Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ldtA and Asn_trna are separated by 55 nucleotides Asn_trna and nac are separated by 325 nucleotides nac and cbl are separated by 101 nucleotides
OKFHMN_25140: ldtA - L,D-transpeptidase, at 4,878,332 to 4,879,267
ldtA
OKFHMN_25145: Asn_trna - tRNA-Asn(gtt), at 4,879,323 to 4,879,398
_trna
OKFHMN_25150: nac - nitrogen assimilation transcriptional regulator NAC, at 4,879,724 to 4,880,641
nac
OKFHMN_25155: cbl - HTH-type transcriptional regulator Cbl, at 4,880,743 to 4,881,693
cbl
Position (kb)
4879
4880
4881 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4878.881 kb on + strand, within ldtA at 4878.896 kb on + strand, within ldtA at 4879.005 kb on + strand, within ldtA at 4879.010 kb on - strand, within ldtA at 4879.266 kb on - strand at 4879.282 kb on - strand at 4879.609 kb on + strand at 4879.631 kb on + strand at 4879.678 kb on + strand at 4879.682 kb on + strand at 4880.055 kb on + strand, within nac at 4880.055 kb on + strand, within nac at 4880.093 kb on + strand, within nac at 4880.166 kb on - strand, within nac at 4880.527 kb on - strand, within nac at 4880.661 kb on + strand at 4880.705 kb on + strand at 4880.759 kb on + strand at 4880.759 kb on + strand at 4881.012 kb on + strand, within cbl at 4881.061 kb on - strand, within cbl at 4881.286 kb on - strand, within cbl at 4881.403 kb on - strand, within cbl at 4881.404 kb on + strand, within cbl at 4881.404 kb on + strand, within cbl at 4881.405 kb on - strand, within cbl at 4881.439 kb on + strand, within cbl at 4881.544 kb on - strand, within cbl at 4881.586 kb on - strand, within cbl at 4881.589 kb on + strand, within cbl
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,878,881 + ldtA OKFHMN_25140 0.59 +0.7 4,878,896 + ldtA OKFHMN_25140 0.60 +0.1 4,879,005 + ldtA OKFHMN_25140 0.72 -0.2 4,879,010 - ldtA OKFHMN_25140 0.72 -0.1 4,879,266 - +0.3 4,879,282 - +2.0 4,879,609 + +0.8 4,879,631 + -2.4 4,879,678 + +0.1 4,879,682 + +0.4 4,880,055 + nac OKFHMN_25150 0.36 +0.2 4,880,055 + nac OKFHMN_25150 0.36 -0.2 4,880,093 + nac OKFHMN_25150 0.40 +0.6 4,880,166 - nac OKFHMN_25150 0.48 +0.1 4,880,527 - nac OKFHMN_25150 0.87 -0.4 4,880,661 + -0.3 4,880,705 + -1.0 4,880,759 + +0.1 4,880,759 + +0.3 4,881,012 + cbl OKFHMN_25155 0.28 -0.4 4,881,061 - cbl OKFHMN_25155 0.33 -0.0 4,881,286 - cbl OKFHMN_25155 0.57 -0.3 4,881,403 - cbl OKFHMN_25155 0.69 -0.2 4,881,404 + cbl OKFHMN_25155 0.70 +1.5 4,881,404 + cbl OKFHMN_25155 0.70 -0.7 4,881,405 - cbl OKFHMN_25155 0.70 +1.2 4,881,439 + cbl OKFHMN_25155 0.73 -2.0 4,881,544 - cbl OKFHMN_25155 0.84 +0.8 4,881,586 - cbl OKFHMN_25155 0.89 +1.4 4,881,589 + cbl OKFHMN_25155 0.89 +0.6
Or see this region's nucleotide sequence