Strain Fitness in Escherichia coli ECRC100 around OKFHMN_24250

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyegI and yegD are separated by 12 nucleotidesyegD and alkA are separated by 181 nucleotides OKFHMN_24245: yegI - protein kinase YegI, at 4,728,975 to 4,730,915 yegI OKFHMN_24250: yegD - molecular chaperone, at 4,730,928 to 4,732,232 yegD OKFHMN_24255: alkA - DNA-3-methyladenine glycosylase 2, at 4,732,414 to 4,733,262 alkA Position (kb) 4730 4731 4732 4733Strain fitness (log2 ratio) -2 -1 0 1at 4730.003 kb on + strand, within yegIat 4730.059 kb on + strand, within yegIat 4730.146 kb on - strand, within yegIat 4730.223 kb on - strand, within yegIat 4730.334 kb on + strand, within yegIat 4730.334 kb on + strand, within yegIat 4730.344 kb on + strand, within yegIat 4730.860 kb on + strandat 4731.070 kb on + strand, within yegDat 4731.375 kb on - strand, within yegDat 4731.380 kb on - strand, within yegDat 4731.399 kb on + strand, within yegDat 4731.474 kb on - strand, within yegDat 4731.667 kb on - strand, within yegDat 4731.928 kb on - strand, within yegDat 4732.064 kb on - strand, within yegDat 4732.286 kb on - strandat 4732.286 kb on - strandat 4732.419 kb on + strandat 4732.584 kb on + strand, within alkAat 4732.595 kb on + strand, within alkAat 4732.867 kb on + strand, within alkAat 4732.867 kb on + strand, within alkAat 4732.868 kb on - strand, within alkAat 4733.128 kb on + strand, within alkAat 4733.224 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,730,003 + yegI OKFHMN_24245 0.53 +0.5
4,730,059 + yegI OKFHMN_24245 0.56 -0.3
4,730,146 - yegI OKFHMN_24245 0.60 -0.1
4,730,223 - yegI OKFHMN_24245 0.64 +1.1
4,730,334 + yegI OKFHMN_24245 0.70 -0.1
4,730,334 + yegI OKFHMN_24245 0.70 -0.0
4,730,344 + yegI OKFHMN_24245 0.71 -1.4
4,730,860 + -0.2
4,731,070 + yegD OKFHMN_24250 0.11 -2.4
4,731,375 - yegD OKFHMN_24250 0.34 +0.1
4,731,380 - yegD OKFHMN_24250 0.35 -0.7
4,731,399 + yegD OKFHMN_24250 0.36 -0.2
4,731,474 - yegD OKFHMN_24250 0.42 -0.1
4,731,667 - yegD OKFHMN_24250 0.57 -0.3
4,731,928 - yegD OKFHMN_24250 0.77 -0.2
4,732,064 - yegD OKFHMN_24250 0.87 -0.5
4,732,286 - -1.5
4,732,286 - +0.2
4,732,419 + +0.7
4,732,584 + alkA OKFHMN_24255 0.20 -0.1
4,732,595 + alkA OKFHMN_24255 0.21 -0.5
4,732,867 + alkA OKFHMN_24255 0.53 -1.5
4,732,867 + alkA OKFHMN_24255 0.53 -1.7
4,732,868 - alkA OKFHMN_24255 0.53 -1.0
4,733,128 + alkA OKFHMN_24255 0.84 -0.2
4,733,224 - +1.1

Or see this region's nucleotide sequence