Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfeX and pbp4b are separated by 57 nucleotides pbp4b and murP are separated by 4 nucleotides
OKFHMN_22120: yfeX - porphyrinogen peroxidase, at 4,300,931 to 4,301,830
yfeX
OKFHMN_22125: pbp4b - penicillin binding protein PBP4B, at 4,301,888 to 4,303,192
pbp4b
OKFHMN_22130: murP - PTS N-acetylmuramic acid transporter subunit IIBC, at 4,303,197 to 4,304,621
murP
Position (kb)
4301
4302
4303
4304 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4300.912 kb on - strand at 4301.111 kb on - strand, within yfeX at 4301.698 kb on + strand, within yfeX at 4301.792 kb on + strand at 4301.793 kb on - strand at 4301.887 kb on - strand at 4301.890 kb on - strand at 4301.911 kb on - strand at 4301.925 kb on - strand at 4301.935 kb on - strand at 4301.935 kb on - strand at 4301.935 kb on - strand at 4301.968 kb on + strand at 4301.969 kb on - strand at 4302.035 kb on - strand, within pbp4b at 4302.475 kb on + strand, within pbp4b at 4302.476 kb on - strand, within pbp4b at 4302.499 kb on - strand, within pbp4b at 4302.568 kb on + strand, within pbp4b at 4302.568 kb on + strand, within pbp4b at 4302.570 kb on + strand, within pbp4b at 4302.571 kb on - strand, within pbp4b at 4302.585 kb on + strand, within pbp4b at 4302.586 kb on - strand, within pbp4b at 4302.586 kb on - strand, within pbp4b at 4302.589 kb on + strand, within pbp4b at 4302.589 kb on + strand, within pbp4b at 4302.590 kb on - strand, within pbp4b at 4302.610 kb on - strand, within pbp4b at 4302.610 kb on - strand, within pbp4b at 4302.663 kb on + strand, within pbp4b at 4302.664 kb on - strand, within pbp4b at 4302.664 kb on - strand, within pbp4b at 4302.665 kb on + strand, within pbp4b at 4302.665 kb on + strand, within pbp4b at 4302.666 kb on - strand, within pbp4b at 4302.845 kb on + strand, within pbp4b at 4302.845 kb on + strand, within pbp4b at 4302.846 kb on - strand, within pbp4b at 4302.865 kb on + strand, within pbp4b at 4302.873 kb on + strand, within pbp4b at 4302.901 kb on - strand, within pbp4b at 4302.942 kb on - strand, within pbp4b at 4302.954 kb on + strand, within pbp4b at 4302.954 kb on + strand, within pbp4b at 4303.005 kb on - strand, within pbp4b at 4303.051 kb on + strand, within pbp4b at 4303.147 kb on + strand at 4303.147 kb on + strand at 4303.148 kb on - strand at 4303.154 kb on - strand at 4303.173 kb on + strand at 4303.174 kb on - strand at 4303.195 kb on + strand at 4303.196 kb on - strand at 4303.280 kb on - strand at 4303.362 kb on + strand, within murP at 4303.439 kb on + strand, within murP at 4303.509 kb on - strand, within murP at 4303.664 kb on + strand, within murP at 4303.664 kb on + strand, within murP at 4303.737 kb on - strand, within murP at 4303.752 kb on - strand, within murP at 4303.766 kb on + strand, within murP at 4303.796 kb on + strand, within murP at 4303.808 kb on - strand, within murP at 4303.830 kb on + strand, within murP at 4303.874 kb on + strand, within murP at 4303.938 kb on - strand, within murP at 4303.988 kb on - strand, within murP at 4304.039 kb on + strand, within murP at 4304.039 kb on + strand, within murP at 4304.086 kb on + strand, within murP at 4304.086 kb on + strand, within murP
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,300,912 - +1.0 4,301,111 - yfeX OKFHMN_22120 0.20 +0.5 4,301,698 + yfeX OKFHMN_22120 0.85 +0.3 4,301,792 + +0.2 4,301,793 - -0.1 4,301,887 - -2.4 4,301,890 - +0.2 4,301,911 - +0.5 4,301,925 - +1.8 4,301,935 - +0.0 4,301,935 - -0.2 4,301,935 - +0.0 4,301,968 + +0.0 4,301,969 - -0.5 4,302,035 - pbp4b OKFHMN_22125 0.11 -0.1 4,302,475 + pbp4b OKFHMN_22125 0.45 -0.9 4,302,476 - pbp4b OKFHMN_22125 0.45 +1.5 4,302,499 - pbp4b OKFHMN_22125 0.47 -0.2 4,302,568 + pbp4b OKFHMN_22125 0.52 -1.6 4,302,568 + pbp4b OKFHMN_22125 0.52 -0.0 4,302,570 + pbp4b OKFHMN_22125 0.52 -0.2 4,302,571 - pbp4b OKFHMN_22125 0.52 -0.8 4,302,585 + pbp4b OKFHMN_22125 0.53 +0.2 4,302,586 - pbp4b OKFHMN_22125 0.53 +0.2 4,302,586 - pbp4b OKFHMN_22125 0.53 +0.0 4,302,589 + pbp4b OKFHMN_22125 0.54 +0.5 4,302,589 + pbp4b OKFHMN_22125 0.54 -0.6 4,302,590 - pbp4b OKFHMN_22125 0.54 +0.6 4,302,610 - pbp4b OKFHMN_22125 0.55 +0.4 4,302,610 - pbp4b OKFHMN_22125 0.55 +0.5 4,302,663 + pbp4b OKFHMN_22125 0.59 -2.5 4,302,664 - pbp4b OKFHMN_22125 0.59 -0.1 4,302,664 - pbp4b OKFHMN_22125 0.59 -2.0 4,302,665 + pbp4b OKFHMN_22125 0.60 +0.4 4,302,665 + pbp4b OKFHMN_22125 0.60 +0.5 4,302,666 - pbp4b OKFHMN_22125 0.60 -0.2 4,302,845 + pbp4b OKFHMN_22125 0.73 +0.1 4,302,845 + pbp4b OKFHMN_22125 0.73 +2.3 4,302,846 - pbp4b OKFHMN_22125 0.73 +1.1 4,302,865 + pbp4b OKFHMN_22125 0.75 -0.8 4,302,873 + pbp4b OKFHMN_22125 0.75 -0.3 4,302,901 - pbp4b OKFHMN_22125 0.78 -0.6 4,302,942 - pbp4b OKFHMN_22125 0.81 +1.1 4,302,954 + pbp4b OKFHMN_22125 0.82 +0.1 4,302,954 + pbp4b OKFHMN_22125 0.82 -0.0 4,303,005 - pbp4b OKFHMN_22125 0.86 -0.6 4,303,051 + pbp4b OKFHMN_22125 0.89 -1.0 4,303,147 + +0.4 4,303,147 + +0.6 4,303,148 - +1.2 4,303,154 - +0.6 4,303,173 + -0.9 4,303,174 - -0.1 4,303,195 + -1.5 4,303,196 - +0.9 4,303,280 - +0.4 4,303,362 + murP OKFHMN_22130 0.12 -3.4 4,303,439 + murP OKFHMN_22130 0.17 -0.0 4,303,509 - murP OKFHMN_22130 0.22 +0.5 4,303,664 + murP OKFHMN_22130 0.33 -0.3 4,303,664 + murP OKFHMN_22130 0.33 +0.1 4,303,737 - murP OKFHMN_22130 0.38 +1.9 4,303,752 - murP OKFHMN_22130 0.39 -1.6 4,303,766 + murP OKFHMN_22130 0.40 +0.6 4,303,796 + murP OKFHMN_22130 0.42 +0.1 4,303,808 - murP OKFHMN_22130 0.43 -0.1 4,303,830 + murP OKFHMN_22130 0.44 -0.3 4,303,874 + murP OKFHMN_22130 0.48 -0.2 4,303,938 - murP OKFHMN_22130 0.52 -2.6 4,303,988 - murP OKFHMN_22130 0.56 +0.8 4,304,039 + murP OKFHMN_22130 0.59 +0.1 4,304,039 + murP OKFHMN_22130 0.59 +0.4 4,304,086 + murP OKFHMN_22130 0.62 -2.9 4,304,086 + murP OKFHMN_22130 0.62 -1.0
Or see this region's nucleotide sequence