Strain Fitness in Escherichia coli ECRC62 around BNILDI_01400

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgarD and prlF are separated by 148 nucleotidesprlF and yhaV overlap by 1 nucleotidesyhaV and agaR are separated by 54 nucleotides BNILDI_01395: garD - galactarate dehydratase, at 276,302 to 277,873 garD BNILDI_01400: prlF - type II toxin-antitoxin system antitoxin PrlF, at 278,022 to 278,357 prlF BNILDI_01405: yhaV - type II toxin-antitoxin system ribonuclease toxin YhaV, at 278,357 to 278,821 yhaV BNILDI_01410: agaR - aga operon transcriptional regulator AgaR, at 278,876 to 279,685 agaR Position (kb) 278 279Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 277.214 kb on + strand, within garDat 277.228 kb on - strand, within garDat 277.364 kb on + strand, within garDat 277.365 kb on - strand, within garDat 277.701 kb on + strand, within garDat 277.702 kb on - strand, within garDat 277.909 kb on - strandat 277.950 kb on + strandat 277.981 kb on - strandat 278.006 kb on - strandat 278.031 kb on - strandat 278.213 kb on + strand, within prlFat 278.214 kb on - strand, within prlFat 278.214 kb on - strand, within prlFat 278.216 kb on + strand, within prlFat 278.216 kb on + strand, within prlFat 278.216 kb on + strand, within prlFat 278.217 kb on - strand, within prlFat 278.393 kb on + strandat 278.394 kb on - strandat 278.430 kb on + strand, within yhaVat 278.452 kb on - strand, within yhaVat 278.499 kb on + strand, within yhaVat 278.714 kb on + strand, within yhaVat 278.715 kb on - strand, within yhaVat 278.735 kb on + strand, within yhaVat 278.735 kb on + strand, within yhaVat 278.742 kb on + strand, within yhaVat 278.969 kb on - strand, within agaRat 278.989 kb on + strand, within agaRat 279.013 kb on + strand, within agaRat 279.089 kb on - strand, within agaRat 279.126 kb on - strand, within agaRat 279.142 kb on + strand, within agaRat 279.293 kb on + strand, within agaR

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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277,214 + garD BNILDI_01395 0.58 +1.0
277,228 - garD BNILDI_01395 0.59 -0.7
277,364 + garD BNILDI_01395 0.68 +1.7
277,365 - garD BNILDI_01395 0.68 +3.5
277,701 + garD BNILDI_01395 0.89 -1.7
277,702 - garD BNILDI_01395 0.89 +1.5
277,909 - -0.4
277,950 + -3.2
277,981 - -0.2
278,006 - -0.7
278,031 - +1.3
278,213 + prlF BNILDI_01400 0.57 -4.9
278,214 - prlF BNILDI_01400 0.57 -1.4
278,214 - prlF BNILDI_01400 0.57 -0.2
278,216 + prlF BNILDI_01400 0.58 -2.1
278,216 + prlF BNILDI_01400 0.58 -0.3
278,216 + prlF BNILDI_01400 0.58 -2.2
278,217 - prlF BNILDI_01400 0.58 -2.2
278,393 + +0.7
278,394 - +0.2
278,430 + yhaV BNILDI_01405 0.16 +1.1
278,452 - yhaV BNILDI_01405 0.20 +1.1
278,499 + yhaV BNILDI_01405 0.31 +0.6
278,714 + yhaV BNILDI_01405 0.77 +0.3
278,715 - yhaV BNILDI_01405 0.77 +0.8
278,735 + yhaV BNILDI_01405 0.81 -1.1
278,735 + yhaV BNILDI_01405 0.81 -0.3
278,742 + yhaV BNILDI_01405 0.83 +0.5
278,969 - agaR BNILDI_01410 0.11 +0.6
278,989 + agaR BNILDI_01410 0.14 +0.6
279,013 + agaR BNILDI_01410 0.17 +2.3
279,089 - agaR BNILDI_01410 0.26 -1.6
279,126 - agaR BNILDI_01410 0.31 -0.6
279,142 + agaR BNILDI_01410 0.33 -0.6
279,293 + agaR BNILDI_01410 0.51 +1.3

Or see this region's nucleotide sequence