Strain Fitness in Escherichia coli ECRC100 around OKFHMN_25135

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcobU and cobS overlap by 4 nucleotidescobS and cobT are separated by 11 nucleotidescobT and ldtA are separated by 61 nucleotides OKFHMN_25125: cobU - bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase, at 4,875,897 to 4,876,439 cobU OKFHMN_25130: cobS - adenosylcobinamide-GDP ribazoletransferase, at 4,876,436 to 4,877,179 cobS OKFHMN_25135: cobT - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 4,877,191 to 4,878,270 cobT OKFHMN_25140: ldtA - L,D-transpeptidase, at 4,878,332 to 4,879,267 ldtA Position (kb) 4877 4878 4879Strain fitness (log2 ratio) -2 -1 0 1at 4876.207 kb on - strand, within cobUat 4876.285 kb on + strand, within cobUat 4876.285 kb on + strand, within cobUat 4876.399 kb on + strandat 4876.399 kb on + strandat 4876.399 kb on + strandat 4876.400 kb on - strandat 4876.538 kb on - strand, within cobSat 4876.636 kb on + strand, within cobSat 4876.761 kb on + strand, within cobSat 4876.907 kb on + strand, within cobSat 4876.907 kb on + strand, within cobSat 4876.920 kb on - strand, within cobSat 4877.175 kb on + strandat 4877.244 kb on + strandat 4877.265 kb on + strandat 4877.266 kb on - strandat 4877.568 kb on + strand, within cobTat 4877.660 kb on + strand, within cobTat 4877.660 kb on + strand, within cobTat 4878.225 kb on + strandat 4878.293 kb on + strandat 4878.385 kb on + strandat 4878.520 kb on + strand, within ldtAat 4878.680 kb on - strand, within ldtAat 4878.881 kb on + strand, within ldtAat 4878.896 kb on + strand, within ldtAat 4879.005 kb on + strand, within ldtAat 4879.010 kb on - strand, within ldtAat 4879.266 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,876,207 - cobU OKFHMN_25125 0.57 +0.3
4,876,285 + cobU OKFHMN_25125 0.71 +0.1
4,876,285 + cobU OKFHMN_25125 0.71 -0.1
4,876,399 + -1.6
4,876,399 + +0.3
4,876,399 + +0.1
4,876,400 - -0.9
4,876,538 - cobS OKFHMN_25130 0.14 +0.5
4,876,636 + cobS OKFHMN_25130 0.27 +0.8
4,876,761 + cobS OKFHMN_25130 0.44 -0.7
4,876,907 + cobS OKFHMN_25130 0.63 -0.3
4,876,907 + cobS OKFHMN_25130 0.63 -0.0
4,876,920 - cobS OKFHMN_25130 0.65 -2.2
4,877,175 + +0.6
4,877,244 + -1.1
4,877,265 + -0.1
4,877,266 - +0.1
4,877,568 + cobT OKFHMN_25135 0.35 +0.8
4,877,660 + cobT OKFHMN_25135 0.43 -0.5
4,877,660 + cobT OKFHMN_25135 0.43 +0.1
4,878,225 + -0.1
4,878,293 + +1.5
4,878,385 + -0.5
4,878,520 + ldtA OKFHMN_25140 0.20 +0.0
4,878,680 - ldtA OKFHMN_25140 0.37 -0.4
4,878,881 + ldtA OKFHMN_25140 0.59 +0.7
4,878,896 + ldtA OKFHMN_25140 0.60 +0.1
4,879,005 + ldtA OKFHMN_25140 0.72 -0.2
4,879,010 - ldtA OKFHMN_25140 0.72 -0.1
4,879,266 - +0.3

Or see this region's nucleotide sequence