Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt emrY and dsdA are separated by 107 nucleotides dsdA and dsdX are separated by 17 nucleotides dsdX and purR are separated by 284 nucleotides
OKFHMN_22445: emrY - multidrug efflux MFS transporter permease subunit EmrY, at 4,364,266 to 4,365,804
emrY
OKFHMN_22450: dsdA - D-serine ammonia-lyase, at 4,365,912 to 4,367,240
dsdA
OKFHMN_22455: dsdX - D-serine transporter DsdX, at 4,367,258 to 4,367,476
dsdX
OKFHMN_22460: purR - transcriptional regulator, at 4,367,761 to 4,368,714
purR
Position (kb)
4365
4366
4367
4368 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4365.096 kb on + strand, within emrY at 4365.180 kb on + strand, within emrY at 4365.249 kb on - strand, within emrY at 4365.270 kb on - strand, within emrY at 4365.307 kb on + strand, within emrY at 4365.422 kb on + strand, within emrY at 4365.565 kb on - strand, within emrY at 4365.746 kb on + strand at 4365.823 kb on - strand at 4365.824 kb on + strand at 4365.910 kb on + strand at 4366.038 kb on + strand at 4366.135 kb on + strand, within dsdA at 4366.135 kb on + strand, within dsdA at 4366.136 kb on - strand, within dsdA at 4366.158 kb on - strand, within dsdA at 4366.307 kb on + strand, within dsdA at 4366.307 kb on + strand, within dsdA at 4366.307 kb on + strand, within dsdA at 4366.368 kb on - strand, within dsdA at 4366.449 kb on - strand, within dsdA at 4366.499 kb on - strand, within dsdA at 4366.595 kb on - strand, within dsdA at 4366.595 kb on - strand, within dsdA at 4366.673 kb on + strand, within dsdA at 4366.673 kb on + strand, within dsdA at 4366.673 kb on + strand, within dsdA at 4366.674 kb on - strand, within dsdA at 4366.674 kb on - strand, within dsdA at 4366.674 kb on - strand, within dsdA at 4366.674 kb on - strand, within dsdA at 4366.674 kb on - strand, within dsdA at 4366.760 kb on - strand, within dsdA at 4366.779 kb on - strand, within dsdA at 4366.805 kb on - strand, within dsdA at 4366.890 kb on + strand, within dsdA at 4367.013 kb on - strand, within dsdA at 4367.057 kb on - strand, within dsdA at 4367.119 kb on + strand at 4367.120 kb on - strand at 4367.120 kb on - strand at 4367.120 kb on - strand at 4367.125 kb on - strand at 4367.125 kb on - strand at 4367.125 kb on - strand at 4367.157 kb on - strand at 4367.243 kb on + strand at 4367.244 kb on - strand at 4367.260 kb on - strand at 4367.260 kb on - strand at 4367.311 kb on + strand, within dsdX at 4367.329 kb on - strand, within dsdX at 4367.486 kb on + strand at 4367.487 kb on - strand at 4367.544 kb on + strand at 4367.545 kb on - strand at 4367.547 kb on - strand at 4367.602 kb on - strand at 4367.619 kb on + strand at 4367.619 kb on + strand at 4367.619 kb on + strand at 4367.620 kb on - strand at 4367.825 kb on + strand at 4367.852 kb on - strand at 4367.896 kb on - strand, within purR at 4367.896 kb on - strand, within purR at 4367.955 kb on + strand, within purR at 4368.085 kb on + strand, within purR at 4368.085 kb on + strand, within purR at 4368.085 kb on + strand, within purR at 4368.086 kb on - strand, within purR at 4368.091 kb on - strand, within purR at 4368.130 kb on + strand, within purR at 4368.187 kb on + strand, within purR
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,365,096 + emrY OKFHMN_22445 0.54 +1.3 4,365,180 + emrY OKFHMN_22445 0.59 +0.8 4,365,249 - emrY OKFHMN_22445 0.64 +0.1 4,365,270 - emrY OKFHMN_22445 0.65 +0.8 4,365,307 + emrY OKFHMN_22445 0.68 -0.5 4,365,422 + emrY OKFHMN_22445 0.75 -0.8 4,365,565 - emrY OKFHMN_22445 0.84 -1.7 4,365,746 + -0.7 4,365,823 - +0.9 4,365,824 + +0.9 4,365,910 + +0.7 4,366,038 + -2.7 4,366,135 + dsdA OKFHMN_22450 0.17 -0.5 4,366,135 + dsdA OKFHMN_22450 0.17 -0.4 4,366,136 - dsdA OKFHMN_22450 0.17 -0.1 4,366,158 - dsdA OKFHMN_22450 0.19 -2.6 4,366,307 + dsdA OKFHMN_22450 0.30 -1.2 4,366,307 + dsdA OKFHMN_22450 0.30 +0.0 4,366,307 + dsdA OKFHMN_22450 0.30 +1.0 4,366,368 - dsdA OKFHMN_22450 0.34 -1.2 4,366,449 - dsdA OKFHMN_22450 0.40 -0.7 4,366,499 - dsdA OKFHMN_22450 0.44 +0.1 4,366,595 - dsdA OKFHMN_22450 0.51 -1.2 4,366,595 - dsdA OKFHMN_22450 0.51 -0.9 4,366,673 + dsdA OKFHMN_22450 0.57 -2.2 4,366,673 + dsdA OKFHMN_22450 0.57 -0.1 4,366,673 + dsdA OKFHMN_22450 0.57 -1.0 4,366,674 - dsdA OKFHMN_22450 0.57 -0.8 4,366,674 - dsdA OKFHMN_22450 0.57 -0.6 4,366,674 - dsdA OKFHMN_22450 0.57 +0.2 4,366,674 - dsdA OKFHMN_22450 0.57 +0.0 4,366,674 - dsdA OKFHMN_22450 0.57 +0.2 4,366,760 - dsdA OKFHMN_22450 0.64 -0.4 4,366,779 - dsdA OKFHMN_22450 0.65 +0.2 4,366,805 - dsdA OKFHMN_22450 0.67 -0.8 4,366,890 + dsdA OKFHMN_22450 0.74 -0.8 4,367,013 - dsdA OKFHMN_22450 0.83 -0.8 4,367,057 - dsdA OKFHMN_22450 0.86 +0.1 4,367,119 + +2.0 4,367,120 - -1.0 4,367,120 - -0.7 4,367,120 - -1.7 4,367,125 - -3.3 4,367,125 - +0.7 4,367,125 - -0.0 4,367,157 - -0.4 4,367,243 + -0.5 4,367,244 - -0.1 4,367,260 - +0.2 4,367,260 - +0.7 4,367,311 + dsdX OKFHMN_22455 0.24 +0.5 4,367,329 - dsdX OKFHMN_22455 0.32 -0.3 4,367,486 + -0.2 4,367,487 - -0.1 4,367,544 + -0.0 4,367,545 - -0.3 4,367,547 - +0.8 4,367,602 - -0.7 4,367,619 + +0.1 4,367,619 + -0.0 4,367,619 + -1.3 4,367,620 - -0.4 4,367,825 + +0.3 4,367,852 - -0.5 4,367,896 - purR OKFHMN_22460 0.14 -2.3 4,367,896 - purR OKFHMN_22460 0.14 +1.3 4,367,955 + purR OKFHMN_22460 0.20 -0.4 4,368,085 + purR OKFHMN_22460 0.34 -0.4 4,368,085 + purR OKFHMN_22460 0.34 -1.4 4,368,085 + purR OKFHMN_22460 0.34 +1.1 4,368,086 - purR OKFHMN_22460 0.34 -1.4 4,368,091 - purR OKFHMN_22460 0.35 +1.0 4,368,130 + purR OKFHMN_22460 0.39 -1.1 4,368,187 + purR OKFHMN_22460 0.45 -1.5
Or see this region's nucleotide sequence