Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yraR and yhbO are separated by 127 nucleotides yhbO and BNILDI_01535 are separated by 153 nucleotides BNILDI_01535 and yhbQ are separated by 50 nucleotides yhbQ and yhbS overlap by 14 nucleotides yhbS and ubiT overlap by 7 nucleotides
BNILDI_01525: yraR - Putative NAD(P)-binding protein YraR, at 301,328 to 301,963
yraR
BNILDI_01530: yhbO - protein/nucleic acid deglycase, at 302,091 to 302,609
yhbO
BNILDI_01535: BNILDI_01535 - UPF0306 protein, at 302,763 to 303,032
_01535
BNILDI_01540: yhbQ - DNA damage response exodeoxyribonuclease YhbQ, at 303,083 to 303,385
yhbQ
BNILDI_01545: yhbS - Uncharacterized N-acetyltransferase YhbS, at 303,372 to 303,875
yhbS
BNILDI_01550: ubiT - Ubiquinone biosynthesis accessory factor UbiT, at 303,869 to 304,393
ubiT
Position (kb) 302
303
304 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 301.829 kb on - strand, within yraR at 301.953 kb on - strand at 302.020 kb on - strand at 302.020 kb on - strand at 302.034 kb on + strand at 302.035 kb on - strand at 302.097 kb on - strand at 302.144 kb on + strand, within yhbO at 302.144 kb on + strand, within yhbO at 302.325 kb on + strand, within yhbO at 302.357 kb on + strand, within yhbO at 302.732 kb on - strand at 302.786 kb on + strand at 302.806 kb on - strand, within BNILDI_01535 at 302.902 kb on + strand, within BNILDI_01535 at 303.047 kb on - strand at 303.047 kb on - strand at 303.048 kb on + strand at 303.048 kb on + strand at 303.048 kb on + strand at 303.049 kb on - strand at 303.065 kb on + strand at 303.102 kb on - strand at 303.131 kb on + strand, within yhbQ at 303.132 kb on - strand, within yhbQ at 303.132 kb on - strand, within yhbQ at 303.133 kb on + strand, within yhbQ at 303.526 kb on + strand, within yhbS at 303.596 kb on - strand, within yhbS at 303.628 kb on + strand, within yhbS at 303.628 kb on + strand, within yhbS at 303.710 kb on - strand, within yhbS at 303.822 kb on + strand, within yhbS at 303.822 kb on + strand, within yhbS at 303.822 kb on + strand, within yhbS at 303.822 kb on + strand, within yhbS at 303.822 kb on + strand, within yhbS at 303.823 kb on - strand, within yhbS at 303.870 kb on + strand at 303.870 kb on + strand at 303.870 kb on + strand at 303.871 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 301,829 - yraR BNILDI_01525 0.79 -0.0 301,953 - +0.2 302,020 - +0.5 302,020 - -1.4 302,034 + -1.8 302,035 - -3.0 302,097 - -0.9 302,144 + yhbO BNILDI_01530 0.10 +3.1 302,144 + yhbO BNILDI_01530 0.10 +0.1 302,325 + yhbO BNILDI_01530 0.45 +0.3 302,357 + yhbO BNILDI_01530 0.51 -0.5 302,732 - -0.0 302,786 + +0.9 302,806 - BNILDI_01535 0.16 -4.2 302,902 + BNILDI_01535 0.51 -0.6 303,047 - -0.7 303,047 - -0.6 303,048 + +0.2 303,048 + +0.1 303,048 + -1.9 303,049 - -0.2 303,065 + +0.6 303,102 - -3.0 303,131 + yhbQ BNILDI_01540 0.16 +0.9 303,132 - yhbQ BNILDI_01540 0.16 -0.5 303,132 - yhbQ BNILDI_01540 0.16 -0.5 303,133 + yhbQ BNILDI_01540 0.17 -0.1 303,526 + yhbS BNILDI_01545 0.31 +0.3 303,596 - yhbS BNILDI_01545 0.44 -1.8 303,628 + yhbS BNILDI_01545 0.51 -0.4 303,628 + yhbS BNILDI_01545 0.51 +1.2 303,710 - yhbS BNILDI_01545 0.67 -1.4 303,822 + yhbS BNILDI_01545 0.89 -1.2 303,822 + yhbS BNILDI_01545 0.89 -3.5 303,822 + yhbS BNILDI_01545 0.89 -1.0 303,822 + yhbS BNILDI_01545 0.89 +0.8 303,822 + yhbS BNILDI_01545 0.89 -0.4 303,823 - yhbS BNILDI_01545 0.89 -1.1 303,870 + -0.2 303,870 + -0.4 303,870 + -1.5 303,871 - -1.1
Or see this region's nucleotide sequence