Strain Fitness in Escherichia coli ECRC100 around OKFHMN_22170

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcysA and cysM are separated by 133 nucleotidescysM and yfeK are separated by 37 nucleotidesyfeK and pdxK are separated by 104 nucleotides OKFHMN_22165: cysA - sulfate/thiosulfate ABC transporter ATP-binding protein CysA, at 4,310,334 to 4,311,431 cysA OKFHMN_22170: cysM - cysteine synthase B, at 4,311,565 to 4,312,476 cysM OKFHMN_22175: yfeK - Uncharacterized protein YfeK, at 4,312,514 to 4,312,846 yfeK OKFHMN_22180: pdxK - pyridoxine/pyridoxal/pyridoxamine kinase, at 4,312,951 to 4,313,802 pdxK Position (kb) 4311 4312 4313Strain fitness (log2 ratio) -2 -1 0 1 2at 4310.791 kb on + strand, within cysAat 4310.976 kb on - strand, within cysAat 4311.004 kb on + strand, within cysAat 4311.120 kb on + strand, within cysAat 4311.221 kb on - strand, within cysAat 4311.246 kb on + strand, within cysAat 4311.279 kb on + strand, within cysAat 4311.369 kb on + strandat 4311.369 kb on + strandat 4311.508 kb on - strandat 4311.508 kb on - strandat 4311.552 kb on - strandat 4311.575 kb on + strandat 4311.880 kb on + strand, within cysMat 4312.023 kb on + strand, within cysMat 4312.063 kb on - strand, within cysMat 4312.098 kb on + strand, within cysMat 4312.314 kb on - strand, within cysMat 4312.475 kb on - strandat 4312.475 kb on - strandat 4312.524 kb on + strandat 4312.524 kb on + strandat 4312.572 kb on + strand, within yfeKat 4312.573 kb on - strand, within yfeKat 4312.609 kb on + strand, within yfeKat 4312.694 kb on - strand, within yfeKat 4312.694 kb on - strand, within yfeKat 4312.852 kb on + strandat 4312.858 kb on - strandat 4312.870 kb on + strandat 4312.956 kb on + strandat 4312.971 kb on + strandat 4313.029 kb on + strandat 4313.118 kb on + strand, within pdxKat 4313.226 kb on - strand, within pdxKat 4313.236 kb on + strand, within pdxKat 4313.237 kb on - strand, within pdxKat 4313.238 kb on + strand, within pdxKat 4313.309 kb on + strand, within pdxK

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,310,791 + cysA OKFHMN_22165 0.42 -0.2
4,310,976 - cysA OKFHMN_22165 0.58 +0.2
4,311,004 + cysA OKFHMN_22165 0.61 +0.0
4,311,120 + cysA OKFHMN_22165 0.72 -0.0
4,311,221 - cysA OKFHMN_22165 0.81 -2.2
4,311,246 + cysA OKFHMN_22165 0.83 +0.5
4,311,279 + cysA OKFHMN_22165 0.86 +1.0
4,311,369 + +0.7
4,311,369 + +0.3
4,311,508 - +0.4
4,311,508 - -2.4
4,311,552 - -0.0
4,311,575 + -0.7
4,311,880 + cysM OKFHMN_22170 0.35 +0.7
4,312,023 + cysM OKFHMN_22170 0.50 -0.1
4,312,063 - cysM OKFHMN_22170 0.55 +1.3
4,312,098 + cysM OKFHMN_22170 0.58 +2.0
4,312,314 - cysM OKFHMN_22170 0.82 -0.3
4,312,475 - -0.0
4,312,475 - -0.1
4,312,524 + +1.0
4,312,524 + -0.1
4,312,572 + yfeK OKFHMN_22175 0.17 +0.3
4,312,573 - yfeK OKFHMN_22175 0.18 -0.6
4,312,609 + yfeK OKFHMN_22175 0.29 +1.7
4,312,694 - yfeK OKFHMN_22175 0.54 +0.1
4,312,694 - yfeK OKFHMN_22175 0.54 -0.4
4,312,852 + +0.7
4,312,858 - +0.2
4,312,870 + +0.3
4,312,956 + +1.5
4,312,971 + -1.1
4,313,029 + +1.1
4,313,118 + pdxK OKFHMN_22180 0.20 +1.4
4,313,226 - pdxK OKFHMN_22180 0.32 -2.1
4,313,236 + pdxK OKFHMN_22180 0.33 -1.4
4,313,237 - pdxK OKFHMN_22180 0.34 +0.4
4,313,238 + pdxK OKFHMN_22180 0.34 -0.1
4,313,309 + pdxK OKFHMN_22180 0.42 +0.1

Or see this region's nucleotide sequence