Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ynfC and speG are separated by 2 nucleotides speG and ynfB are separated by 34 nucleotides ynfB and ynfA are separated by 134 nucleotides ynfA and OKFHMN_27805 are separated by 205 nucleotides
OKFHMN_27785: ynfC - YnfC family lipoprotein, at 5,353,125 to 5,353,871
ynfC
OKFHMN_27790: speG - spermidine N1-acetyltransferase, at 5,353,874 to 5,354,434
speG
OKFHMN_27795: ynfB - DUF1283 domain-containing protein, at 5,354,469 to 5,354,810
ynfB
OKFHMN_27800: ynfA - YnfA family protein, at 5,354,945 to 5,355,271
ynfA
OKFHMN_27805: OKFHMN_27805 - Starvation-sensing protein, at 5,355,477 to 5,355,569
_27805
Position (kb) 5354
5355 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5353.514 kb on - strand, within ynfC at 5353.739 kb on + strand, within ynfC at 5354.081 kb on - strand, within speG at 5354.380 kb on - strand at 5354.562 kb on - strand, within ynfB at 5354.825 kb on - strand at 5354.835 kb on - strand at 5354.889 kb on - strand at 5354.900 kb on - strand at 5354.902 kb on + strand at 5354.914 kb on + strand at 5354.975 kb on - strand at 5355.091 kb on - strand, within ynfA at 5355.143 kb on + strand, within ynfA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 5,353,514 - ynfC OKFHMN_27785 0.52 +0.4 5,353,739 + ynfC OKFHMN_27785 0.82 -0.5 5,354,081 - speG OKFHMN_27790 0.37 +0.3 5,354,380 - +1.7 5,354,562 - ynfB OKFHMN_27795 0.27 -0.0 5,354,825 - -2.0 5,354,835 - -1.2 5,354,889 - -0.9 5,354,900 - -3.0 5,354,902 + -0.2 5,354,914 + +2.0 5,354,975 - -0.1 5,355,091 - ynfA OKFHMN_27800 0.45 +1.6 5,355,143 + ynfA OKFHMN_27800 0.61 +1.8
Or see this region's nucleotide sequence