Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pabA and fic are separated by 31 nucleotides fic and BNILDI_02510 overlap by 11 nucleotides BNILDI_02510 and ppiA are separated by 104 nucleotides ppiA and tsgA are separated by 270 nucleotides
BNILDI_02500: pabA - aminodeoxychorismate synthase component 2, at 472,237 to 472,800
pabA
BNILDI_02505: fic - putative adenosine monophosphate-protein transferase Fic, at 472,832 to 473,434
fic
BNILDI_02510: BNILDI_02510 - DUF2559 domain-containing protein, at 473,424 to 473,591
_02510
BNILDI_02515: ppiA - peptidylprolyl isomerase A, at 473,696 to 474,268
ppiA
BNILDI_02520: tsgA - MFS transporter TsgA, at 474,539 to 475,720
tsgA
Position (kb) 473
474 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 472.434 kb on + strand, within pabA at 472.459 kb on + strand, within pabA at 472.474 kb on - strand, within pabA at 472.639 kb on - strand, within pabA at 472.674 kb on + strand, within pabA at 472.674 kb on + strand, within pabA at 472.717 kb on - strand, within pabA at 472.749 kb on - strand at 472.755 kb on - strand at 472.778 kb on + strand at 472.778 kb on + strand at 472.782 kb on + strand at 472.782 kb on + strand at 472.786 kb on + strand at 472.786 kb on + strand at 472.786 kb on + strand at 472.787 kb on - strand at 472.787 kb on - strand at 472.787 kb on - strand at 472.828 kb on + strand at 472.834 kb on - strand at 472.834 kb on - strand at 472.867 kb on + strand at 472.871 kb on + strand at 472.871 kb on + strand at 472.872 kb on - strand at 472.872 kb on - strand at 473.052 kb on + strand, within fic at 473.154 kb on + strand, within fic at 473.154 kb on + strand, within fic at 473.154 kb on + strand, within fic at 473.155 kb on - strand, within fic at 473.155 kb on - strand, within fic at 473.155 kb on - strand, within fic at 473.381 kb on - strand at 473.400 kb on + strand at 473.435 kb on - strand at 473.540 kb on + strand, within BNILDI_02510 at 473.627 kb on + strand at 473.627 kb on + strand at 473.627 kb on + strand at 473.628 kb on - strand at 473.726 kb on + strand at 473.727 kb on - strand at 474.037 kb on + strand, within ppiA at 474.038 kb on - strand, within ppiA at 474.065 kb on - strand, within ppiA at 474.073 kb on + strand, within ppiA at 474.073 kb on + strand, within ppiA at 474.073 kb on + strand, within ppiA at 474.076 kb on - strand, within ppiA at 474.154 kb on + strand, within ppiA at 474.317 kb on + strand at 474.317 kb on + strand at 474.322 kb on - strand at 474.373 kb on + strand at 474.373 kb on + strand at 474.457 kb on + strand at 474.588 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 472,434 + pabA BNILDI_02500 0.35 -0.2 472,459 + pabA BNILDI_02500 0.39 -1.8 472,474 - pabA BNILDI_02500 0.42 +1.5 472,639 - pabA BNILDI_02500 0.71 +0.4 472,674 + pabA BNILDI_02500 0.77 +0.1 472,674 + pabA BNILDI_02500 0.77 -2.2 472,717 - pabA BNILDI_02500 0.85 -1.0 472,749 - -0.3 472,755 - -0.6 472,778 + +0.5 472,778 + -1.2 472,782 + +1.4 472,782 + -0.8 472,786 + -0.5 472,786 + -0.5 472,786 + -0.1 472,787 - -0.9 472,787 - -1.2 472,787 - -3.7 472,828 + +0.1 472,834 - -2.6 472,834 - +0.5 472,867 + -0.7 472,871 + -1.3 472,871 + -0.7 472,872 - -0.2 472,872 - +0.3 473,052 + fic BNILDI_02505 0.36 +1.1 473,154 + fic BNILDI_02505 0.53 -0.8 473,154 + fic BNILDI_02505 0.53 +1.6 473,154 + fic BNILDI_02505 0.53 -0.1 473,155 - fic BNILDI_02505 0.54 +0.2 473,155 - fic BNILDI_02505 0.54 -0.6 473,155 - fic BNILDI_02505 0.54 -0.8 473,381 - +0.2 473,400 + +0.3 473,435 - +0.5 473,540 + BNILDI_02510 0.69 -0.9 473,627 + -0.8 473,627 + +0.8 473,627 + +0.7 473,628 - +0.2 473,726 + +2.1 473,727 - +0.3 474,037 + ppiA BNILDI_02515 0.60 -0.2 474,038 - ppiA BNILDI_02515 0.60 -0.4 474,065 - ppiA BNILDI_02515 0.64 +0.1 474,073 + ppiA BNILDI_02515 0.66 -0.4 474,073 + ppiA BNILDI_02515 0.66 -0.2 474,073 + ppiA BNILDI_02515 0.66 -0.8 474,076 - ppiA BNILDI_02515 0.66 -3.4 474,154 + ppiA BNILDI_02515 0.80 -0.5 474,317 + +1.0 474,317 + +1.3 474,322 - -0.2 474,373 + +0.0 474,373 + +0.4 474,457 + +0.5 474,588 - -1.8
Or see this region's nucleotide sequence