Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bamC and OKFHMN_21925 overlap by 90 nucleotides OKFHMN_21925 and purC are separated by 71 nucleotides purC and ypfJ are separated by 167 nucleotides
OKFHMN_21920: bamC - outer membrane protein assembly factor BamC, at 4,259,619 to 4,260,653
bamC
OKFHMN_21925: OKFHMN_21925 - hypothetical protein, at 4,260,564 to 4,260,794
_21925
OKFHMN_21930: purC - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 4,260,866 to 4,261,579
purC
OKFHMN_21935: ypfJ - Uncharacterized protein YpfJ, at 4,261,747 to 4,262,610
ypfJ
Position (kb) 4260
4261
4262 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 4260.087 kb on + strand, within bamC at 4260.207 kb on + strand, within bamC at 4260.230 kb on + strand, within bamC at 4260.299 kb on + strand, within bamC at 4260.307 kb on + strand, within bamC at 4260.456 kb on + strand, within bamC at 4260.456 kb on + strand, within bamC at 4260.457 kb on - strand, within bamC at 4260.457 kb on - strand, within bamC at 4260.459 kb on - strand, within bamC at 4260.526 kb on - strand, within bamC at 4260.527 kb on + strand, within bamC at 4260.590 kb on + strand at 4260.622 kb on + strand at 4260.767 kb on + strand, within OKFHMN_21925 at 4260.842 kb on - strand at 4260.888 kb on - strand at 4260.911 kb on + strand at 4260.911 kb on + strand at 4260.911 kb on + strand at 4260.912 kb on - strand at 4260.958 kb on + strand, within purC at 4260.959 kb on - strand, within purC at 4261.006 kb on + strand, within purC at 4261.028 kb on + strand, within purC at 4261.196 kb on - strand, within purC at 4261.331 kb on + strand, within purC at 4261.412 kb on + strand, within purC at 4261.412 kb on + strand, within purC at 4261.539 kb on - strand at 4261.566 kb on + strand at 4261.567 kb on - strand at 4261.567 kb on - strand at 4261.688 kb on + strand at 4261.689 kb on - strand at 4261.729 kb on + strand at 4261.730 kb on - strand at 4261.732 kb on - strand at 4261.732 kb on - strand at 4261.743 kb on - strand at 4261.747 kb on - strand at 4261.797 kb on + strand at 4261.798 kb on - strand at 4261.894 kb on + strand, within ypfJ at 4261.894 kb on + strand, within ypfJ at 4262.052 kb on + strand, within ypfJ at 4262.083 kb on + strand, within ypfJ at 4262.083 kb on + strand, within ypfJ at 4262.084 kb on - strand, within ypfJ at 4262.141 kb on - strand, within ypfJ at 4262.183 kb on - strand, within ypfJ at 4262.233 kb on + strand, within ypfJ at 4262.340 kb on - strand, within ypfJ at 4262.391 kb on + strand, within ypfJ at 4262.392 kb on - strand, within ypfJ at 4262.392 kb on - strand, within ypfJ at 4262.499 kb on - strand, within ypfJ at 4262.540 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,260,087 + bamC OKFHMN_21920 0.45 +0.4 4,260,207 + bamC OKFHMN_21920 0.57 +0.3 4,260,230 + bamC OKFHMN_21920 0.59 -0.1 4,260,299 + bamC OKFHMN_21920 0.66 +0.6 4,260,307 + bamC OKFHMN_21920 0.66 +0.2 4,260,456 + bamC OKFHMN_21920 0.81 +0.3 4,260,456 + bamC OKFHMN_21920 0.81 +0.9 4,260,457 - bamC OKFHMN_21920 0.81 -0.4 4,260,457 - bamC OKFHMN_21920 0.81 -1.0 4,260,459 - bamC OKFHMN_21920 0.81 +0.8 4,260,526 - bamC OKFHMN_21920 0.88 -0.5 4,260,527 + bamC OKFHMN_21920 0.88 +0.3 4,260,590 + +0.4 4,260,622 + +0.1 4,260,767 + OKFHMN_21925 0.88 +1.4 4,260,842 - -5.0 4,260,888 - -4.2 4,260,911 + -3.5 4,260,911 + -4.2 4,260,911 + -6.3 4,260,912 - -5.4 4,260,958 + purC OKFHMN_21930 0.13 -5.4 4,260,959 - purC OKFHMN_21930 0.13 -3.4 4,261,006 + purC OKFHMN_21930 0.20 -1.2 4,261,028 + purC OKFHMN_21930 0.23 -3.5 4,261,196 - purC OKFHMN_21930 0.46 -0.6 4,261,331 + purC OKFHMN_21930 0.65 -3.2 4,261,412 + purC OKFHMN_21930 0.76 -3.9 4,261,412 + purC OKFHMN_21930 0.76 -4.8 4,261,539 - -4.8 4,261,566 + -0.0 4,261,567 - -3.4 4,261,567 - -4.6 4,261,688 + +0.1 4,261,689 - +0.0 4,261,729 + +0.9 4,261,730 - -0.2 4,261,732 - -1.0 4,261,732 - -2.3 4,261,743 - +0.4 4,261,747 - -0.5 4,261,797 + -2.5 4,261,798 - +1.1 4,261,894 + ypfJ OKFHMN_21935 0.17 +0.1 4,261,894 + ypfJ OKFHMN_21935 0.17 +2.2 4,262,052 + ypfJ OKFHMN_21935 0.35 -0.5 4,262,083 + ypfJ OKFHMN_21935 0.39 -0.7 4,262,083 + ypfJ OKFHMN_21935 0.39 +0.0 4,262,084 - ypfJ OKFHMN_21935 0.39 +0.3 4,262,141 - ypfJ OKFHMN_21935 0.46 +0.3 4,262,183 - ypfJ OKFHMN_21935 0.50 -2.1 4,262,233 + ypfJ OKFHMN_21935 0.56 +0.0 4,262,340 - ypfJ OKFHMN_21935 0.69 +0.2 4,262,391 + ypfJ OKFHMN_21935 0.75 +0.2 4,262,392 - ypfJ OKFHMN_21935 0.75 +1.0 4,262,392 - ypfJ OKFHMN_21935 0.75 -0.1 4,262,499 - ypfJ OKFHMN_21935 0.87 -1.7 4,262,540 - +0.5
Or see this region's nucleotide sequence