Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygdT and rppH are separated by 352 nucleotides rppH and ptsP are separated by 12 nucleotides ptsP and lgt are separated by 150 nucleotides
OKFHMN_19995: ygdT - Protein YgdT, at 3,884,514 to 3,884,660
ygdT
OKFHMN_20000: rppH - RNA pyrophosphohydrolase, at 3,885,013 to 3,885,543
rppH
OKFHMN_20005: ptsP - phosphoenolpyruvate--protein phosphotransferase, at 3,885,556 to 3,887,802
ptsP
OKFHMN_20010: lgt - prolipoprotein diacylglyceryl transferase, at 3,887,953 to 3,888,828
lgt
Position (kb)
3885
3886
3887
3888 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3884.571 kb on - strand, within ygdT at 3884.771 kb on - strand at 3884.810 kb on + strand at 3884.929 kb on + strand at 3884.930 kb on - strand at 3884.952 kb on - strand at 3884.985 kb on - strand at 3885.077 kb on + strand, within rppH at 3885.397 kb on + strand, within rppH at 3885.532 kb on - strand at 3885.555 kb on + strand at 3885.555 kb on + strand at 3885.556 kb on - strand at 3885.556 kb on - strand at 3885.620 kb on + strand at 3885.621 kb on - strand at 3885.703 kb on + strand at 3885.703 kb on + strand at 3885.706 kb on + strand at 3885.707 kb on - strand at 3885.752 kb on + strand at 3885.753 kb on - strand at 3885.847 kb on + strand, within ptsP at 3885.847 kb on + strand, within ptsP at 3885.847 kb on + strand, within ptsP at 3885.848 kb on - strand, within ptsP at 3885.848 kb on - strand, within ptsP at 3885.848 kb on - strand, within ptsP at 3885.893 kb on + strand, within ptsP at 3885.893 kb on + strand, within ptsP at 3885.900 kb on + strand, within ptsP at 3885.900 kb on + strand, within ptsP at 3885.901 kb on - strand, within ptsP at 3885.926 kb on + strand, within ptsP at 3885.935 kb on + strand, within ptsP at 3886.095 kb on - strand, within ptsP at 3886.128 kb on - strand, within ptsP at 3886.134 kb on - strand, within ptsP at 3886.371 kb on - strand, within ptsP at 3886.420 kb on - strand, within ptsP at 3886.423 kb on + strand, within ptsP at 3886.423 kb on + strand, within ptsP at 3886.423 kb on + strand, within ptsP at 3886.448 kb on + strand, within ptsP at 3886.449 kb on - strand, within ptsP at 3886.584 kb on - strand, within ptsP at 3886.637 kb on + strand, within ptsP at 3886.860 kb on - strand, within ptsP at 3886.883 kb on + strand, within ptsP at 3886.884 kb on - strand, within ptsP at 3886.884 kb on - strand, within ptsP at 3887.070 kb on + strand, within ptsP at 3887.070 kb on + strand, within ptsP at 3887.083 kb on + strand, within ptsP at 3887.083 kb on + strand, within ptsP at 3887.083 kb on + strand, within ptsP at 3887.084 kb on - strand, within ptsP at 3887.084 kb on - strand, within ptsP at 3887.365 kb on - strand, within ptsP at 3887.365 kb on - strand, within ptsP at 3887.474 kb on - strand, within ptsP at 3887.498 kb on + strand, within ptsP at 3887.499 kb on - strand, within ptsP at 3887.534 kb on + strand, within ptsP at 3887.625 kb on + strand at 3887.657 kb on - strand at 3887.657 kb on - strand at 3887.678 kb on - strand at 3887.707 kb on - strand at 3887.707 kb on - strand at 3887.800 kb on + strand at 3887.800 kb on + strand at 3887.812 kb on + strand at 3887.812 kb on + strand at 3887.813 kb on - strand at 3887.814 kb on + strand at 3887.814 kb on + strand at 3887.815 kb on - strand at 3887.815 kb on - strand at 3887.818 kb on + strand at 3887.818 kb on + strand at 3887.818 kb on + strand at 3887.819 kb on - strand at 3887.819 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,884,571 - ygdT OKFHMN_19995 0.39 +0.7 3,884,771 - +0.2 3,884,810 + -0.4 3,884,929 + +0.3 3,884,930 - +0.6 3,884,952 - -0.7 3,884,985 - +0.0 3,885,077 + rppH OKFHMN_20000 0.12 -0.4 3,885,397 + rppH OKFHMN_20000 0.72 -2.0 3,885,532 - -0.2 3,885,555 + -0.1 3,885,555 + -1.0 3,885,556 - -0.7 3,885,556 - -0.2 3,885,620 + +0.1 3,885,621 - -0.4 3,885,703 + -0.2 3,885,703 + +2.1 3,885,706 + +0.0 3,885,707 - -0.2 3,885,752 + +0.3 3,885,753 - -0.1 3,885,847 + ptsP OKFHMN_20005 0.13 -0.1 3,885,847 + ptsP OKFHMN_20005 0.13 -1.1 3,885,847 + ptsP OKFHMN_20005 0.13 -0.5 3,885,848 - ptsP OKFHMN_20005 0.13 +0.2 3,885,848 - ptsP OKFHMN_20005 0.13 +1.6 3,885,848 - ptsP OKFHMN_20005 0.13 -0.4 3,885,893 + ptsP OKFHMN_20005 0.15 -0.6 3,885,893 + ptsP OKFHMN_20005 0.15 -0.7 3,885,900 + ptsP OKFHMN_20005 0.15 -1.3 3,885,900 + ptsP OKFHMN_20005 0.15 +0.1 3,885,901 - ptsP OKFHMN_20005 0.15 -1.4 3,885,926 + ptsP OKFHMN_20005 0.16 +0.4 3,885,935 + ptsP OKFHMN_20005 0.17 +0.3 3,886,095 - ptsP OKFHMN_20005 0.24 +0.9 3,886,128 - ptsP OKFHMN_20005 0.25 +0.4 3,886,134 - ptsP OKFHMN_20005 0.26 -0.3 3,886,371 - ptsP OKFHMN_20005 0.36 +0.0 3,886,420 - ptsP OKFHMN_20005 0.38 +0.5 3,886,423 + ptsP OKFHMN_20005 0.39 -0.5 3,886,423 + ptsP OKFHMN_20005 0.39 -0.1 3,886,423 + ptsP OKFHMN_20005 0.39 +0.1 3,886,448 + ptsP OKFHMN_20005 0.40 -0.0 3,886,449 - ptsP OKFHMN_20005 0.40 -0.8 3,886,584 - ptsP OKFHMN_20005 0.46 +0.2 3,886,637 + ptsP OKFHMN_20005 0.48 -1.1 3,886,860 - ptsP OKFHMN_20005 0.58 -0.4 3,886,883 + ptsP OKFHMN_20005 0.59 +1.7 3,886,884 - ptsP OKFHMN_20005 0.59 +0.2 3,886,884 - ptsP OKFHMN_20005 0.59 -0.4 3,887,070 + ptsP OKFHMN_20005 0.67 -0.2 3,887,070 + ptsP OKFHMN_20005 0.67 -0.1 3,887,083 + ptsP OKFHMN_20005 0.68 +0.7 3,887,083 + ptsP OKFHMN_20005 0.68 +0.1 3,887,083 + ptsP OKFHMN_20005 0.68 -0.9 3,887,084 - ptsP OKFHMN_20005 0.68 +0.1 3,887,084 - ptsP OKFHMN_20005 0.68 +1.4 3,887,365 - ptsP OKFHMN_20005 0.81 +1.0 3,887,365 - ptsP OKFHMN_20005 0.81 -3.3 3,887,474 - ptsP OKFHMN_20005 0.85 -0.1 3,887,498 + ptsP OKFHMN_20005 0.86 -0.1 3,887,499 - ptsP OKFHMN_20005 0.86 +1.8 3,887,534 + ptsP OKFHMN_20005 0.88 +0.6 3,887,625 + +0.2 3,887,657 - -0.1 3,887,657 - +0.0 3,887,678 - -0.2 3,887,707 - -0.3 3,887,707 - -0.0 3,887,800 + +0.2 3,887,800 + +0.0 3,887,812 + +0.4 3,887,812 + -1.0 3,887,813 - -0.3 3,887,814 + -0.5 3,887,814 + +0.2 3,887,815 - +0.8 3,887,815 - -0.2 3,887,818 + -1.1 3,887,818 + +0.8 3,887,818 + -0.0 3,887,819 - -0.2 3,887,819 - -1.5
Or see this region's nucleotide sequence