Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hybC and hybD overlap by 1 nucleotides hybD and hybE overlap by 8 nucleotides hybE and hypA overlap by 8 nucleotides hypA and hybG are separated by 12 nucleotides hybG and yghU are separated by 122 nucleotides
OKFHMN_19020: hybC - hydrogenase 2 large subunit, at 3,693,053 to 3,694,756
hybC
OKFHMN_19025: hybD - HyaD/HybD family hydrogenase maturation endopeptidase, at 3,694,756 to 3,695,250
hybD
OKFHMN_19030: hybE - hydrogenase-2 assembly chaperone, at 3,695,243 to 3,695,731
hybE
OKFHMN_19035: hypA - hydrogenase maturation nickel metallochaperone HypA, at 3,695,724 to 3,696,065
hypA
OKFHMN_19040: hybG - hydrogenase maturation factor HybG, at 3,696,078 to 3,696,326
hybG
OKFHMN_19050: yghU - glutathione-dependent disulfide-bond oxidoreductase, at 3,696,449 to 3,697,315
yghU
Position (kb) 3695
3696 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3694.379 kb on + strand, within hybC at 3694.492 kb on + strand, within hybC at 3694.539 kb on - strand, within hybC at 3694.539 kb on - strand, within hybC at 3694.539 kb on - strand, within hybC at 3694.539 kb on - strand, within hybC at 3694.539 kb on - strand, within hybC at 3694.579 kb on + strand, within hybC at 3694.625 kb on - strand at 3694.646 kb on - strand at 3694.666 kb on + strand at 3694.667 kb on - strand at 3694.667 kb on - strand at 3694.755 kb on - strand at 3694.761 kb on + strand at 3694.858 kb on + strand, within hybD at 3694.858 kb on + strand, within hybD at 3694.859 kb on - strand, within hybD at 3695.257 kb on - strand at 3695.289 kb on + strand at 3695.289 kb on + strand at 3695.555 kb on + strand, within hybE at 3695.556 kb on - strand, within hybE at 3695.908 kb on + strand, within hypA at 3695.909 kb on - strand, within hypA at 3695.912 kb on + strand, within hypA at 3695.912 kb on + strand, within hypA at 3695.919 kb on + strand, within hypA at 3695.921 kb on + strand, within hypA at 3695.922 kb on - strand, within hypA at 3696.074 kb on - strand at 3696.083 kb on + strand at 3696.148 kb on + strand, within hybG at 3696.148 kb on + strand, within hybG at 3696.148 kb on + strand, within hybG at 3696.148 kb on + strand, within hybG at 3696.149 kb on - strand, within hybG at 3696.194 kb on + strand, within hybG at 3696.194 kb on + strand, within hybG at 3696.283 kb on + strand, within hybG at 3696.283 kb on + strand, within hybG at 3696.315 kb on - strand at 3696.341 kb on - strand at 3696.463 kb on + strand at 3696.609 kb on + strand, within yghU at 3696.609 kb on + strand, within yghU at 3696.609 kb on + strand, within yghU at 3696.610 kb on - strand, within yghU at 3696.702 kb on + strand, within yghU at 3696.703 kb on - strand, within yghU at 3696.724 kb on + strand, within yghU
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,694,379 + hybC OKFHMN_19020 0.78 +0.6 3,694,492 + hybC OKFHMN_19020 0.84 +0.2 3,694,539 - hybC OKFHMN_19020 0.87 +0.2 3,694,539 - hybC OKFHMN_19020 0.87 +1.1 3,694,539 - hybC OKFHMN_19020 0.87 -0.1 3,694,539 - hybC OKFHMN_19020 0.87 -0.1 3,694,539 - hybC OKFHMN_19020 0.87 -0.1 3,694,579 + hybC OKFHMN_19020 0.90 -0.0 3,694,625 - +2.0 3,694,646 - +0.4 3,694,666 + -0.7 3,694,667 - -0.2 3,694,667 - +0.6 3,694,755 - +0.2 3,694,761 + +0.5 3,694,858 + hybD OKFHMN_19025 0.21 +0.1 3,694,858 + hybD OKFHMN_19025 0.21 +0.5 3,694,859 - hybD OKFHMN_19025 0.21 +1.4 3,695,257 - -0.0 3,695,289 + -0.9 3,695,289 + +0.2 3,695,555 + hybE OKFHMN_19030 0.64 +0.2 3,695,556 - hybE OKFHMN_19030 0.64 -3.0 3,695,908 + hypA OKFHMN_19035 0.54 +0.0 3,695,909 - hypA OKFHMN_19035 0.54 -0.5 3,695,912 + hypA OKFHMN_19035 0.55 +1.3 3,695,912 + hypA OKFHMN_19035 0.55 -0.7 3,695,919 + hypA OKFHMN_19035 0.57 -0.4 3,695,921 + hypA OKFHMN_19035 0.58 -2.0 3,695,922 - hypA OKFHMN_19035 0.58 +0.3 3,696,074 - +0.1 3,696,083 + -0.1 3,696,148 + hybG OKFHMN_19040 0.28 -1.4 3,696,148 + hybG OKFHMN_19040 0.28 -0.6 3,696,148 + hybG OKFHMN_19040 0.28 +0.4 3,696,148 + hybG OKFHMN_19040 0.28 +0.1 3,696,149 - hybG OKFHMN_19040 0.29 +0.6 3,696,194 + hybG OKFHMN_19040 0.47 -0.1 3,696,194 + hybG OKFHMN_19040 0.47 +0.4 3,696,283 + hybG OKFHMN_19040 0.82 -0.0 3,696,283 + hybG OKFHMN_19040 0.82 -0.7 3,696,315 - +0.8 3,696,341 - -0.5 3,696,463 + +1.4 3,696,609 + yghU OKFHMN_19050 0.18 +0.4 3,696,609 + yghU OKFHMN_19050 0.18 -0.6 3,696,609 + yghU OKFHMN_19050 0.18 +0.9 3,696,610 - yghU OKFHMN_19050 0.19 +0.5 3,696,702 + yghU OKFHMN_19050 0.29 -0.1 3,696,703 - yghU OKFHMN_19050 0.29 +0.3 3,696,724 + yghU OKFHMN_19050 0.32 -1.0
Or see this region's nucleotide sequence