Strain Fitness in Escherichia coli ECRC62 around BNILDI_16225

Experiment: L-Arabinose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntastB and astE overlap by 8 nucleotidesastE and spy are separated by 329 nucleotides BNILDI_16220: astB - N-succinylarginine dihydrolase, at 3,253,758 to 3,255,101 astB BNILDI_16225: astE - succinylglutamate desuccinylase, at 3,255,094 to 3,256,062 astE BNILDI_16230: spy - ATP-independent periplasmic protein-refolding chaperone Spy, at 3,256,392 to 3,256,877 spy Position (kb) 3255 3256 3257Strain fitness (log2 ratio) -2 -1 0 1 2at 3254.160 kb on - strand, within astBat 3254.664 kb on + strand, within astBat 3254.664 kb on + strand, within astBat 3254.930 kb on + strand, within astBat 3254.958 kb on + strand, within astBat 3255.032 kb on + strandat 3255.428 kb on + strand, within astEat 3255.428 kb on + strand, within astEat 3255.572 kb on + strand, within astEat 3255.572 kb on + strand, within astEat 3255.715 kb on - strand, within astEat 3255.773 kb on + strand, within astEat 3255.918 kb on + strand, within astEat 3255.987 kb on - strandat 3256.806 kb on - strand, within spyat 3256.854 kb on - strandat 3256.919 kb on - strandat 3257.004 kb on + strandat 3257.005 kb on - strandat 3257.038 kb on + strandat 3257.038 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Arabinose
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3,254,160 - astB BNILDI_16220 0.30 +0.3
3,254,664 + astB BNILDI_16220 0.67 +0.2
3,254,664 + astB BNILDI_16220 0.67 -0.4
3,254,930 + astB BNILDI_16220 0.87 -0.5
3,254,958 + astB BNILDI_16220 0.89 -1.5
3,255,032 + -2.5
3,255,428 + astE BNILDI_16225 0.34 +1.4
3,255,428 + astE BNILDI_16225 0.34 -1.7
3,255,572 + astE BNILDI_16225 0.49 +2.4
3,255,572 + astE BNILDI_16225 0.49 -2.7
3,255,715 - astE BNILDI_16225 0.64 +0.8
3,255,773 + astE BNILDI_16225 0.70 +0.2
3,255,918 + astE BNILDI_16225 0.85 -0.0
3,255,987 - -0.6
3,256,806 - spy BNILDI_16230 0.85 +0.1
3,256,854 - -1.1
3,256,919 - +1.2
3,257,004 + -0.6
3,257,005 - -1.1
3,257,038 + -0.7
3,257,038 + -0.1

Or see this region's nucleotide sequence