Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eutQ and eutT overlap by 4 nucleotides eutT and pta overlap by 4 nucleotides pta and eutM overlap by 4 nucleotides eutM and eutN are separated by 106 nucleotides eutN and eutE are separated by 11 nucleotides
OKFHMN_22020: eutQ - ethanolamine utilization acetate kinase EutQ, at 4,283,452 to 4,284,153
eutQ
OKFHMN_22025: eutT - ethanolamine utilization cob(I)yrinic acid a,c-diamide adenosyltransferase EutT, at 4,284,150 to 4,284,881
eutT
OKFHMN_22030: pta - phosphate acetyltransferase, at 4,284,878 to 4,285,894
pta
OKFHMN_22035: eutM - ethanolamine utilization microcompartment protein EutM, at 4,285,891 to 4,286,226
eutM
OKFHMN_22040: eutN - ethanolamine utilization microcompartment protein EutN, at 4,286,333 to 4,286,620
eutN
OKFHMN_22045: eutE - Acetaldehyde dehydrogenase (acetylating) EutE, at 4,286,632 to 4,288,035
eutE
Position (kb) 4284
4285
4286 Strain fitness (log2 ratio) -2
-1
0
1 at 4284.290 kb on + strand, within eutT at 4284.425 kb on + strand, within eutT at 4284.801 kb on + strand, within eutT at 4284.802 kb on - strand, within eutT at 4284.824 kb on - strand at 4284.945 kb on + strand at 4285.072 kb on - strand, within pta at 4285.309 kb on + strand, within pta at 4285.386 kb on + strand, within pta at 4285.576 kb on - strand, within pta at 4285.755 kb on + strand, within pta at 4285.756 kb on - strand, within pta at 4285.798 kb on + strand at 4285.799 kb on - strand at 4286.056 kb on - strand, within eutM at 4286.147 kb on + strand, within eutM at 4286.147 kb on + strand, within eutM at 4286.148 kb on - strand, within eutM at 4286.224 kb on + strand at 4286.374 kb on - strand, within eutN at 4286.470 kb on + strand, within eutN at 4286.618 kb on + strand at 4286.646 kb on + strand at 4286.805 kb on - strand, within eutE
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,284,290 + eutT OKFHMN_22025 0.19 +0.8 4,284,425 + eutT OKFHMN_22025 0.38 -0.2 4,284,801 + eutT OKFHMN_22025 0.89 +0.4 4,284,802 - eutT OKFHMN_22025 0.89 -0.7 4,284,824 - +0.1 4,284,945 + +0.8 4,285,072 - pta OKFHMN_22030 0.19 +0.0 4,285,309 + pta OKFHMN_22030 0.42 -0.9 4,285,386 + pta OKFHMN_22030 0.50 -0.0 4,285,576 - pta OKFHMN_22030 0.69 +0.9 4,285,755 + pta OKFHMN_22030 0.86 -0.1 4,285,756 - pta OKFHMN_22030 0.86 +0.2 4,285,798 + +0.2 4,285,799 - -0.9 4,286,056 - eutM OKFHMN_22035 0.49 +0.3 4,286,147 + eutM OKFHMN_22035 0.76 +0.1 4,286,147 + eutM OKFHMN_22035 0.76 +0.4 4,286,148 - eutM OKFHMN_22035 0.76 +0.8 4,286,224 + -2.5 4,286,374 - eutN OKFHMN_22040 0.14 +1.4 4,286,470 + eutN OKFHMN_22040 0.48 +0.4 4,286,618 + -0.4 4,286,646 + -1.6 4,286,805 - eutE OKFHMN_22045 0.12 +0.2
Or see this region's nucleotide sequence