Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt murA_D396N and ibaG are separated by 54 nucleotides ibaG and mlaC are separated by 144 nucleotides mlaC and mlaC overlap by 1 nucleotides mlaC and mlaD are separated by 18 nucleotides
BNILDI_01730: murA_D396N - UDP-N-acetylglucosamine 1-carboxyvinyltransferase MurA, at 339,609 to 340,868
_D396N
BNILDI_01735: ibaG - BolA family iron metabolism protein IbaG, at 340,923 to 341,192
ibaG
BNILDI_01740: mlaC - phospholipid-binding protein MlaC, at 341,337 to 341,630
mlaC
BNILDI_01745: mlaC - phospholipid-binding protein MlaC, at 341,630 to 342,265
mlaC
BNILDI_01750: mlaD - outer membrane lipid asymmetry maintenance protein MlaD, at 342,284 to 342,835
mlaD
Position (kb) 341
342 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 340.968 kb on - strand, within ibaG at 340.970 kb on - strand, within ibaG at 340.970 kb on - strand, within ibaG at 340.970 kb on - strand, within ibaG at 341.243 kb on + strand at 341.243 kb on + strand at 341.244 kb on - strand at 341.366 kb on + strand at 341.366 kb on + strand at 341.387 kb on - strand, within mlaC at 341.513 kb on + strand, within mlaC at 341.513 kb on + strand, within mlaC at 341.749 kb on + strand, within mlaC at 341.807 kb on - strand, within mlaC at 341.854 kb on + strand, within mlaC at 341.931 kb on + strand, within mlaC at 341.931 kb on + strand, within mlaC at 341.932 kb on - strand, within mlaC at 341.932 kb on - strand, within mlaC at 341.932 kb on - strand, within mlaC at 341.967 kb on + strand, within mlaC at 342.024 kb on + strand, within mlaC at 342.024 kb on + strand, within mlaC at 342.062 kb on + strand, within mlaC at 342.062 kb on + strand, within mlaC at 342.062 kb on + strand, within mlaC at 342.062 kb on + strand, within mlaC at 342.062 kb on + strand, within mlaC at 342.063 kb on - strand, within mlaC at 342.066 kb on + strand, within mlaC at 342.067 kb on - strand, within mlaC at 342.102 kb on + strand, within mlaC at 342.181 kb on + strand, within mlaC at 342.285 kb on + strand at 342.322 kb on + strand at 342.352 kb on + strand, within mlaD at 342.353 kb on - strand, within mlaD at 342.353 kb on - strand, within mlaD at 342.358 kb on + strand, within mlaD at 342.358 kb on + strand, within mlaD at 342.359 kb on - strand, within mlaD at 342.424 kb on + strand, within mlaD at 342.424 kb on + strand, within mlaD at 342.425 kb on - strand, within mlaD at 342.425 kb on - strand, within mlaD at 342.425 kb on - strand, within mlaD at 342.439 kb on + strand, within mlaD at 342.504 kb on + strand, within mlaD at 342.505 kb on - strand, within mlaD at 342.505 kb on - strand, within mlaD at 342.530 kb on - strand, within mlaD at 342.551 kb on - strand, within mlaD at 342.568 kb on - strand, within mlaD at 342.603 kb on + strand, within mlaD at 342.604 kb on - strand, within mlaD at 342.623 kb on - strand, within mlaD
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 340,968 - ibaG BNILDI_01735 0.17 +0.2 340,970 - ibaG BNILDI_01735 0.17 -0.6 340,970 - ibaG BNILDI_01735 0.17 -0.7 340,970 - ibaG BNILDI_01735 0.17 +1.4 341,243 + -0.2 341,243 + -0.3 341,244 - -0.4 341,366 + +1.7 341,366 + -0.8 341,387 - mlaC BNILDI_01740 0.17 -1.1 341,513 + mlaC BNILDI_01740 0.60 -2.7 341,513 + mlaC BNILDI_01740 0.60 -0.4 341,749 + mlaC BNILDI_01745 0.19 -0.8 341,807 - mlaC BNILDI_01745 0.28 +0.7 341,854 + mlaC BNILDI_01745 0.35 -0.5 341,931 + mlaC BNILDI_01745 0.47 -0.5 341,931 + mlaC BNILDI_01745 0.47 +0.1 341,932 - mlaC BNILDI_01745 0.47 +0.9 341,932 - mlaC BNILDI_01745 0.47 -0.8 341,932 - mlaC BNILDI_01745 0.47 +1.0 341,967 + mlaC BNILDI_01745 0.53 -1.1 342,024 + mlaC BNILDI_01745 0.62 -2.5 342,024 + mlaC BNILDI_01745 0.62 -1.6 342,062 + mlaC BNILDI_01745 0.68 -0.1 342,062 + mlaC BNILDI_01745 0.68 -0.9 342,062 + mlaC BNILDI_01745 0.68 +1.1 342,062 + mlaC BNILDI_01745 0.68 -2.2 342,062 + mlaC BNILDI_01745 0.68 +0.5 342,063 - mlaC BNILDI_01745 0.68 +0.8 342,066 + mlaC BNILDI_01745 0.69 +0.1 342,067 - mlaC BNILDI_01745 0.69 -0.8 342,102 + mlaC BNILDI_01745 0.74 +0.4 342,181 + mlaC BNILDI_01745 0.87 +1.5 342,285 + -1.0 342,322 + -0.0 342,352 + mlaD BNILDI_01750 0.12 -0.5 342,353 - mlaD BNILDI_01750 0.12 -0.1 342,353 - mlaD BNILDI_01750 0.12 -0.4 342,358 + mlaD BNILDI_01750 0.13 +2.7 342,358 + mlaD BNILDI_01750 0.13 +0.1 342,359 - mlaD BNILDI_01750 0.14 +1.6 342,424 + mlaD BNILDI_01750 0.25 -0.3 342,424 + mlaD BNILDI_01750 0.25 -3.0 342,425 - mlaD BNILDI_01750 0.26 -0.1 342,425 - mlaD BNILDI_01750 0.26 -0.4 342,425 - mlaD BNILDI_01750 0.26 -0.7 342,439 + mlaD BNILDI_01750 0.28 -3.3 342,504 + mlaD BNILDI_01750 0.40 -0.1 342,505 - mlaD BNILDI_01750 0.40 +0.5 342,505 - mlaD BNILDI_01750 0.40 -0.5 342,530 - mlaD BNILDI_01750 0.45 -0.1 342,551 - mlaD BNILDI_01750 0.48 -2.6 342,568 - mlaD BNILDI_01750 0.51 -0.3 342,603 + mlaD BNILDI_01750 0.58 +1.5 342,604 - mlaD BNILDI_01750 0.58 -0.2 342,623 - mlaD BNILDI_01750 0.61 -1.4
Or see this region's nucleotide sequence