Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKFHMN_19775 and fliN overlap by 41 nucleotides fliN and escR overlap by 11 nucleotides escR and OKFHMN_19790 are separated by 10 nucleotides OKFHMN_19790 and sctT are separated by 1 nucleotides sctT and sctT are separated by 78 nucleotides
OKFHMN_19775: OKFHMN_19775 - type III secretion protein, at 3,849,216 to 3,849,785
_19775
OKFHMN_19780: fliN - YscQ/HrcQ family type III secretion apparatus protein, at 3,849,745 to 3,850,731
fliN
OKFHMN_19785: escR - EscR/YscR/HrcR family type III secretion system export apparatus protein, at 3,850,721 to 3,851,386
escR
OKFHMN_19790: OKFHMN_19790 - EscS/YscS/HrcS family type III secretion system export apparatus protein, at 3,851,397 to 3,851,657
_19790
OKFHMN_19795: sctT - type III secretion system export apparatus subunit SctT, at 3,851,659 to 3,852,111
sctT
OKFHMN_19800: sctT - type III secretion system export apparatus subunit SctT, at 3,852,190 to 3,852,426
sctT
Position (kb)
3850
3851
3852 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3849.875 kb on + strand, within fliN at 3850.408 kb on + strand, within fliN at 3851.217 kb on + strand, within escR at 3851.258 kb on - strand, within escR at 3851.287 kb on + strand, within escR at 3851.672 kb on + strand at 3851.801 kb on + strand, within sctT at 3851.919 kb on - strand, within sctT at 3852.271 kb on + strand, within sctT
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,849,875 + fliN OKFHMN_19780 0.13 -0.3 3,850,408 + fliN OKFHMN_19780 0.67 +0.0 3,851,217 + escR OKFHMN_19785 0.74 -2.7 3,851,258 - escR OKFHMN_19785 0.81 -1.1 3,851,287 + escR OKFHMN_19785 0.85 -0.3 3,851,672 + +0.0 3,851,801 + sctT OKFHMN_19795 0.31 -0.3 3,851,919 - sctT OKFHMN_19795 0.57 +1.9 3,852,271 + sctT OKFHMN_19800 0.34 +1.2
Or see this region's nucleotide sequence