Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hypD and hypC overlap by 1 nucleotides hypC and hypB overlap by 10 nucleotides hypB and hypA are separated by 3 nucleotides
OKFHMN_20550: hypD - hydrogenase formation protein HypD, at 3,999,449 to 4,000,570
hypD
OKFHMN_20555: hypC - hydrogenase 3 maturation protein HypC, at 4,000,570 to 4,000,842
hypC
OKFHMN_20560: hypB - hydrogenase nickel incorporation protein HypB, at 4,000,833 to 4,001,705
hypB
OKFHMN_20565: hypA - hydrogenase maturation nickel metallochaperone HypA, at 4,001,709 to 4,002,059
hypA
Position (kb) 4000
4001 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3999.821 kb on + strand, within hypD at 3999.962 kb on - strand, within hypD at 3999.964 kb on + strand, within hypD at 3999.964 kb on + strand, within hypD at 3999.965 kb on - strand, within hypD at 4000.042 kb on + strand, within hypD at 4000.042 kb on + strand, within hypD at 4000.043 kb on - strand, within hypD at 4000.045 kb on + strand, within hypD at 4000.045 kb on + strand, within hypD at 4000.046 kb on - strand, within hypD at 4000.046 kb on - strand, within hypD at 4000.046 kb on - strand, within hypD at 4000.046 kb on - strand, within hypD at 4000.289 kb on - strand, within hypD at 4000.439 kb on - strand, within hypD at 4000.586 kb on + strand at 4000.666 kb on - strand, within hypC at 4000.670 kb on - strand, within hypC at 4000.693 kb on + strand, within hypC at 4000.694 kb on - strand, within hypC at 4000.700 kb on - strand, within hypC at 4000.754 kb on - strand, within hypC at 4001.147 kb on + strand, within hypB at 4001.331 kb on + strand, within hypB at 4001.441 kb on + strand, within hypB at 4001.527 kb on + strand, within hypB at 4001.668 kb on + strand at 4001.668 kb on + strand at 4001.699 kb on + strand at 4001.824 kb on + strand, within hypA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,999,821 + hypD OKFHMN_20550 0.33 +0.8 3,999,962 - hypD OKFHMN_20550 0.46 -0.1 3,999,964 + hypD OKFHMN_20550 0.46 +0.2 3,999,964 + hypD OKFHMN_20550 0.46 -0.4 3,999,965 - hypD OKFHMN_20550 0.46 -0.3 4,000,042 + hypD OKFHMN_20550 0.53 -0.4 4,000,042 + hypD OKFHMN_20550 0.53 -3.1 4,000,043 - hypD OKFHMN_20550 0.53 +0.0 4,000,045 + hypD OKFHMN_20550 0.53 -0.3 4,000,045 + hypD OKFHMN_20550 0.53 -0.2 4,000,046 - hypD OKFHMN_20550 0.53 -0.1 4,000,046 - hypD OKFHMN_20550 0.53 -1.2 4,000,046 - hypD OKFHMN_20550 0.53 -0.4 4,000,046 - hypD OKFHMN_20550 0.53 +0.2 4,000,289 - hypD OKFHMN_20550 0.75 -1.1 4,000,439 - hypD OKFHMN_20550 0.88 +0.6 4,000,586 + +0.4 4,000,666 - hypC OKFHMN_20555 0.35 +0.0 4,000,670 - hypC OKFHMN_20555 0.37 -0.0 4,000,693 + hypC OKFHMN_20555 0.45 +0.8 4,000,694 - hypC OKFHMN_20555 0.45 +0.2 4,000,700 - hypC OKFHMN_20555 0.48 -1.3 4,000,754 - hypC OKFHMN_20555 0.67 -0.2 4,001,147 + hypB OKFHMN_20560 0.36 +0.5 4,001,331 + hypB OKFHMN_20560 0.57 +0.6 4,001,441 + hypB OKFHMN_20560 0.70 -0.4 4,001,527 + hypB OKFHMN_20560 0.79 -0.6 4,001,668 + +0.7 4,001,668 + +0.6 4,001,699 + +1.0 4,001,824 + hypA OKFHMN_20565 0.33 -0.0
Or see this region's nucleotide sequence