Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uhpC and gtdA are separated by 11 nucleotides gtdA and ycgM are separated by 14 nucleotides ycgM and maiA are separated by 8 nucleotides
OKFHMN_23590: uhpC - aromatic acid/H+ symport family MFS transporter, at 4,608,905 to 4,610,263
uhpC
OKFHMN_23595: gtdA - gentisate 1,2-dioxygenase, at 4,610,275 to 4,611,303
gtdA
OKFHMN_23600: ycgM - 5-carboxymethyl-2-hydroxymuconate isomerase, at 4,611,318 to 4,612,019
ycgM
OKFHMN_23605: maiA - maleylacetoacetate isomerase, at 4,612,028 to 4,612,672
maiA
Position (kb) 4610
4611
4612 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 4609.316 kb on + strand, within uhpC at 4609.317 kb on - strand, within uhpC at 4609.317 kb on - strand, within uhpC at 4609.369 kb on + strand, within uhpC at 4609.488 kb on + strand, within uhpC at 4609.711 kb on + strand, within uhpC at 4609.716 kb on - strand, within uhpC at 4609.815 kb on + strand, within uhpC at 4609.880 kb on + strand, within uhpC at 4609.881 kb on - strand, within uhpC at 4609.881 kb on - strand, within uhpC at 4609.887 kb on + strand, within uhpC at 4609.894 kb on + strand, within uhpC at 4609.894 kb on + strand, within uhpC at 4609.895 kb on - strand, within uhpC at 4609.896 kb on + strand, within uhpC at 4609.896 kb on + strand, within uhpC at 4609.897 kb on - strand, within uhpC at 4610.320 kb on - strand at 4610.388 kb on + strand, within gtdA at 4610.537 kb on + strand, within gtdA at 4610.614 kb on - strand, within gtdA at 4610.638 kb on - strand, within gtdA at 4610.845 kb on + strand, within gtdA at 4610.845 kb on + strand, within gtdA at 4610.845 kb on + strand, within gtdA at 4610.868 kb on + strand, within gtdA at 4610.913 kb on + strand, within gtdA at 4610.918 kb on - strand, within gtdA at 4610.918 kb on - strand, within gtdA at 4611.112 kb on + strand, within gtdA at 4611.112 kb on + strand, within gtdA at 4611.112 kb on + strand, within gtdA at 4611.112 kb on + strand, within gtdA at 4611.112 kb on + strand, within gtdA at 4611.112 kb on + strand, within gtdA at 4611.113 kb on - strand, within gtdA at 4611.113 kb on - strand, within gtdA at 4611.264 kb on - strand at 4611.331 kb on + strand at 4611.420 kb on + strand, within ycgM at 4611.554 kb on + strand, within ycgM at 4611.558 kb on + strand, within ycgM at 4611.633 kb on + strand, within ycgM at 4611.633 kb on + strand, within ycgM at 4611.634 kb on - strand, within ycgM at 4611.805 kb on + strand, within ycgM at 4611.805 kb on + strand, within ycgM at 4611.879 kb on + strand, within ycgM at 4612.052 kb on - strand at 4612.103 kb on + strand, within maiA at 4612.123 kb on + strand, within maiA at 4612.124 kb on - strand, within maiA at 4612.257 kb on - strand, within maiA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,609,316 + uhpC OKFHMN_23590 0.30 -0.8 4,609,317 - uhpC OKFHMN_23590 0.30 -0.0 4,609,317 - uhpC OKFHMN_23590 0.30 +0.3 4,609,369 + uhpC OKFHMN_23590 0.34 +0.6 4,609,488 + uhpC OKFHMN_23590 0.43 -0.1 4,609,711 + uhpC OKFHMN_23590 0.59 -1.0 4,609,716 - uhpC OKFHMN_23590 0.60 -1.5 4,609,815 + uhpC OKFHMN_23590 0.67 +0.2 4,609,880 + uhpC OKFHMN_23590 0.72 -1.1 4,609,881 - uhpC OKFHMN_23590 0.72 +1.0 4,609,881 - uhpC OKFHMN_23590 0.72 +1.6 4,609,887 + uhpC OKFHMN_23590 0.72 +0.6 4,609,894 + uhpC OKFHMN_23590 0.73 +1.7 4,609,894 + uhpC OKFHMN_23590 0.73 +0.9 4,609,895 - uhpC OKFHMN_23590 0.73 +3.2 4,609,896 + uhpC OKFHMN_23590 0.73 +0.8 4,609,896 + uhpC OKFHMN_23590 0.73 +0.1 4,609,897 - uhpC OKFHMN_23590 0.73 -1.3 4,610,320 - -0.0 4,610,388 + gtdA OKFHMN_23595 0.11 -0.0 4,610,537 + gtdA OKFHMN_23595 0.25 +0.3 4,610,614 - gtdA OKFHMN_23595 0.33 +0.1 4,610,638 - gtdA OKFHMN_23595 0.35 -0.4 4,610,845 + gtdA OKFHMN_23595 0.55 +0.8 4,610,845 + gtdA OKFHMN_23595 0.55 -0.1 4,610,845 + gtdA OKFHMN_23595 0.55 -1.0 4,610,868 + gtdA OKFHMN_23595 0.58 +0.6 4,610,913 + gtdA OKFHMN_23595 0.62 -0.7 4,610,918 - gtdA OKFHMN_23595 0.62 -0.1 4,610,918 - gtdA OKFHMN_23595 0.62 +0.2 4,611,112 + gtdA OKFHMN_23595 0.81 -0.4 4,611,112 + gtdA OKFHMN_23595 0.81 -0.0 4,611,112 + gtdA OKFHMN_23595 0.81 -4.9 4,611,112 + gtdA OKFHMN_23595 0.81 +0.3 4,611,112 + gtdA OKFHMN_23595 0.81 +0.3 4,611,112 + gtdA OKFHMN_23595 0.81 +1.1 4,611,113 - gtdA OKFHMN_23595 0.81 -0.8 4,611,113 - gtdA OKFHMN_23595 0.81 -0.7 4,611,264 - +0.2 4,611,331 + +0.6 4,611,420 + ycgM OKFHMN_23600 0.15 -0.5 4,611,554 + ycgM OKFHMN_23600 0.34 -3.2 4,611,558 + ycgM OKFHMN_23600 0.34 +0.1 4,611,633 + ycgM OKFHMN_23600 0.45 +0.5 4,611,633 + ycgM OKFHMN_23600 0.45 -1.6 4,611,634 - ycgM OKFHMN_23600 0.45 -0.5 4,611,805 + ycgM OKFHMN_23600 0.69 +0.9 4,611,805 + ycgM OKFHMN_23600 0.69 +0.9 4,611,879 + ycgM OKFHMN_23600 0.80 +0.6 4,612,052 - -1.9 4,612,103 + maiA OKFHMN_23605 0.12 -0.4 4,612,123 + maiA OKFHMN_23605 0.15 +0.3 4,612,124 - maiA OKFHMN_23605 0.15 +0.1 4,612,257 - maiA OKFHMN_23605 0.36 -1.5
Or see this region's nucleotide sequence