Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yojI and mqo are separated by 217 nucleotides mqo and eco are separated by 149 nucleotides eco and OKFHMN_23235 are separated by 133 nucleotides OKFHMN_23235 and OKFHMN_23240 are separated by 51 nucleotides
OKFHMN_23220: yojI - microcin J25 efflux ABC transporter YojI, at 4,531,755 to 4,533,398
yojI
OKFHMN_23225: mqo - malate dehydrogenase (quinone), at 4,533,616 to 4,535,262
mqo
OKFHMN_23230: eco - serine protease inhibitor ecotin, at 4,535,412 to 4,535,900
eco
OKFHMN_23235: OKFHMN_23235 - hypothetical protein, at 4,536,034 to 4,536,198
_23235
OKFHMN_23240: OKFHMN_23240 - Orf 5, at 4,536,250 to 4,536,291
_23240
Position (kb)
4533
4534
4535
4536 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4532.937 kb on + strand, within yojI at 4532.963 kb on + strand, within yojI at 4533.073 kb on + strand, within yojI at 4533.086 kb on + strand, within yojI at 4533.288 kb on + strand at 4533.288 kb on + strand at 4533.441 kb on - strand at 4533.441 kb on - strand at 4533.467 kb on - strand at 4533.601 kb on + strand at 4533.716 kb on + strand at 4533.716 kb on + strand at 4533.761 kb on - strand at 4533.764 kb on + strand at 4533.886 kb on + strand, within mqo at 4533.886 kb on + strand, within mqo at 4534.126 kb on + strand, within mqo at 4534.166 kb on + strand, within mqo at 4534.237 kb on + strand, within mqo at 4534.442 kb on + strand, within mqo at 4534.531 kb on - strand, within mqo at 4534.916 kb on + strand, within mqo at 4535.071 kb on - strand, within mqo at 4535.095 kb on + strand, within mqo at 4535.196 kb on + strand at 4535.196 kb on + strand at 4535.196 kb on + strand at 4535.260 kb on + strand at 4535.260 kb on + strand at 4535.261 kb on - strand at 4535.286 kb on - strand at 4535.286 kb on - strand at 4535.302 kb on - strand at 4535.302 kb on - strand at 4535.351 kb on - strand at 4535.473 kb on - strand, within eco at 4535.489 kb on - strand, within eco at 4535.489 kb on - strand, within eco at 4535.516 kb on - strand, within eco at 4535.542 kb on + strand, within eco at 4535.543 kb on - strand, within eco at 4535.610 kb on - strand, within eco at 4535.877 kb on + strand at 4535.960 kb on - strand at 4536.179 kb on - strand, within OKFHMN_23235 at 4536.214 kb on + strand at 4536.245 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,532,937 + yojI OKFHMN_23220 0.72 +0.3 4,532,963 + yojI OKFHMN_23220 0.73 +0.0 4,533,073 + yojI OKFHMN_23220 0.80 +0.1 4,533,086 + yojI OKFHMN_23220 0.81 -1.7 4,533,288 + -1.9 4,533,288 + -1.8 4,533,441 - +0.9 4,533,441 - +0.4 4,533,467 - +0.3 4,533,601 + -1.0 4,533,716 + -0.2 4,533,716 + -0.1 4,533,761 - -0.1 4,533,764 + +0.1 4,533,886 + mqo OKFHMN_23225 0.16 +0.3 4,533,886 + mqo OKFHMN_23225 0.16 -1.0 4,534,126 + mqo OKFHMN_23225 0.31 +1.5 4,534,166 + mqo OKFHMN_23225 0.33 -0.3 4,534,237 + mqo OKFHMN_23225 0.38 -0.1 4,534,442 + mqo OKFHMN_23225 0.50 +0.9 4,534,531 - mqo OKFHMN_23225 0.56 +0.0 4,534,916 + mqo OKFHMN_23225 0.79 -0.6 4,535,071 - mqo OKFHMN_23225 0.88 -0.7 4,535,095 + mqo OKFHMN_23225 0.90 -0.8 4,535,196 + +0.0 4,535,196 + +0.5 4,535,196 + +0.5 4,535,260 + +0.1 4,535,260 + -0.1 4,535,261 - +0.0 4,535,286 - +0.4 4,535,286 - +0.1 4,535,302 - -0.2 4,535,302 - +0.1 4,535,351 - -1.2 4,535,473 - eco OKFHMN_23230 0.12 -0.5 4,535,489 - eco OKFHMN_23230 0.16 -3.7 4,535,489 - eco OKFHMN_23230 0.16 -0.4 4,535,516 - eco OKFHMN_23230 0.21 +1.4 4,535,542 + eco OKFHMN_23230 0.27 +0.7 4,535,543 - eco OKFHMN_23230 0.27 -0.1 4,535,610 - eco OKFHMN_23230 0.40 +0.9 4,535,877 + +0.4 4,535,960 - +2.4 4,536,179 - OKFHMN_23235 0.88 +0.4 4,536,214 + +0.6 4,536,245 + +0.9
Or see this region's nucleotide sequence