Experiment: L-Arabinose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nanQ and nanK overlap by 4 nucleotides nanK and nanE overlap by 4 nucleotides nanE and nanT are separated by 47 nucleotides
BNILDI_01865: nanQ - N-acetylneuraminate anomerase, at 366,361 to 366,825
nanQ
BNILDI_01870: nanK - N-acetylmannosamine kinase, at 366,822 to 367,697
nanK
BNILDI_01875: nanE - Putative N-acetylmannosamine-6-phosphate 2-epimerase, at 367,694 to 368,383
nanE
BNILDI_01880: nanT - sialic acid transporter NanT, at 368,431 to 369,921
nanT
Position (kb) 367
368
369 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 366.694 kb on - strand, within nanQ at 366.694 kb on - strand, within nanQ at 366.784 kb on + strand at 366.808 kb on + strand at 366.809 kb on - strand at 366.809 kb on - strand at 366.809 kb on - strand at 366.862 kb on - strand at 366.931 kb on + strand, within nanK at 366.931 kb on + strand, within nanK at 367.013 kb on + strand, within nanK at 367.041 kb on - strand, within nanK at 367.103 kb on + strand, within nanK at 367.230 kb on - strand, within nanK at 367.287 kb on + strand, within nanK at 367.288 kb on - strand, within nanK at 367.319 kb on + strand, within nanK at 367.357 kb on + strand, within nanK at 367.463 kb on + strand, within nanK at 367.464 kb on - strand, within nanK at 367.464 kb on - strand, within nanK at 367.555 kb on - strand, within nanK at 367.665 kb on - strand at 367.698 kb on - strand at 367.698 kb on - strand at 367.726 kb on - strand at 367.912 kb on + strand, within nanE at 367.927 kb on + strand, within nanE at 368.020 kb on + strand, within nanE at 368.020 kb on + strand, within nanE at 368.122 kb on + strand, within nanE at 368.124 kb on + strand, within nanE at 368.124 kb on + strand, within nanE at 368.124 kb on + strand, within nanE at 368.125 kb on - strand, within nanE at 368.375 kb on + strand at 368.375 kb on + strand at 368.376 kb on - strand at 368.383 kb on + strand at 368.384 kb on - strand at 368.384 kb on - strand at 368.384 kb on - strand at 368.415 kb on - strand at 368.415 kb on - strand at 368.433 kb on - strand at 368.433 kb on - strand at 368.450 kb on - strand at 368.517 kb on + strand at 368.518 kb on - strand at 368.699 kb on + strand, within nanT at 368.699 kb on + strand, within nanT at 368.807 kb on - strand, within nanT at 368.892 kb on - strand, within nanT at 368.999 kb on + strand, within nanT at 368.999 kb on + strand, within nanT at 369.000 kb on - strand, within nanT at 369.000 kb on - strand, within nanT at 369.020 kb on + strand, within nanT at 369.020 kb on + strand, within nanT at 369.114 kb on + strand, within nanT at 369.238 kb on + strand, within nanT at 369.277 kb on - strand, within nanT at 369.277 kb on - strand, within nanT at 369.280 kb on + strand, within nanT at 369.281 kb on - strand, within nanT at 369.348 kb on + strand, within nanT
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arabinose remove 366,694 - nanQ BNILDI_01865 0.72 -1.2 366,694 - nanQ BNILDI_01865 0.72 +0.2 366,784 + +0.5 366,808 + -0.4 366,809 - -0.1 366,809 - -0.7 366,809 - -0.1 366,862 - +1.2 366,931 + nanK BNILDI_01870 0.12 +0.3 366,931 + nanK BNILDI_01870 0.12 +1.7 367,013 + nanK BNILDI_01870 0.22 +0.7 367,041 - nanK BNILDI_01870 0.25 +0.1 367,103 + nanK BNILDI_01870 0.32 +0.1 367,230 - nanK BNILDI_01870 0.47 +1.2 367,287 + nanK BNILDI_01870 0.53 -0.8 367,288 - nanK BNILDI_01870 0.53 -2.0 367,319 + nanK BNILDI_01870 0.57 +1.0 367,357 + nanK BNILDI_01870 0.61 +0.6 367,463 + nanK BNILDI_01870 0.73 -1.2 367,464 - nanK BNILDI_01870 0.73 -0.5 367,464 - nanK BNILDI_01870 0.73 -3.4 367,555 - nanK BNILDI_01870 0.84 -0.5 367,665 - -0.9 367,698 - -0.3 367,698 - -0.5 367,726 - -1.1 367,912 + nanE BNILDI_01875 0.32 -1.0 367,927 + nanE BNILDI_01875 0.34 -0.2 368,020 + nanE BNILDI_01875 0.47 +0.0 368,020 + nanE BNILDI_01875 0.47 -1.3 368,122 + nanE BNILDI_01875 0.62 +1.9 368,124 + nanE BNILDI_01875 0.62 -2.8 368,124 + nanE BNILDI_01875 0.62 -2.7 368,124 + nanE BNILDI_01875 0.62 +0.3 368,125 - nanE BNILDI_01875 0.62 -1.1 368,375 + -4.8 368,375 + +0.4 368,376 - -0.2 368,383 + +1.7 368,384 - +1.0 368,384 - +0.2 368,384 - +0.3 368,415 - +0.2 368,415 - -3.2 368,433 - -0.6 368,433 - -0.3 368,450 - -0.4 368,517 + -0.6 368,518 - -1.4 368,699 + nanT BNILDI_01880 0.18 +1.2 368,699 + nanT BNILDI_01880 0.18 -0.1 368,807 - nanT BNILDI_01880 0.25 -0.2 368,892 - nanT BNILDI_01880 0.31 +0.1 368,999 + nanT BNILDI_01880 0.38 +0.1 368,999 + nanT BNILDI_01880 0.38 +1.4 369,000 - nanT BNILDI_01880 0.38 +0.8 369,000 - nanT BNILDI_01880 0.38 +1.7 369,020 + nanT BNILDI_01880 0.40 -2.0 369,020 + nanT BNILDI_01880 0.40 +0.1 369,114 + nanT BNILDI_01880 0.46 -0.1 369,238 + nanT BNILDI_01880 0.54 -1.5 369,277 - nanT BNILDI_01880 0.57 +1.8 369,277 - nanT BNILDI_01880 0.57 +0.5 369,280 + nanT BNILDI_01880 0.57 -0.0 369,281 - nanT BNILDI_01880 0.57 -0.7 369,348 + nanT BNILDI_01880 0.62 +1.7
Or see this region's nucleotide sequence