Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cmoA and yecN are separated by 40 nucleotides yecN and yecE are separated by 52 nucleotides yecE and yecD overlap by 4 nucleotides yecD and aspS are separated by 309 nucleotides
OKFHMN_26225: cmoA - carboxy-S-adenosyl-L-methionine synthase CmoA, at 5,055,044 to 5,055,787
cmoA
OKFHMN_26230: yecN - Inner membrane protein YecN, at 5,055,828 to 5,056,223
yecN
OKFHMN_26235: yecE - UPF0759 protein YecE, at 5,056,276 to 5,057,094
yecE
OKFHMN_26240: yecD - hydrolase, at 5,057,091 to 5,057,657
yecD
OKFHMN_26245: aspS - aspartate--tRNA ligase, at 5,057,967 to 5,059,739
aspS
Position (kb)
5056
5057
5058 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5055.437 kb on - strand, within cmoA at 5055.456 kb on - strand, within cmoA at 5055.545 kb on - strand, within cmoA at 5055.545 kb on - strand, within cmoA at 5055.647 kb on - strand, within cmoA at 5055.647 kb on - strand, within cmoA at 5055.861 kb on - strand at 5055.863 kb on - strand at 5055.999 kb on - strand, within yecN at 5056.065 kb on - strand, within yecN at 5056.224 kb on - strand at 5056.259 kb on + strand at 5056.265 kb on - strand at 5056.356 kb on + strand at 5056.356 kb on + strand at 5056.360 kb on + strand, within yecE at 5056.383 kb on + strand, within yecE at 5056.407 kb on - strand, within yecE at 5056.494 kb on + strand, within yecE at 5056.535 kb on - strand, within yecE at 5057.087 kb on + strand at 5057.121 kb on - strand at 5057.148 kb on - strand, within yecD at 5057.280 kb on + strand, within yecD at 5057.280 kb on + strand, within yecD at 5057.281 kb on - strand, within yecD at 5057.281 kb on - strand, within yecD at 5057.281 kb on - strand, within yecD at 5057.281 kb on - strand, within yecD at 5057.281 kb on - strand, within yecD at 5057.336 kb on - strand, within yecD at 5057.559 kb on + strand, within yecD at 5057.559 kb on + strand, within yecD at 5057.573 kb on + strand, within yecD at 5057.596 kb on - strand, within yecD at 5057.696 kb on - strand at 5057.762 kb on + strand at 5057.763 kb on - strand at 5057.798 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 5,055,437 - cmoA OKFHMN_26225 0.53 -1.3 5,055,456 - cmoA OKFHMN_26225 0.55 +0.5 5,055,545 - cmoA OKFHMN_26225 0.67 -0.8 5,055,545 - cmoA OKFHMN_26225 0.67 +0.3 5,055,647 - cmoA OKFHMN_26225 0.81 -0.2 5,055,647 - cmoA OKFHMN_26225 0.81 +0.4 5,055,861 - -0.5 5,055,863 - -1.7 5,055,999 - yecN OKFHMN_26230 0.43 +1.7 5,056,065 - yecN OKFHMN_26230 0.60 -0.4 5,056,224 - +1.7 5,056,259 + -0.1 5,056,265 - -2.2 5,056,356 + -3.0 5,056,356 + +2.1 5,056,360 + yecE OKFHMN_26235 0.10 -0.6 5,056,383 + yecE OKFHMN_26235 0.13 +0.9 5,056,407 - yecE OKFHMN_26235 0.16 -2.3 5,056,494 + yecE OKFHMN_26235 0.27 +0.3 5,056,535 - yecE OKFHMN_26235 0.32 +0.9 5,057,087 + +0.3 5,057,121 - +0.2 5,057,148 - yecD OKFHMN_26240 0.10 +0.4 5,057,280 + yecD OKFHMN_26240 0.33 +0.3 5,057,280 + yecD OKFHMN_26240 0.33 -1.2 5,057,281 - yecD OKFHMN_26240 0.34 -0.7 5,057,281 - yecD OKFHMN_26240 0.34 -0.8 5,057,281 - yecD OKFHMN_26240 0.34 +1.0 5,057,281 - yecD OKFHMN_26240 0.34 -0.2 5,057,281 - yecD OKFHMN_26240 0.34 -1.3 5,057,336 - yecD OKFHMN_26240 0.43 +0.6 5,057,559 + yecD OKFHMN_26240 0.83 +1.7 5,057,559 + yecD OKFHMN_26240 0.83 +0.4 5,057,573 + yecD OKFHMN_26240 0.85 +1.0 5,057,596 - yecD OKFHMN_26240 0.89 +1.0 5,057,696 - +0.9 5,057,762 + +0.8 5,057,763 - +0.2 5,057,798 - -3.2
Or see this region's nucleotide sequence