Strain Fitness in Escherichia coli ECOR27 around NOLOHH_02320

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdnaQ and rnhA are separated by 55 nucleotidesrnhA and yafS overlap by 4 nucleotidesyafS and gloB are separated by 33 nucleotidesgloB and mltD are separated by 209 nucleotides NOLOHH_02310: dnaQ - DNA polymerase III subunit epsilon, at 372,139 to 372,879 dnaQ NOLOHH_02315: rnhA - ribonuclease HI, at 372,935 to 373,402 rnhA NOLOHH_02320: yafS - Uncharacterized protein YafS, at 373,399 to 374,121 yafS NOLOHH_02325: gloB - hydroxyacylglutathione hydrolase, at 374,155 to 374,910 gloB NOLOHH_02330: mltD - murein transglycosylase D, at 375,120 to 376,340 mltD Position (kb) 373 374 375Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 372.882 kb on - strandat 372.882 kb on - strandat 372.901 kb on - strandat 372.901 kb on - strandat 372.917 kb on - strandat 372.941 kb on - strandat 372.941 kb on - strandat 372.941 kb on - strandat 373.007 kb on - strand, within rnhAat 373.012 kb on - strand, within rnhAat 373.012 kb on - strand, within rnhAat 373.091 kb on - strand, within rnhAat 373.151 kb on + strand, within rnhAat 373.152 kb on - strand, within rnhAat 373.152 kb on - strand, within rnhAat 373.165 kb on + strand, within rnhAat 373.166 kb on - strand, within rnhAat 373.183 kb on + strand, within rnhAat 373.184 kb on - strand, within rnhAat 373.400 kb on + strandat 373.413 kb on + strandat 373.414 kb on - strandat 373.465 kb on - strandat 373.518 kb on + strand, within yafSat 373.518 kb on + strand, within yafSat 373.518 kb on + strand, within yafSat 373.518 kb on + strand, within yafSat 373.519 kb on - strand, within yafSat 373.525 kb on + strand, within yafSat 373.525 kb on + strand, within yafSat 373.525 kb on + strand, within yafSat 373.525 kb on + strand, within yafSat 373.525 kb on + strand, within yafSat 373.526 kb on - strand, within yafSat 373.526 kb on - strand, within yafSat 373.545 kb on + strand, within yafSat 373.558 kb on + strand, within yafSat 373.660 kb on - strand, within yafSat 373.674 kb on + strand, within yafSat 373.674 kb on + strand, within yafSat 373.675 kb on - strand, within yafSat 373.676 kb on + strand, within yafSat 373.676 kb on + strand, within yafSat 373.677 kb on - strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.828 kb on - strand, within yafSat 373.828 kb on - strand, within yafSat 373.828 kb on - strand, within yafSat 373.828 kb on - strand, within yafSat 373.828 kb on - strand, within yafSat 373.828 kb on - strand, within yafSat 373.828 kb on - strand, within yafSat 373.984 kb on - strand, within yafSat 374.142 kb on + strandat 374.142 kb on + strandat 374.143 kb on - strandat 374.149 kb on + strandat 374.150 kb on - strandat 374.150 kb on - strandat 374.150 kb on - strandat 374.154 kb on + strandat 374.154 kb on + strandat 374.155 kb on - strandat 374.169 kb on + strandat 374.170 kb on - strandat 374.170 kb on - strandat 374.170 kb on - strandat 374.191 kb on + strandat 374.191 kb on + strandat 374.191 kb on + strandat 374.191 kb on + strandat 374.191 kb on + strandat 374.191 kb on + strandat 374.191 kb on + strandat 374.191 kb on + strandat 374.192 kb on - strandat 374.192 kb on - strandat 374.261 kb on + strand, within gloBat 374.300 kb on + strand, within gloBat 374.300 kb on + strand, within gloBat 374.300 kb on + strand, within gloBat 374.301 kb on - strand, within gloBat 374.377 kb on + strand, within gloBat 374.377 kb on + strand, within gloBat 374.418 kb on - strand, within gloBat 374.465 kb on + strand, within gloBat 374.465 kb on + strand, within gloBat 374.466 kb on - strand, within gloBat 374.490 kb on - strand, within gloBat 374.496 kb on + strand, within gloBat 374.496 kb on + strand, within gloBat 374.497 kb on - strand, within gloBat 374.504 kb on - strand, within gloBat 374.518 kb on + strand, within gloBat 374.560 kb on - strand, within gloBat 374.605 kb on - strand, within gloBat 374.636 kb on + strand, within gloBat 374.636 kb on + strand, within gloBat 374.637 kb on - strand, within gloBat 374.655 kb on + strand, within gloBat 374.655 kb on + strand, within gloBat 374.656 kb on - strand, within gloBat 374.656 kb on - strand, within gloBat 374.756 kb on + strand, within gloBat 374.757 kb on - strand, within gloBat 374.961 kb on + strandat 374.969 kb on + strandat 374.969 kb on + strandat 374.998 kb on + strandat 375.101 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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372,882 - -2.9
372,882 - +0.1
372,901 - -0.0
372,901 - +0.9
372,917 - -0.5
372,941 - -0.9
372,941 - +1.1
372,941 - +2.4
373,007 - rnhA NOLOHH_02315 0.15 +0.1
373,012 - rnhA NOLOHH_02315 0.16 -0.5
373,012 - rnhA NOLOHH_02315 0.16 +0.7
373,091 - rnhA NOLOHH_02315 0.33 -1.2
373,151 + rnhA NOLOHH_02315 0.46 -2.0
373,152 - rnhA NOLOHH_02315 0.46 +1.0
373,152 - rnhA NOLOHH_02315 0.46 -0.2
373,165 + rnhA NOLOHH_02315 0.49 +0.2
373,166 - rnhA NOLOHH_02315 0.49 -0.7
373,183 + rnhA NOLOHH_02315 0.53 +0.6
373,184 - rnhA NOLOHH_02315 0.53 +1.4
373,400 + +1.0
373,413 + -0.4
373,414 - +0.3
373,465 - +0.3
373,518 + yafS NOLOHH_02320 0.16 +0.6
373,518 + yafS NOLOHH_02320 0.16 +1.2
373,518 + yafS NOLOHH_02320 0.16 +0.2
373,518 + yafS NOLOHH_02320 0.16 +0.8
373,519 - yafS NOLOHH_02320 0.17 -0.3
373,525 + yafS NOLOHH_02320 0.17 -0.3
373,525 + yafS NOLOHH_02320 0.17 +0.3
373,525 + yafS NOLOHH_02320 0.17 +0.3
373,525 + yafS NOLOHH_02320 0.17 +0.9
373,525 + yafS NOLOHH_02320 0.17 -0.6
373,526 - yafS NOLOHH_02320 0.18 +2.3
373,526 - yafS NOLOHH_02320 0.18 +0.3
373,545 + yafS NOLOHH_02320 0.20 +1.1
373,558 + yafS NOLOHH_02320 0.22 +0.7
373,660 - yafS NOLOHH_02320 0.36 -1.3
373,674 + yafS NOLOHH_02320 0.38 +0.3
373,674 + yafS NOLOHH_02320 0.38 +0.0
373,675 - yafS NOLOHH_02320 0.38 +0.5
373,676 + yafS NOLOHH_02320 0.38 -0.3
373,676 + yafS NOLOHH_02320 0.38 +0.1
373,677 - yafS NOLOHH_02320 0.38 -0.3
373,827 + yafS NOLOHH_02320 0.59 -0.1
373,827 + yafS NOLOHH_02320 0.59 +0.3
373,827 + yafS NOLOHH_02320 0.59 -0.0
373,827 + yafS NOLOHH_02320 0.59 -0.3
373,827 + yafS NOLOHH_02320 0.59 +0.0
373,827 + yafS NOLOHH_02320 0.59 +1.2
373,827 + yafS NOLOHH_02320 0.59 +0.8
373,827 + yafS NOLOHH_02320 0.59 +0.4
373,827 + yafS NOLOHH_02320 0.59 -0.2
373,827 + yafS NOLOHH_02320 0.59 +0.3
373,827 + yafS NOLOHH_02320 0.59 +0.3
373,828 - yafS NOLOHH_02320 0.59 -0.3
373,828 - yafS NOLOHH_02320 0.59 -0.1
373,828 - yafS NOLOHH_02320 0.59 +0.6
373,828 - yafS NOLOHH_02320 0.59 -0.5
373,828 - yafS NOLOHH_02320 0.59 +0.6
373,828 - yafS NOLOHH_02320 0.59 -0.4
373,828 - yafS NOLOHH_02320 0.59 -0.9
373,984 - yafS NOLOHH_02320 0.81 +0.2
374,142 + +0.2
374,142 + -0.5
374,143 - -1.0
374,149 + +0.5
374,150 - -0.1
374,150 - -0.2
374,150 - -0.8
374,154 + -0.1
374,154 + -0.2
374,155 - +0.2
374,169 + +0.3
374,170 - +1.2
374,170 - -0.2
374,170 - -3.1
374,191 + +0.8
374,191 + -0.3
374,191 + -0.3
374,191 + +0.9
374,191 + +0.5
374,191 + +0.5
374,191 + +0.2
374,191 + +0.8
374,192 - -2.7
374,192 - +0.3
374,261 + gloB NOLOHH_02325 0.14 -0.2
374,300 + gloB NOLOHH_02325 0.19 +0.9
374,300 + gloB NOLOHH_02325 0.19 -0.4
374,300 + gloB NOLOHH_02325 0.19 +0.2
374,301 - gloB NOLOHH_02325 0.19 -0.2
374,377 + gloB NOLOHH_02325 0.29 -1.1
374,377 + gloB NOLOHH_02325 0.29 +0.6
374,418 - gloB NOLOHH_02325 0.35 +0.4
374,465 + gloB NOLOHH_02325 0.41 -0.3
374,465 + gloB NOLOHH_02325 0.41 +0.3
374,466 - gloB NOLOHH_02325 0.41 -1.5
374,490 - gloB NOLOHH_02325 0.44 +0.7
374,496 + gloB NOLOHH_02325 0.45 +0.2
374,496 + gloB NOLOHH_02325 0.45 +0.5
374,497 - gloB NOLOHH_02325 0.45 +1.6
374,504 - gloB NOLOHH_02325 0.46 -0.2
374,518 + gloB NOLOHH_02325 0.48 -1.1
374,560 - gloB NOLOHH_02325 0.54 +1.5
374,605 - gloB NOLOHH_02325 0.60 +1.6
374,636 + gloB NOLOHH_02325 0.64 +0.6
374,636 + gloB NOLOHH_02325 0.64 +0.4
374,637 - gloB NOLOHH_02325 0.64 +0.4
374,655 + gloB NOLOHH_02325 0.66 +0.7
374,655 + gloB NOLOHH_02325 0.66 +0.8
374,656 - gloB NOLOHH_02325 0.66 -0.1
374,656 - gloB NOLOHH_02325 0.66 +0.6
374,756 + gloB NOLOHH_02325 0.79 +0.4
374,757 - gloB NOLOHH_02325 0.80 +0.3
374,961 + +0.8
374,969 + +0.6
374,969 + +0.4
374,998 + +0.3
375,101 + +0.1

Or see this region's nucleotide sequence