Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lptA and lptB are separated by 6 nucleotides lptB and rpoN are separated by 47 nucleotides rpoN and BNILDI_01800 are separated by 22 nucleotides BNILDI_01800 and ptsN are separated by 117 nucleotides ptsN and rapZ are separated by 45 nucleotides
BNILDI_01785: lptA - lipopolysaccharide ABC transporter substrate-binding protein LptA, at 347,754 to 348,311
lptA
BNILDI_01790: lptB - LPS export ABC transporter ATP-binding protein, at 348,318 to 349,043
lptB
BNILDI_01795: rpoN - RNA polymerase factor sigma-54, at 349,091 to 350,524
rpoN
BNILDI_01800: BNILDI_01800 - Ribosome hibernation promoting factor, at 350,547 to 350,834
_01800
BNILDI_01805: ptsN - PTS IIA-like nitrogen regulatory protein PtsN, at 350,952 to 351,443
ptsN
BNILDI_01810: rapZ - RNase adapter RapZ, at 351,489 to 352,343
rapZ
Position (kb) 349
350
351 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 350.510 kb on - strand at 350.634 kb on + strand, within BNILDI_01800 at 350.658 kb on + strand, within BNILDI_01800 at 350.658 kb on + strand, within BNILDI_01800 at 350.658 kb on + strand, within BNILDI_01800 at 350.659 kb on - strand, within BNILDI_01800 at 350.659 kb on - strand, within BNILDI_01800 at 350.659 kb on - strand, within BNILDI_01800 at 350.659 kb on - strand, within BNILDI_01800 at 350.757 kb on + strand, within BNILDI_01800 at 350.757 kb on + strand, within BNILDI_01800 at 350.757 kb on + strand, within BNILDI_01800 at 350.758 kb on - strand, within BNILDI_01800 at 350.758 kb on - strand, within BNILDI_01800 at 350.758 kb on - strand, within BNILDI_01800 at 350.758 kb on - strand, within BNILDI_01800 at 350.758 kb on - strand, within BNILDI_01800 at 350.776 kb on + strand, within BNILDI_01800 at 350.776 kb on + strand, within BNILDI_01800 at 350.811 kb on - strand at 350.848 kb on + strand at 350.848 kb on + strand at 350.893 kb on + strand at 350.941 kb on + strand at 351.081 kb on - strand, within ptsN at 351.110 kb on + strand, within ptsN at 351.110 kb on + strand, within ptsN at 351.156 kb on - strand, within ptsN at 351.188 kb on + strand, within ptsN at 351.189 kb on - strand, within ptsN at 351.232 kb on + strand, within ptsN at 351.232 kb on + strand, within ptsN at 351.232 kb on + strand, within ptsN at 351.302 kb on + strand, within ptsN at 351.317 kb on + strand, within ptsN at 351.317 kb on + strand, within ptsN at 351.400 kb on - strand at 351.425 kb on + strand at 351.425 kb on + strand at 351.441 kb on + strand at 351.442 kb on - strand at 351.442 kb on - strand at 351.465 kb on - strand at 351.486 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 350,510 - -1.4 350,634 + BNILDI_01800 0.30 +1.1 350,658 + BNILDI_01800 0.39 +0.0 350,658 + BNILDI_01800 0.39 -0.9 350,658 + BNILDI_01800 0.39 -0.5 350,659 - BNILDI_01800 0.39 -1.9 350,659 - BNILDI_01800 0.39 -2.7 350,659 - BNILDI_01800 0.39 -0.7 350,659 - BNILDI_01800 0.39 -0.9 350,757 + BNILDI_01800 0.73 -0.2 350,757 + BNILDI_01800 0.73 +0.1 350,757 + BNILDI_01800 0.73 -3.0 350,758 - BNILDI_01800 0.73 +0.2 350,758 - BNILDI_01800 0.73 -1.8 350,758 - BNILDI_01800 0.73 -0.7 350,758 - BNILDI_01800 0.73 -3.5 350,758 - BNILDI_01800 0.73 -0.0 350,776 + BNILDI_01800 0.80 +0.8 350,776 + BNILDI_01800 0.80 +1.3 350,811 - -1.6 350,848 + -0.1 350,848 + +0.6 350,893 + +0.4 350,941 + +0.4 351,081 - ptsN BNILDI_01805 0.26 -4.0 351,110 + ptsN BNILDI_01805 0.32 -0.9 351,110 + ptsN BNILDI_01805 0.32 -0.8 351,156 - ptsN BNILDI_01805 0.41 +0.6 351,188 + ptsN BNILDI_01805 0.48 -1.1 351,189 - ptsN BNILDI_01805 0.48 +1.9 351,232 + ptsN BNILDI_01805 0.57 -4.0 351,232 + ptsN BNILDI_01805 0.57 -0.1 351,232 + ptsN BNILDI_01805 0.57 -3.3 351,302 + ptsN BNILDI_01805 0.71 -0.1 351,317 + ptsN BNILDI_01805 0.74 +0.3 351,317 + ptsN BNILDI_01805 0.74 -0.2 351,400 - -2.1 351,425 + -3.6 351,425 + -2.6 351,441 + +0.1 351,442 - +1.4 351,442 - -2.1 351,465 - +2.6 351,486 + -0.3
Or see this region's nucleotide sequence