcR at 535.170 kb on - strand, within rtcR at 535.257 kb on + strand, within rtcR at 535.257 kb on + strand, within rtcR at 535.314 kb on + strand, within rtcR at 535.315 kb on - strand, within rtcR at 535.318 kb on + strand, within rtcR at 535.318 kb on + strand, within rtcR at 535.402 kb on + strand, within rtcR at 535.403 kb on - strand, within rtcR at 535.483 kb on - strand, within rtcR at 535.484 kb on + strand, within rtcR at 535.484 kb on + strand, within rtcR at 535.706 kb on + strand, within rtcR at 535.707 kb on - strand, within rtcR at 535.823 kb on - strand, within rtcR at 536.034 kb on + strand, within rtcR at 536.112 kb on + strand, within rtcR at 536.113 kb on - strand, within rtcR at 536.160 kb on - strand, within rtcR at 536.311 kb on + strand, within rtcR at 536.327 kb on + strand, within rtcR at 536.425 kb on + strand, within rtcR at 536.425 kb on + strand, within rtcR at 536.594 kb on - strand at 536.657 kb on + strand, within glpR at 536.658 kb on - strand, within glpR at 536.658 kb on - strand, within glpR at 536.658 kb on - strand, within glpR at 536.658 kb on - strand, within glpR at 536.658 kb on - strand, within glpR at 536.678 kb on - strand, within glpR at 536.726 kb on - strand, within glpR at 536.998 kb on + strand, within glpR at 537.276 kb on + strand at 537.277 kb on - strand at 537.277 kb on - strand at 537.305 kb on - strand at 537.333 kb on + strand at 537.334 kb on - strand at 537.393 kb on + strand at 537.488 kb on + strand, within glpG at 537.488 kb on + strand, within glpG at 537.488 kb on + strand, within glpG at 537.489 kb on - strand, within glpG at 537.566 kb on - strand, within glpG at 537.566 kb on - strand, within glpG at 537.566 kb on - strand, within glpG at 537.566 kb on - strand, within glpG
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 534,040 + rtcB BNILDI_02770 0.38 -0.1 534,044 - rtcB BNILDI_02770 0.38 -1.8 534,111 - rtcB BNILDI_02770 0.43 +0.9 534,138 + rtcB BNILDI_02770 0.46 +0.6 534,212 + rtcB BNILDI_02770 0.52 +0.3 534,212 + rtcB BNILDI_02770 0.52 -0.5 534,212 + rtcB BNILDI_02770 0.52 -0.3 534,212 + rtcB BNILDI_02770 0.52 +0.9 534,212 + rtcB BNILDI_02770 0.52 -0.1 534,212 + rtcB BNILDI_02770 0.52 +1.6 534,213 - rtcB BNILDI_02770 0.52 -0.3 534,213 - rtcB BNILDI_02770 0.52 -1.8 534,213 - rtcB BNILDI_02770 0.52 -2.8 534,213 - rtcB BNILDI_02770 0.52 -0.0 534,213 - rtcB BNILDI_02770 0.52 +1.9 534,335 + rtcB BNILDI_02770 0.62 +0.8 534,335 + rtcB BNILDI_02770 0.62 -0.6 534,335 + rtcB BNILDI_02770 0.62 -1.2 534,335 + rtcB BNILDI_02770 0.62 -1.5 534,335 + rtcB BNILDI_02770 0.62 +1.0 534,336 - rtcB BNILDI_02770 0.62 -3.2 534,336 - rtcB BNILDI_02770 0.62 -0.4 534,336 - rtcB BNILDI_02770 0.62 -0.7 534,342 + rtcB BNILDI_02770 0.62 +0.6 534,342 + rtcB BNILDI_02770 0.62 -1.4 534,630 + rtcB BNILDI_02770 0.86 +0.1 534,658 + rtcB BNILDI_02770 0.88 +1.3 534,678 + rtcB BNILDI_02770 0.90 +1.3 534,679 - rtcB BNILDI_02770 0.90 -0.5 534,711 + +0.6 534,712 - +0.4 534,797 + +0.3 534,840 - -0.7 534,977 + -2.3 534,985 + +0.0 534,985 + -0.6 534,985 + +2.8 534,989 + +1.4 534,989 + -0.6 534,990 - +0.1 534,999 - -0.5 535,091 - -2.8 535,170 - rtcR BNILDI_02775 0.11 +0.6 535,170 - rtcR BNILDI_02775 0.11 +0.7 535,257 + rtcR BNILDI_02775 0.17 -3.2 535,257 + rtcR BNILDI_02775 0.17 +0.6 535,314 + rtcR BNILDI_02775 0.20 +1.6 535,315 - rtcR BNILDI_02775 0.20 -0.4 535,318 + rtcR BNILDI_02775 0.20 +0.3 535,318 + rtcR BNILDI_02775 0.20 -0.1 535,402 + rtcR BNILDI_02775 0.26 +0.2 535,403 - rtcR BNILDI_02775 0.26 -2.0 535,483 - rtcR BNILDI_02775 0.31 +0.1 535,484 + rtcR BNILDI_02775 0.31 -1.8 535,484 + rtcR BNILDI_02775 0.31 -0.1 535,706 + rtcR BNILDI_02775 0.45 +0.7 535,707 - rtcR BNILDI_02775 0.45 +3.1 535,823 - rtcR BNILDI_02775 0.52 -0.3 536,034 + rtcR BNILDI_02775 0.65 +0.7 536,112 + rtcR BNILDI_02775 0.70 +2.3 536,113 - rtcR BNILDI_02775 0.70 +1.7 536,160 - rtcR BNILDI_02775 0.73 +0.4 536,311 + rtcR BNILDI_02775 0.82 -0.1 536,327 + rtcR BNILDI_02775 0.83 -1.7 536,425 + rtcR BNILDI_02775 0.90 +0.5 536,425 + rtcR BNILDI_02775 0.90 -2.1 536,594 - +0.1 536,657 + glpR BNILDI_02780 0.11 -0.3 536,658 - glpR BNILDI_02780 0.11 -1.1 536,658 - glpR BNILDI_02780 0.11 +0.1 536,658 - glpR BNILDI_02780 0.11 -1.1 536,658 - glpR BNILDI_02780 0.11 -0.9 536,658 - glpR BNILDI_02780 0.11 -0.9 536,678 - glpR BNILDI_02780 0.14 -0.6 536,726 - glpR BNILDI_02780 0.20 -0.3 536,998 + glpR BNILDI_02780 0.56 -3.8 537,276 + -0.6 537,277 - -0.5 537,277 - -1.2 537,305 - -0.3 537,333 + -2.4 537,334 - -0.1 537,393 + +1.0 537,488 + glpG BNILDI_02785 0.17 -2.8 537,488 + glpG BNILDI_02785 0.17 +1.8 537,488 + glpG BNILDI_02785 0.17 -1.0 537,489 - glpG BNILDI_02785 0.17 -0.5 537,566 - glpG BNILDI_02785 0.26 +0.4 537,566 - glpG BNILDI_02785 0.26 +0.2 537,566 - glpG BNILDI_02785 0.26 -0.0 537,566 - glpG BNILDI_02785 0.26 +1.2
Or see this region's nucleotide sequence