Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cas7e and cas5e are separated by 10 nucleotides cas5e and cas6e overlap by 19 nucleotides cas6e and cas1e overlap by 4 nucleotides
OKFHMN_20410: cas7e - type I-E CRISPR-associated protein Cas7/Cse4/CasC, at 3,974,204 to 3,975,259
cas7e
OKFHMN_20415: cas5e - type I-E CRISPR-associated protein Cas5/CasD, at 3,975,270 to 3,976,016
cas5e
OKFHMN_20420: cas6e - type I-E CRISPR-associated protein Cas6/Cse3/CasE, at 3,975,998 to 3,976,648
cas6e
OKFHMN_20425: cas1e - type I-E CRISPR-associated endonuclease Cas1e, at 3,976,645 to 3,977,568
cas1e
Position (kb)
3975
3976
3977 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3974.387 kb on - strand, within cas7e at 3974.407 kb on + strand, within cas7e at 3974.428 kb on + strand, within cas7e at 3974.429 kb on - strand, within cas7e at 3974.559 kb on + strand, within cas7e at 3974.559 kb on + strand, within cas7e at 3974.566 kb on + strand, within cas7e at 3974.592 kb on + strand, within cas7e at 3974.663 kb on - strand, within cas7e at 3974.679 kb on + strand, within cas7e at 3974.680 kb on - strand, within cas7e at 3974.680 kb on - strand, within cas7e at 3974.689 kb on + strand, within cas7e at 3974.690 kb on - strand, within cas7e at 3974.702 kb on - strand, within cas7e at 3974.702 kb on - strand, within cas7e at 3974.713 kb on + strand, within cas7e at 3974.725 kb on + strand, within cas7e at 3974.882 kb on + strand, within cas7e at 3974.882 kb on + strand, within cas7e at 3974.883 kb on - strand, within cas7e at 3974.888 kb on + strand, within cas7e at 3974.888 kb on + strand, within cas7e at 3974.889 kb on - strand, within cas7e at 3974.891 kb on - strand, within cas7e at 3974.903 kb on - strand, within cas7e at 3974.947 kb on + strand, within cas7e at 3974.983 kb on + strand, within cas7e at 3975.029 kb on + strand, within cas7e at 3975.120 kb on + strand, within cas7e at 3975.140 kb on - strand, within cas7e at 3975.140 kb on - strand, within cas7e at 3975.142 kb on + strand, within cas7e at 3975.143 kb on - strand, within cas7e at 3975.257 kb on + strand at 3975.430 kb on + strand, within cas5e at 3975.498 kb on + strand, within cas5e at 3975.530 kb on + strand, within cas5e at 3975.530 kb on + strand, within cas5e at 3975.688 kb on + strand, within cas5e at 3975.787 kb on + strand, within cas5e at 3975.788 kb on - strand, within cas5e at 3975.792 kb on - strand, within cas5e at 3975.798 kb on + strand, within cas5e at 3976.001 kb on + strand at 3976.001 kb on + strand at 3976.001 kb on + strand at 3976.002 kb on - strand at 3976.015 kb on - strand at 3976.020 kb on + strand at 3976.020 kb on + strand at 3976.020 kb on + strand at 3976.020 kb on + strand at 3976.021 kb on - strand at 3976.024 kb on + strand at 3976.025 kb on - strand at 3976.076 kb on + strand, within cas6e at 3976.077 kb on - strand, within cas6e at 3976.541 kb on - strand, within cas6e at 3976.655 kb on + strand at 3976.693 kb on - strand at 3976.724 kb on + strand at 3976.935 kb on + strand, within cas1e at 3976.941 kb on - strand, within cas1e at 3976.945 kb on + strand, within cas1e at 3977.002 kb on + strand, within cas1e
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,974,387 - cas7e OKFHMN_20410 0.17 -0.1 3,974,407 + cas7e OKFHMN_20410 0.19 +0.3 3,974,428 + cas7e OKFHMN_20410 0.21 -1.4 3,974,429 - cas7e OKFHMN_20410 0.21 -0.4 3,974,559 + cas7e OKFHMN_20410 0.34 -0.0 3,974,559 + cas7e OKFHMN_20410 0.34 -0.3 3,974,566 + cas7e OKFHMN_20410 0.34 +0.4 3,974,592 + cas7e OKFHMN_20410 0.37 +0.7 3,974,663 - cas7e OKFHMN_20410 0.43 +0.4 3,974,679 + cas7e OKFHMN_20410 0.45 -0.2 3,974,680 - cas7e OKFHMN_20410 0.45 +0.1 3,974,680 - cas7e OKFHMN_20410 0.45 +0.2 3,974,689 + cas7e OKFHMN_20410 0.46 -0.2 3,974,690 - cas7e OKFHMN_20410 0.46 -0.2 3,974,702 - cas7e OKFHMN_20410 0.47 -1.9 3,974,702 - cas7e OKFHMN_20410 0.47 +0.1 3,974,713 + cas7e OKFHMN_20410 0.48 +0.2 3,974,725 + cas7e OKFHMN_20410 0.49 +0.1 3,974,882 + cas7e OKFHMN_20410 0.64 +0.2 3,974,882 + cas7e OKFHMN_20410 0.64 +2.5 3,974,883 - cas7e OKFHMN_20410 0.64 -0.7 3,974,888 + cas7e OKFHMN_20410 0.65 -0.1 3,974,888 + cas7e OKFHMN_20410 0.65 -0.3 3,974,889 - cas7e OKFHMN_20410 0.65 +0.8 3,974,891 - cas7e OKFHMN_20410 0.65 +1.1 3,974,903 - cas7e OKFHMN_20410 0.66 +0.2 3,974,947 + cas7e OKFHMN_20410 0.70 -0.3 3,974,983 + cas7e OKFHMN_20410 0.74 +0.6 3,975,029 + cas7e OKFHMN_20410 0.78 +0.2 3,975,120 + cas7e OKFHMN_20410 0.87 -2.8 3,975,140 - cas7e OKFHMN_20410 0.89 +0.3 3,975,140 - cas7e OKFHMN_20410 0.89 +0.2 3,975,142 + cas7e OKFHMN_20410 0.89 +0.8 3,975,143 - cas7e OKFHMN_20410 0.89 -0.5 3,975,257 + -0.5 3,975,430 + cas5e OKFHMN_20415 0.21 +0.8 3,975,498 + cas5e OKFHMN_20415 0.31 +0.9 3,975,530 + cas5e OKFHMN_20415 0.35 -0.0 3,975,530 + cas5e OKFHMN_20415 0.35 +0.6 3,975,688 + cas5e OKFHMN_20415 0.56 +0.9 3,975,787 + cas5e OKFHMN_20415 0.69 -1.1 3,975,788 - cas5e OKFHMN_20415 0.69 +0.5 3,975,792 - cas5e OKFHMN_20415 0.70 -0.7 3,975,798 + cas5e OKFHMN_20415 0.71 +1.4 3,976,001 + -1.9 3,976,001 + -0.1 3,976,001 + -0.4 3,976,002 - +0.5 3,976,015 - -0.2 3,976,020 + +1.3 3,976,020 + -0.1 3,976,020 + +0.1 3,976,020 + -0.3 3,976,021 - -0.1 3,976,024 + +0.8 3,976,025 - -0.6 3,976,076 + cas6e OKFHMN_20420 0.12 +0.8 3,976,077 - cas6e OKFHMN_20420 0.12 +0.3 3,976,541 - cas6e OKFHMN_20420 0.83 +0.2 3,976,655 + +1.9 3,976,693 - -0.1 3,976,724 + +0.3 3,976,935 + cas1e OKFHMN_20425 0.31 -0.2 3,976,941 - cas1e OKFHMN_20425 0.32 +0.1 3,976,945 + cas1e OKFHMN_20425 0.32 -0.4 3,977,002 + cas1e OKFHMN_20425 0.39 +0.3
Or see this region's nucleotide sequence