Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nlpI and pnp are separated by 108 nucleotides pnp and rpsO are separated by 246 nucleotides rpsO and truB are separated by 148 nucleotides
BNILDI_01590: nlpI - lipoprotein NlpI, at 312,561 to 313,445
nlpI
BNILDI_01595: pnp - polyribonucleotide nucleotidyltransferase, at 313,554 to 315,689
pnp
BNILDI_01605: rpsO - 30S ribosomal protein S15, at 315,936 to 316,205
rpsO
BNILDI_01610: truB - tRNA pseudouridine(55) synthase TruB, at 316,354 to 317,298
truB
Position (kb) 313
314
315
316 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 312.554 kb on - strand at 312.563 kb on - strand at 312.563 kb on - strand at 312.711 kb on + strand, within nlpI at 312.712 kb on - strand, within nlpI at 312.712 kb on - strand, within nlpI at 312.716 kb on - strand, within nlpI at 312.719 kb on - strand, within nlpI at 312.719 kb on - strand, within nlpI at 312.719 kb on - strand, within nlpI at 312.719 kb on - strand, within nlpI at 312.730 kb on + strand, within nlpI at 312.731 kb on - strand, within nlpI at 312.731 kb on - strand, within nlpI at 312.829 kb on + strand, within nlpI at 312.829 kb on + strand, within nlpI at 313.055 kb on - strand, within nlpI at 313.060 kb on + strand, within nlpI at 313.133 kb on - strand, within nlpI at 313.135 kb on - strand, within nlpI at 313.163 kb on + strand, within nlpI at 313.232 kb on - strand, within nlpI at 313.232 kb on - strand, within nlpI at 313.232 kb on - strand, within nlpI at 313.232 kb on - strand, within nlpI at 313.250 kb on - strand, within nlpI at 313.307 kb on + strand, within nlpI at 313.307 kb on + strand, within nlpI at 313.307 kb on + strand, within nlpI at 313.308 kb on - strand, within nlpI at 313.382 kb on + strand at 313.382 kb on + strand at 313.403 kb on + strand at 313.404 kb on - strand at 313.555 kb on + strand at 313.623 kb on - strand at 313.623 kb on - strand at 313.808 kb on + strand, within pnp at 313.866 kb on - strand, within pnp at 313.866 kb on - strand, within pnp at 313.911 kb on - strand, within pnp at 313.935 kb on - strand, within pnp at 314.186 kb on + strand, within pnp at 314.260 kb on - strand, within pnp at 314.266 kb on + strand, within pnp at 314.267 kb on - strand, within pnp at 314.267 kb on - strand, within pnp at 314.267 kb on - strand, within pnp at 314.373 kb on - strand, within pnp at 314.410 kb on - strand, within pnp at 314.415 kb on + strand, within pnp at 314.573 kb on + strand, within pnp at 314.700 kb on - strand, within pnp at 314.723 kb on + strand, within pnp at 314.763 kb on + strand, within pnp at 315.224 kb on - strand, within pnp at 315.590 kb on + strand at 315.596 kb on + strand at 315.693 kb on - strand at 315.693 kb on - strand at 315.768 kb on + strand at 315.791 kb on + strand at 315.792 kb on - strand at 315.792 kb on - strand at 315.792 kb on - strand at 315.848 kb on - strand at 315.856 kb on + strand at 315.857 kb on - strand at 315.857 kb on - strand at 316.485 kb on + strand, within truB at 316.485 kb on + strand, within truB at 316.485 kb on + strand, within truB at 316.680 kb on + strand, within truB at 316.681 kb on - strand, within truB at 316.681 kb on - strand, within truB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 312,554 - +0.0 312,563 - +0.1 312,563 - -0.5 312,711 + nlpI BNILDI_01590 0.17 +0.5 312,712 - nlpI BNILDI_01590 0.17 -0.6 312,712 - nlpI BNILDI_01590 0.17 +0.9 312,716 - nlpI BNILDI_01590 0.18 +0.2 312,719 - nlpI BNILDI_01590 0.18 +2.1 312,719 - nlpI BNILDI_01590 0.18 -0.1 312,719 - nlpI BNILDI_01590 0.18 -0.2 312,719 - nlpI BNILDI_01590 0.18 +0.3 312,730 + nlpI BNILDI_01590 0.19 +1.4 312,731 - nlpI BNILDI_01590 0.19 +0.2 312,731 - nlpI BNILDI_01590 0.19 +1.8 312,829 + nlpI BNILDI_01590 0.30 -0.7 312,829 + nlpI BNILDI_01590 0.30 -0.5 313,055 - nlpI BNILDI_01590 0.56 -0.4 313,060 + nlpI BNILDI_01590 0.56 -1.5 313,133 - nlpI BNILDI_01590 0.65 -3.4 313,135 - nlpI BNILDI_01590 0.65 -2.8 313,163 + nlpI BNILDI_01590 0.68 -1.7 313,232 - nlpI BNILDI_01590 0.76 -0.4 313,232 - nlpI BNILDI_01590 0.76 +0.0 313,232 - nlpI BNILDI_01590 0.76 -3.1 313,232 - nlpI BNILDI_01590 0.76 -0.1 313,250 - nlpI BNILDI_01590 0.78 -2.6 313,307 + nlpI BNILDI_01590 0.84 +0.5 313,307 + nlpI BNILDI_01590 0.84 +0.4 313,307 + nlpI BNILDI_01590 0.84 +1.0 313,308 - nlpI BNILDI_01590 0.84 -0.2 313,382 + -2.6 313,382 + -1.2 313,403 + -0.1 313,404 - -0.2 313,555 + -1.2 313,623 - +0.3 313,623 - -1.1 313,808 + pnp BNILDI_01595 0.12 -2.8 313,866 - pnp BNILDI_01595 0.15 +0.0 313,866 - pnp BNILDI_01595 0.15 -1.7 313,911 - pnp BNILDI_01595 0.17 -2.4 313,935 - pnp BNILDI_01595 0.18 -1.0 314,186 + pnp BNILDI_01595 0.30 -2.1 314,260 - pnp BNILDI_01595 0.33 -3.4 314,266 + pnp BNILDI_01595 0.33 -1.1 314,267 - pnp BNILDI_01595 0.33 -0.8 314,267 - pnp BNILDI_01595 0.33 -1.7 314,267 - pnp BNILDI_01595 0.33 -4.7 314,373 - pnp BNILDI_01595 0.38 -2.7 314,410 - pnp BNILDI_01595 0.40 -0.8 314,415 + pnp BNILDI_01595 0.40 -0.9 314,573 + pnp BNILDI_01595 0.48 +1.2 314,700 - pnp BNILDI_01595 0.54 -1.1 314,723 + pnp BNILDI_01595 0.55 +0.9 314,763 + pnp BNILDI_01595 0.57 -2.8 315,224 - pnp BNILDI_01595 0.78 -0.9 315,590 + -2.4 315,596 + -0.8 315,693 - -2.2 315,693 - +1.1 315,768 + -2.0 315,791 + -1.7 315,792 - -1.0 315,792 - -2.1 315,792 - -0.4 315,848 - -0.2 315,856 + -1.1 315,857 - -0.6 315,857 - +0.4 316,485 + truB BNILDI_01610 0.14 -0.9 316,485 + truB BNILDI_01610 0.14 -0.1 316,485 + truB BNILDI_01610 0.14 -0.3 316,680 + truB BNILDI_01610 0.34 +0.9 316,681 - truB BNILDI_01610 0.35 -1.6 316,681 - truB BNILDI_01610 0.35 +1.5
Or see this region's nucleotide sequence