Strain Fitness in Escherichia coli ECRC100 around OKFHMN_25140

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcobT and ldtA are separated by 61 nucleotidesldtA and Asn_trna are separated by 55 nucleotidesAsn_trna and nac are separated by 325 nucleotides OKFHMN_25135: cobT - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 4,877,191 to 4,878,270 cobT OKFHMN_25140: ldtA - L,D-transpeptidase, at 4,878,332 to 4,879,267 ldtA OKFHMN_25145: Asn_trna - tRNA-Asn(gtt), at 4,879,323 to 4,879,398 _trna OKFHMN_25150: nac - nitrogen assimilation transcriptional regulator NAC, at 4,879,724 to 4,880,641 nac Position (kb) 4878 4879 4880Strain fitness (log2 ratio) -2 -1 0 1 2at 4877.568 kb on + strand, within cobTat 4877.660 kb on + strand, within cobTat 4877.660 kb on + strand, within cobTat 4878.225 kb on + strandat 4878.293 kb on + strandat 4878.385 kb on + strandat 4878.520 kb on + strand, within ldtAat 4878.680 kb on - strand, within ldtAat 4878.881 kb on + strand, within ldtAat 4878.896 kb on + strand, within ldtAat 4879.005 kb on + strand, within ldtAat 4879.010 kb on - strand, within ldtAat 4879.266 kb on - strandat 4879.282 kb on - strandat 4879.609 kb on + strandat 4879.631 kb on + strandat 4879.678 kb on + strandat 4879.682 kb on + strandat 4880.055 kb on + strand, within nacat 4880.055 kb on + strand, within nacat 4880.093 kb on + strand, within nacat 4880.166 kb on - strand, within nac

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,877,568 + cobT OKFHMN_25135 0.35 +0.8
4,877,660 + cobT OKFHMN_25135 0.43 -0.5
4,877,660 + cobT OKFHMN_25135 0.43 +0.1
4,878,225 + -0.1
4,878,293 + +1.5
4,878,385 + -0.5
4,878,520 + ldtA OKFHMN_25140 0.20 +0.0
4,878,680 - ldtA OKFHMN_25140 0.37 -0.4
4,878,881 + ldtA OKFHMN_25140 0.59 +0.7
4,878,896 + ldtA OKFHMN_25140 0.60 +0.1
4,879,005 + ldtA OKFHMN_25140 0.72 -0.2
4,879,010 - ldtA OKFHMN_25140 0.72 -0.1
4,879,266 - +0.3
4,879,282 - +2.0
4,879,609 + +0.8
4,879,631 + -2.4
4,879,678 + +0.1
4,879,682 + +0.4
4,880,055 + nac OKFHMN_25150 0.36 +0.2
4,880,055 + nac OKFHMN_25150 0.36 -0.2
4,880,093 + nac OKFHMN_25150 0.40 +0.6
4,880,166 - nac OKFHMN_25150 0.48 +0.1

Or see this region's nucleotide sequence