Experiment: L-Arabinose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metQ and metI are separated by 39 nucleotides metI and metN overlap by 8 nucleotides metN and gmhB are separated by 187 nucleotides
BNILDI_09265: metQ - methionine ABC transporter substrate-binding lipoprotein MetQ, at 1,858,979 to 1,859,794
metQ
BNILDI_09270: metI - D-methionine transport system permease protein MetI, at 1,859,834 to 1,860,487
metI
BNILDI_09275: metN - methionine ABC transporter ATP-binding protein MetN, at 1,860,480 to 1,861,511
metN
BNILDI_09280: gmhB - D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase, at 1,861,699 to 1,862,274
gmhB
Position (kb)
1860
1861
1862 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1859.524 kb on - strand, within metQ at 1859.530 kb on + strand, within metQ at 1859.626 kb on - strand, within metQ at 1859.626 kb on - strand, within metQ at 1859.626 kb on - strand, within metQ at 1859.696 kb on - strand, within metQ at 1859.696 kb on - strand, within metQ at 1859.794 kb on + strand at 1859.814 kb on + strand at 1859.819 kb on - strand at 1859.882 kb on + strand at 1859.882 kb on + strand at 1859.883 kb on - strand at 1859.883 kb on - strand at 1859.917 kb on + strand, within metI at 1859.946 kb on + strand, within metI at 1859.946 kb on + strand, within metI at 1860.245 kb on - strand, within metI at 1860.281 kb on - strand, within metI at 1860.350 kb on - strand, within metI at 1860.372 kb on + strand, within metI at 1860.373 kb on - strand, within metI at 1860.373 kb on - strand, within metI at 1860.373 kb on - strand, within metI at 1860.487 kb on + strand at 1860.487 kb on + strand at 1860.488 kb on - strand at 1860.501 kb on + strand at 1860.501 kb on + strand at 1860.501 kb on + strand at 1860.508 kb on - strand at 1860.546 kb on - strand at 1860.621 kb on - strand, within metN at 1860.623 kb on + strand, within metN at 1860.623 kb on + strand, within metN at 1860.623 kb on + strand, within metN at 1860.623 kb on + strand, within metN at 1860.623 kb on + strand, within metN at 1860.624 kb on - strand, within metN at 1860.624 kb on - strand, within metN at 1860.725 kb on + strand, within metN at 1860.792 kb on - strand, within metN at 1860.838 kb on - strand, within metN at 1860.838 kb on - strand, within metN at 1860.977 kb on + strand, within metN at 1861.029 kb on + strand, within metN at 1861.029 kb on + strand, within metN at 1861.030 kb on - strand, within metN at 1861.030 kb on - strand, within metN at 1861.030 kb on - strand, within metN at 1861.359 kb on + strand, within metN at 1861.360 kb on - strand, within metN at 1861.368 kb on + strand, within metN at 1861.368 kb on + strand, within metN at 1861.368 kb on + strand, within metN at 1861.368 kb on + strand, within metN at 1861.368 kb on + strand, within metN at 1861.369 kb on - strand, within metN at 1861.369 kb on - strand, within metN at 1861.371 kb on - strand, within metN at 1861.377 kb on - strand, within metN at 1861.521 kb on - strand at 1861.532 kb on + strand at 1861.533 kb on - strand at 1861.533 kb on - strand at 1861.533 kb on - strand at 1861.537 kb on + strand at 1861.538 kb on - strand at 1861.538 kb on - strand at 1861.625 kb on - strand at 1861.665 kb on + strand at 1861.680 kb on + strand at 1861.693 kb on + strand at 1862.066 kb on - strand, within gmhB at 1862.095 kb on - strand, within gmhB at 1862.217 kb on - strand at 1862.338 kb on - strand at 1862.506 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arabinose remove 1,859,524 - metQ BNILDI_09265 0.67 +0.4 1,859,530 + metQ BNILDI_09265 0.68 +0.7 1,859,626 - metQ BNILDI_09265 0.79 +1.3 1,859,626 - metQ BNILDI_09265 0.79 -2.9 1,859,626 - metQ BNILDI_09265 0.79 -0.6 1,859,696 - metQ BNILDI_09265 0.88 -0.3 1,859,696 - metQ BNILDI_09265 0.88 +1.0 1,859,794 + +1.7 1,859,814 + -0.1 1,859,819 - -0.2 1,859,882 + -3.5 1,859,882 + +0.6 1,859,883 - -0.1 1,859,883 - +0.7 1,859,917 + metI BNILDI_09270 0.13 +0.5 1,859,946 + metI BNILDI_09270 0.17 +0.3 1,859,946 + metI BNILDI_09270 0.17 -0.3 1,860,245 - metI BNILDI_09270 0.63 +0.6 1,860,281 - metI BNILDI_09270 0.68 +1.5 1,860,350 - metI BNILDI_09270 0.79 -0.6 1,860,372 + metI BNILDI_09270 0.82 +0.5 1,860,373 - metI BNILDI_09270 0.82 +0.2 1,860,373 - metI BNILDI_09270 0.82 +0.0 1,860,373 - metI BNILDI_09270 0.82 +0.5 1,860,487 + +0.2 1,860,487 + -0.5 1,860,488 - -0.0 1,860,501 + +0.2 1,860,501 + +0.6 1,860,501 + +0.1 1,860,508 - -2.4 1,860,546 - -0.2 1,860,621 - metN BNILDI_09275 0.14 +0.0 1,860,623 + metN BNILDI_09275 0.14 -0.1 1,860,623 + metN BNILDI_09275 0.14 +0.3 1,860,623 + metN BNILDI_09275 0.14 -3.4 1,860,623 + metN BNILDI_09275 0.14 +0.6 1,860,623 + metN BNILDI_09275 0.14 -2.2 1,860,624 - metN BNILDI_09275 0.14 -0.0 1,860,624 - metN BNILDI_09275 0.14 +2.9 1,860,725 + metN BNILDI_09275 0.24 +0.9 1,860,792 - metN BNILDI_09275 0.30 +0.1 1,860,838 - metN BNILDI_09275 0.35 +1.0 1,860,838 - metN BNILDI_09275 0.35 -0.2 1,860,977 + metN BNILDI_09275 0.48 -0.0 1,861,029 + metN BNILDI_09275 0.53 -0.1 1,861,029 + metN BNILDI_09275 0.53 +3.5 1,861,030 - metN BNILDI_09275 0.53 +0.1 1,861,030 - metN BNILDI_09275 0.53 -1.1 1,861,030 - metN BNILDI_09275 0.53 -0.1 1,861,359 + metN BNILDI_09275 0.85 +1.6 1,861,360 - metN BNILDI_09275 0.85 +0.1 1,861,368 + metN BNILDI_09275 0.86 -1.1 1,861,368 + metN BNILDI_09275 0.86 +0.1 1,861,368 + metN BNILDI_09275 0.86 +1.2 1,861,368 + metN BNILDI_09275 0.86 +0.5 1,861,368 + metN BNILDI_09275 0.86 -1.3 1,861,369 - metN BNILDI_09275 0.86 +0.1 1,861,369 - metN BNILDI_09275 0.86 -0.2 1,861,371 - metN BNILDI_09275 0.86 +0.1 1,861,377 - metN BNILDI_09275 0.87 +0.3 1,861,521 - -0.3 1,861,532 + +0.3 1,861,533 - -0.4 1,861,533 - -1.0 1,861,533 - +0.4 1,861,537 + +1.3 1,861,538 - +0.1 1,861,538 - +0.7 1,861,625 - -0.4 1,861,665 + +1.5 1,861,680 + +0.5 1,861,693 + -1.8 1,862,066 - gmhB BNILDI_09280 0.64 +1.9 1,862,095 - gmhB BNILDI_09280 0.69 -2.4 1,862,217 - -0.9 1,862,338 - +0.8 1,862,506 + -1.6
Or see this region's nucleotide sequence