Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysA and cysM are separated by 133 nucleotides cysM and yfeK are separated by 37 nucleotides yfeK and pdxK are separated by 104 nucleotides
OKFHMN_22165: cysA - sulfate/thiosulfate ABC transporter ATP-binding protein CysA, at 4,310,334 to 4,311,431
cysA
OKFHMN_22170: cysM - cysteine synthase B, at 4,311,565 to 4,312,476
cysM
OKFHMN_22175: yfeK - Uncharacterized protein YfeK, at 4,312,514 to 4,312,846
yfeK
OKFHMN_22180: pdxK - pyridoxine/pyridoxal/pyridoxamine kinase, at 4,312,951 to 4,313,802
pdxK
Position (kb)
4311
4312
4313 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4310.791 kb on + strand, within cysA at 4310.976 kb on - strand, within cysA at 4311.004 kb on + strand, within cysA at 4311.120 kb on + strand, within cysA at 4311.221 kb on - strand, within cysA at 4311.246 kb on + strand, within cysA at 4311.279 kb on + strand, within cysA at 4311.369 kb on + strand at 4311.369 kb on + strand at 4311.508 kb on - strand at 4311.508 kb on - strand at 4311.552 kb on - strand at 4311.575 kb on + strand at 4311.880 kb on + strand, within cysM at 4312.023 kb on + strand, within cysM at 4312.063 kb on - strand, within cysM at 4312.098 kb on + strand, within cysM at 4312.314 kb on - strand, within cysM at 4312.475 kb on - strand at 4312.475 kb on - strand at 4312.524 kb on + strand at 4312.524 kb on + strand at 4312.572 kb on + strand, within yfeK at 4312.573 kb on - strand, within yfeK at 4312.609 kb on + strand, within yfeK at 4312.694 kb on - strand, within yfeK at 4312.694 kb on - strand, within yfeK at 4312.852 kb on + strand at 4312.858 kb on - strand at 4312.870 kb on + strand at 4312.956 kb on + strand at 4312.971 kb on + strand at 4313.029 kb on + strand at 4313.118 kb on + strand, within pdxK at 4313.226 kb on - strand, within pdxK at 4313.236 kb on + strand, within pdxK at 4313.237 kb on - strand, within pdxK at 4313.238 kb on + strand, within pdxK at 4313.309 kb on + strand, within pdxK
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,310,791 + cysA OKFHMN_22165 0.42 -0.2 4,310,976 - cysA OKFHMN_22165 0.58 +0.2 4,311,004 + cysA OKFHMN_22165 0.61 +0.0 4,311,120 + cysA OKFHMN_22165 0.72 -0.0 4,311,221 - cysA OKFHMN_22165 0.81 -2.2 4,311,246 + cysA OKFHMN_22165 0.83 +0.5 4,311,279 + cysA OKFHMN_22165 0.86 +1.0 4,311,369 + +0.7 4,311,369 + +0.3 4,311,508 - +0.4 4,311,508 - -2.4 4,311,552 - -0.0 4,311,575 + -0.7 4,311,880 + cysM OKFHMN_22170 0.35 +0.7 4,312,023 + cysM OKFHMN_22170 0.50 -0.1 4,312,063 - cysM OKFHMN_22170 0.55 +1.3 4,312,098 + cysM OKFHMN_22170 0.58 +2.0 4,312,314 - cysM OKFHMN_22170 0.82 -0.3 4,312,475 - -0.0 4,312,475 - -0.1 4,312,524 + +1.0 4,312,524 + -0.1 4,312,572 + yfeK OKFHMN_22175 0.17 +0.3 4,312,573 - yfeK OKFHMN_22175 0.18 -0.6 4,312,609 + yfeK OKFHMN_22175 0.29 +1.7 4,312,694 - yfeK OKFHMN_22175 0.54 +0.1 4,312,694 - yfeK OKFHMN_22175 0.54 -0.4 4,312,852 + +0.7 4,312,858 - +0.2 4,312,870 + +0.3 4,312,956 + +1.5 4,312,971 + -1.1 4,313,029 + +1.1 4,313,118 + pdxK OKFHMN_22180 0.20 +1.4 4,313,226 - pdxK OKFHMN_22180 0.32 -2.1 4,313,236 + pdxK OKFHMN_22180 0.33 -1.4 4,313,237 - pdxK OKFHMN_22180 0.34 +0.4 4,313,238 + pdxK OKFHMN_22180 0.34 -0.1 4,313,309 + pdxK OKFHMN_22180 0.42 +0.1
Or see this region's nucleotide sequence