Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ychT and ychE are separated by 30 nucleotides ychE and OKFHMN_28620 are separated by 26 nucleotides OKFHMN_28620 and OKFHMN_28625 overlap by 25 nucleotides OKFHMN_28625 and oppA are separated by 373 nucleotides
OKFHMN_28610: ychT - Protein YchT, at 5,491,174 to 5,491,227
ychT
OKFHMN_28615: ychE - NAAT family transporter YchE, at 5,491,258 to 5,491,905
ychE
OKFHMN_28620: OKFHMN_28620 - hypothetical protein, at 5,491,932 to 5,492,087
_28620
OKFHMN_28625: OKFHMN_28625 - hypothetical protein, at 5,492,063 to 5,492,224
_28625
OKFHMN_28630: oppA - oligopeptide ABC transporter substrate-binding protein OppA, at 5,492,598 to 5,494,274
oppA
Position (kb) 5491
5492
5493 Strain fitness (log2 ratio) -1
0
1
2
3 at 5491.063 kb on + strand at 5491.107 kb on - strand at 5491.215 kb on + strand, within ychT at 5491.216 kb on - strand, within ychT at 5491.226 kb on - strand at 5491.478 kb on + strand, within ychE at 5491.608 kb on + strand, within ychE at 5491.665 kb on + strand, within ychE at 5491.698 kb on + strand, within ychE at 5491.698 kb on + strand, within ychE at 5491.698 kb on + strand, within ychE at 5491.699 kb on - strand, within ychE at 5491.702 kb on + strand, within ychE at 5491.742 kb on + strand, within ychE at 5491.824 kb on + strand, within ychE at 5492.086 kb on - strand at 5492.144 kb on - strand, within OKFHMN_28625 at 5492.593 kb on + strand at 5492.620 kb on - strand at 5493.005 kb on + strand, within oppA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 5,491,063 + +3.2 5,491,107 - +0.7 5,491,215 + ychT OKFHMN_28610 0.76 +1.8 5,491,216 - ychT OKFHMN_28610 0.78 +0.6 5,491,226 - -0.6 5,491,478 + ychE OKFHMN_28615 0.34 +0.0 5,491,608 + ychE OKFHMN_28615 0.54 -0.2 5,491,665 + ychE OKFHMN_28615 0.63 -1.4 5,491,698 + ychE OKFHMN_28615 0.68 +0.4 5,491,698 + ychE OKFHMN_28615 0.68 +0.8 5,491,698 + ychE OKFHMN_28615 0.68 +1.0 5,491,699 - ychE OKFHMN_28615 0.68 +2.7 5,491,702 + ychE OKFHMN_28615 0.69 +0.2 5,491,742 + ychE OKFHMN_28615 0.75 +0.7 5,491,824 + ychE OKFHMN_28615 0.87 +0.8 5,492,086 - +1.0 5,492,144 - OKFHMN_28625 0.50 -0.3 5,492,593 + +0.3 5,492,620 - +1.5 5,493,005 + oppA OKFHMN_28630 0.24 +0.1
Or see this region's nucleotide sequence