Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygcU and ygcS are separated by 21 nucleotides ygcS and ygcS overlap by 4 nucleotides ygcS and ygcR overlap by 23 nucleotides ygcR and fixB overlap by 4 nucleotides
OKFHMN_20320: ygcU - Uncharacterized FAD-linked oxidoreductase YgcU, at 3,956,854 to 3,958,308
ygcU
OKFHMN_20325: ygcS - Inner membrane metabolite transport protein YgcS, at 3,958,330 to 3,959,535
ygcS
OKFHMN_20330: ygcS - Inner membrane metabolite transport protein YgcS, at 3,959,532 to 3,959,738
ygcS
OKFHMN_20335: ygcR - Putative electron transfer flavoprotein subunit YgcR, at 3,959,716 to 3,960,495
ygcR
OKFHMN_20340: fixB - electron transfer flavoprotein, at 3,960,492 to 3,961,061
fixB
Position (kb)
3958
3959
3960 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3957.497 kb on - strand, within ygcU at 3957.553 kb on + strand, within ygcU at 3957.559 kb on + strand, within ygcU at 3957.941 kb on - strand, within ygcU at 3958.020 kb on + strand, within ygcU at 3958.020 kb on + strand, within ygcU at 3958.021 kb on - strand, within ygcU at 3958.021 kb on - strand, within ygcU at 3958.021 kb on - strand, within ygcU at 3958.021 kb on - strand, within ygcU at 3958.045 kb on + strand, within ygcU at 3958.045 kb on + strand, within ygcU at 3958.045 kb on + strand, within ygcU at 3958.060 kb on + strand, within ygcU at 3958.077 kb on + strand, within ygcU at 3958.102 kb on + strand, within ygcU at 3958.103 kb on - strand, within ygcU at 3958.272 kb on + strand at 3958.287 kb on + strand at 3958.287 kb on + strand at 3958.288 kb on - strand at 3958.498 kb on + strand, within ygcS at 3958.499 kb on - strand, within ygcS at 3958.499 kb on - strand, within ygcS at 3958.527 kb on + strand, within ygcS at 3958.775 kb on - strand, within ygcS at 3958.781 kb on + strand, within ygcS at 3958.781 kb on + strand, within ygcS at 3958.862 kb on + strand, within ygcS at 3958.867 kb on + strand, within ygcS at 3958.867 kb on + strand, within ygcS at 3958.868 kb on - strand, within ygcS at 3958.868 kb on - strand, within ygcS at 3958.868 kb on - strand, within ygcS at 3958.966 kb on - strand, within ygcS at 3958.966 kb on - strand, within ygcS at 3959.104 kb on - strand, within ygcS at 3959.229 kb on + strand, within ygcS at 3959.229 kb on + strand, within ygcS at 3959.229 kb on + strand, within ygcS at 3959.268 kb on + strand, within ygcS at 3959.304 kb on - strand, within ygcS at 3959.375 kb on + strand, within ygcS at 3959.456 kb on + strand at 3959.608 kb on + strand, within ygcS at 3959.647 kb on + strand, within ygcS at 3959.647 kb on + strand, within ygcS at 3959.982 kb on - strand, within ygcR at 3960.210 kb on + strand, within ygcR at 3960.210 kb on + strand, within ygcR at 3960.458 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,957,497 - ygcU OKFHMN_20320 0.44 +0.0 3,957,553 + ygcU OKFHMN_20320 0.48 -0.1 3,957,559 + ygcU OKFHMN_20320 0.48 +0.5 3,957,941 - ygcU OKFHMN_20320 0.75 -0.2 3,958,020 + ygcU OKFHMN_20320 0.80 -0.5 3,958,020 + ygcU OKFHMN_20320 0.80 +0.3 3,958,021 - ygcU OKFHMN_20320 0.80 -0.1 3,958,021 - ygcU OKFHMN_20320 0.80 +2.1 3,958,021 - ygcU OKFHMN_20320 0.80 +0.2 3,958,021 - ygcU OKFHMN_20320 0.80 +0.4 3,958,045 + ygcU OKFHMN_20320 0.82 +0.3 3,958,045 + ygcU OKFHMN_20320 0.82 -0.2 3,958,045 + ygcU OKFHMN_20320 0.82 -0.5 3,958,060 + ygcU OKFHMN_20320 0.83 +0.1 3,958,077 + ygcU OKFHMN_20320 0.84 +1.1 3,958,102 + ygcU OKFHMN_20320 0.86 +0.6 3,958,103 - ygcU OKFHMN_20320 0.86 +0.4 3,958,272 + -0.6 3,958,287 + +0.2 3,958,287 + -1.8 3,958,288 - -2.0 3,958,498 + ygcS OKFHMN_20325 0.14 +0.4 3,958,499 - ygcS OKFHMN_20325 0.14 +0.2 3,958,499 - ygcS OKFHMN_20325 0.14 +0.3 3,958,527 + ygcS OKFHMN_20325 0.16 +0.4 3,958,775 - ygcS OKFHMN_20325 0.37 -1.1 3,958,781 + ygcS OKFHMN_20325 0.37 -0.2 3,958,781 + ygcS OKFHMN_20325 0.37 -0.3 3,958,862 + ygcS OKFHMN_20325 0.44 +0.4 3,958,867 + ygcS OKFHMN_20325 0.45 +0.3 3,958,867 + ygcS OKFHMN_20325 0.45 -2.3 3,958,868 - ygcS OKFHMN_20325 0.45 +0.6 3,958,868 - ygcS OKFHMN_20325 0.45 -1.3 3,958,868 - ygcS OKFHMN_20325 0.45 +0.2 3,958,966 - ygcS OKFHMN_20325 0.53 -1.7 3,958,966 - ygcS OKFHMN_20325 0.53 -0.2 3,959,104 - ygcS OKFHMN_20325 0.64 -0.4 3,959,229 + ygcS OKFHMN_20325 0.75 -1.7 3,959,229 + ygcS OKFHMN_20325 0.75 +0.3 3,959,229 + ygcS OKFHMN_20325 0.75 +0.5 3,959,268 + ygcS OKFHMN_20325 0.78 +0.4 3,959,304 - ygcS OKFHMN_20325 0.81 +0.1 3,959,375 + ygcS OKFHMN_20325 0.87 +0.1 3,959,456 + -0.7 3,959,608 + ygcS OKFHMN_20330 0.37 -0.0 3,959,647 + ygcS OKFHMN_20330 0.56 +0.4 3,959,647 + ygcS OKFHMN_20330 0.56 -0.4 3,959,982 - ygcR OKFHMN_20335 0.34 +0.5 3,960,210 + ygcR OKFHMN_20335 0.63 +0.9 3,960,210 + ygcR OKFHMN_20335 0.63 +0.3 3,960,458 + -0.6
Or see this region's nucleotide sequence