Strain Fitness in Escherichia coli ECOR27 around NOLOHH_00420

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntNOLOHH_00415 and NOLOHH_00420 are separated by 34 nucleotidesNOLOHH_00420 and NOLOHH_00425 are separated by 107 nucleotidesNOLOHH_00425 and NOLOHH_00430 are separated by 420 nucleotides NOLOHH_00415: NOLOHH_00415 - RepB family plasmid replication initiator protein, at 26,918 to 27,922 _00415 NOLOHH_00420: NOLOHH_00420 - hypothetical protein, at 27,957 to 28,409 _00420 NOLOHH_00425: NOLOHH_00425 - excisionase, at 28,517 to 28,894 _00425 NOLOHH_00430: NOLOHH_00430 - Putative integral membrane protein, at 29,315 to 29,530 _00430 Position (kb) 27 28 29Strain fitness (log2 ratio) -1 0 1at 27.844 kb on + strandat 27.909 kb on + strandat 27.910 kb on - strandat 27.928 kb on + strandat 27.928 kb on + strandat 27.928 kb on + strandat 27.929 kb on - strandat 27.973 kb on - strandat 28.049 kb on + strand, within NOLOHH_00420at 28.079 kb on - strand, within NOLOHH_00420at 28.118 kb on - strand, within NOLOHH_00420at 28.225 kb on - strand, within NOLOHH_00420at 28.225 kb on - strand, within NOLOHH_00420at 28.225 kb on - strand, within NOLOHH_00420at 28.360 kb on + strand, within NOLOHH_00420at 28.451 kb on - strandat 28.539 kb on + strandat 28.557 kb on + strand, within NOLOHH_00425at 28.584 kb on + strand, within NOLOHH_00425at 28.584 kb on + strand, within NOLOHH_00425at 29.102 kb on - strandat 29.148 kb on + strandat 29.190 kb on + strandat 29.322 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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27,844 + +0.4
27,909 + +0.1
27,910 - +0.1
27,928 + -0.0
27,928 + +0.0
27,928 + -0.8
27,929 - -0.3
27,973 - +0.8
28,049 + NOLOHH_00420 0.20 -0.1
28,079 - NOLOHH_00420 0.27 +0.9
28,118 - NOLOHH_00420 0.36 +0.5
28,225 - NOLOHH_00420 0.59 -0.2
28,225 - NOLOHH_00420 0.59 -1.2
28,225 - NOLOHH_00420 0.59 -0.3
28,360 + NOLOHH_00420 0.89 +0.2
28,451 - +0.2
28,539 + -0.2
28,557 + NOLOHH_00425 0.11 +0.2
28,584 + NOLOHH_00425 0.18 +0.2
28,584 + NOLOHH_00425 0.18 +0.6
29,102 - +0.3
29,148 + +0.7
29,190 + +1.1
29,322 - +0.3

Or see this region's nucleotide sequence