Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKFHMN_19815 and OKFHMN_19820 are separated by 13 nucleotides OKFHMN_19820 and sctI are separated by 19 nucleotides sctI and escJ overlap by 4 nucleotides escJ and OKFHMN_19835 are separated by 15 nucleotides OKFHMN_19835 and ygeN overlap by 59 nucleotides ygeN and ygeN are separated by 15 nucleotides
OKFHMN_19815: OKFHMN_19815 - Type III secretion protein EprH, at 3,855,117 to 3,855,851
_19815
OKFHMN_19820: OKFHMN_19820 - type III secretion system needle complex protein, at 3,855,865 to 3,856,104
_19820
OKFHMN_19825: sctI - type III secretion system inner rod subunit SctI, at 3,856,124 to 3,856,456
sctI
OKFHMN_19830: escJ - EscJ/YscJ/HrcJ family type III secretion inner membrane ring protein, at 3,856,453 to 3,857,187
escJ
OKFHMN_19835: OKFHMN_19835 - type III secretion apparatus protein OrgA/MxiK, at 3,857,203 to 3,857,784
_19835
OKFHMN_19840: ygeN - Putative uncharacterized protein YgeN, at 3,857,726 to 3,857,986
ygeN
OKFHMN_19845: ygeN - Putative uncharacterized protein YgeN, at 3,858,002 to 3,858,433
ygeN
Position (kb) 3856
3857
3858 Strain fitness (log2 ratio) -1
0
1
2 at 3855.544 kb on + strand, within OKFHMN_19815 at 3855.571 kb on + strand, within OKFHMN_19815 at 3856.023 kb on + strand, within OKFHMN_19820 at 3856.066 kb on - strand, within OKFHMN_19820 at 3856.138 kb on + strand at 3856.460 kb on - strand at 3856.963 kb on - strand, within escJ at 3857.608 kb on - strand, within OKFHMN_19835 at 3857.719 kb on - strand, within OKFHMN_19835 at 3857.825 kb on - strand, within ygeN at 3858.025 kb on - strand at 3858.142 kb on + strand, within ygeN
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,855,544 + OKFHMN_19815 0.58 -0.4 3,855,571 + OKFHMN_19815 0.62 +0.4 3,856,023 + OKFHMN_19820 0.66 -0.7 3,856,066 - OKFHMN_19820 0.84 -0.6 3,856,138 + -0.1 3,856,460 - -0.4 3,856,963 - escJ OKFHMN_19830 0.69 +2.4 3,857,608 - OKFHMN_19835 0.70 +0.1 3,857,719 - OKFHMN_19835 0.89 +2.4 3,857,825 - ygeN OKFHMN_19840 0.38 +0.1 3,858,025 - +2.1 3,858,142 + ygeN OKFHMN_19845 0.32 +0.9
Or see this region's nucleotide sequence