Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisQ and hisM overlap by 4 nucleotides hisM and hisP are separated by 7 nucleotides hisP and rpnB are separated by 196 nucleotides
OKFHMN_22735: hisQ - histidine ABC transporter permease HisQ, at 4,422,583 to 4,423,269
hisQ
OKFHMN_22740: hisM - Histidine transport system permease protein HisM, at 4,423,266 to 4,423,982
hisM
OKFHMN_22745: hisP - histidine ABC transporter ATP-binding protein HisP, at 4,423,990 to 4,424,763
hisP
OKFHMN_22750: rpnB - recombination-promoting nuclease RpnB, at 4,424,960 to 4,425,850
rpnB
Position (kb)
4423
4424
4425 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4423.075 kb on - strand, within hisQ at 4423.425 kb on + strand, within hisM at 4423.512 kb on + strand, within hisM at 4423.513 kb on - strand, within hisM at 4423.513 kb on - strand, within hisM at 4423.529 kb on + strand, within hisM at 4423.737 kb on - strand, within hisM at 4423.784 kb on + strand, within hisM at 4423.855 kb on - strand, within hisM at 4423.908 kb on + strand, within hisM at 4423.909 kb on - strand, within hisM at 4423.909 kb on - strand, within hisM at 4424.088 kb on - strand, within hisP at 4424.088 kb on - strand, within hisP at 4424.090 kb on + strand, within hisP at 4424.124 kb on + strand, within hisP at 4424.142 kb on + strand, within hisP at 4424.308 kb on - strand, within hisP at 4424.646 kb on + strand, within hisP at 4424.678 kb on + strand, within hisP at 4424.746 kb on - strand at 4424.761 kb on + strand at 4424.762 kb on - strand at 4425.405 kb on + strand, within rpnB at 4425.460 kb on + strand, within rpnB at 4425.531 kb on + strand, within rpnB at 4425.559 kb on + strand, within rpnB at 4425.585 kb on + strand, within rpnB at 4425.598 kb on + strand, within rpnB at 4425.598 kb on + strand, within rpnB at 4425.598 kb on + strand, within rpnB at 4425.603 kb on - strand, within rpnB at 4425.607 kb on - strand, within rpnB at 4425.607 kb on - strand, within rpnB at 4425.610 kb on - strand, within rpnB at 4425.655 kb on - strand, within rpnB at 4425.709 kb on - strand, within rpnB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,423,075 - hisQ OKFHMN_22735 0.72 +0.6 4,423,425 + hisM OKFHMN_22740 0.22 -0.2 4,423,512 + hisM OKFHMN_22740 0.34 +0.3 4,423,513 - hisM OKFHMN_22740 0.34 -0.0 4,423,513 - hisM OKFHMN_22740 0.34 +0.0 4,423,529 + hisM OKFHMN_22740 0.37 +1.8 4,423,737 - hisM OKFHMN_22740 0.66 -1.5 4,423,784 + hisM OKFHMN_22740 0.72 +1.1 4,423,855 - hisM OKFHMN_22740 0.82 -0.7 4,423,908 + hisM OKFHMN_22740 0.90 +0.7 4,423,909 - hisM OKFHMN_22740 0.90 -0.1 4,423,909 - hisM OKFHMN_22740 0.90 -0.3 4,424,088 - hisP OKFHMN_22745 0.13 +1.6 4,424,088 - hisP OKFHMN_22745 0.13 +0.6 4,424,090 + hisP OKFHMN_22745 0.13 -0.6 4,424,124 + hisP OKFHMN_22745 0.17 +0.5 4,424,142 + hisP OKFHMN_22745 0.20 +0.7 4,424,308 - hisP OKFHMN_22745 0.41 +0.5 4,424,646 + hisP OKFHMN_22745 0.85 -0.1 4,424,678 + hisP OKFHMN_22745 0.89 +0.2 4,424,746 - +2.0 4,424,761 + +0.4 4,424,762 - +2.4 4,425,405 + rpnB OKFHMN_22750 0.50 -0.2 4,425,460 + rpnB OKFHMN_22750 0.56 +0.2 4,425,531 + rpnB OKFHMN_22750 0.64 +0.0 4,425,559 + rpnB OKFHMN_22750 0.67 +0.5 4,425,585 + rpnB OKFHMN_22750 0.70 -0.4 4,425,598 + rpnB OKFHMN_22750 0.72 -1.8 4,425,598 + rpnB OKFHMN_22750 0.72 -0.8 4,425,598 + rpnB OKFHMN_22750 0.72 -0.1 4,425,603 - rpnB OKFHMN_22750 0.72 +0.5 4,425,607 - rpnB OKFHMN_22750 0.73 +0.2 4,425,607 - rpnB OKFHMN_22750 0.73 -0.3 4,425,610 - rpnB OKFHMN_22750 0.73 +0.5 4,425,655 - rpnB OKFHMN_22750 0.78 +0.4 4,425,709 - rpnB OKFHMN_22750 0.84 -0.3
Or see this region's nucleotide sequence