Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frlB and frlA are separated by 20 nucleotides frlA and yhfL are separated by 295 nucleotides yhfL and cysG are separated by 254 nucleotides
OKFHMN_17165: frlB - fructoselysine 6-phosphate deglycase, at 3,352,876 to 3,353,898
frlB
OKFHMN_17170: frlA - fructoselysine/psicoselysine transporter, at 3,353,919 to 3,355,256
frlA
OKFHMN_17175: yhfL - Uncharacterized protein YhfL, at 3,355,552 to 3,355,719
yhfL
OKFHMN_17180: cysG - siroheme synthase CysG, at 3,355,974 to 3,357,347
cysG
Position (kb)
3353
3354
3355
3356 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3352.931 kb on + strand at 3352.932 kb on - strand at 3352.932 kb on - strand at 3352.935 kb on + strand at 3352.936 kb on - strand at 3352.943 kb on + strand at 3352.944 kb on - strand at 3352.944 kb on - strand at 3352.944 kb on - strand at 3352.946 kb on + strand at 3353.021 kb on + strand, within frlB at 3353.046 kb on + strand, within frlB at 3353.047 kb on - strand, within frlB at 3353.047 kb on - strand, within frlB at 3353.065 kb on - strand, within frlB at 3353.065 kb on - strand, within frlB at 3353.471 kb on + strand, within frlB at 3353.481 kb on + strand, within frlB at 3353.576 kb on - strand, within frlB at 3353.590 kb on - strand, within frlB at 3353.676 kb on - strand, within frlB at 3353.756 kb on + strand, within frlB at 3353.851 kb on - strand at 3353.901 kb on + strand at 3353.902 kb on - strand at 3353.902 kb on - strand at 3353.914 kb on + strand at 3353.921 kb on - strand at 3353.966 kb on - strand at 3354.177 kb on + strand, within frlA at 3354.178 kb on - strand, within frlA at 3354.190 kb on + strand, within frlA at 3354.353 kb on - strand, within frlA at 3354.530 kb on - strand, within frlA at 3354.608 kb on + strand, within frlA at 3354.609 kb on - strand, within frlA at 3354.609 kb on - strand, within frlA at 3354.609 kb on - strand, within frlA at 3354.609 kb on - strand, within frlA at 3354.610 kb on + strand, within frlA at 3354.610 kb on + strand, within frlA at 3354.617 kb on + strand, within frlA at 3354.617 kb on + strand, within frlA at 3354.618 kb on - strand, within frlA at 3354.618 kb on - strand, within frlA at 3354.789 kb on - strand, within frlA at 3354.843 kb on - strand, within frlA at 3354.856 kb on - strand, within frlA at 3354.856 kb on - strand, within frlA at 3354.856 kb on - strand, within frlA at 3355.059 kb on + strand, within frlA at 3355.069 kb on + strand, within frlA at 3355.070 kb on - strand, within frlA at 3355.071 kb on + strand, within frlA at 3355.071 kb on + strand, within frlA at 3355.072 kb on - strand, within frlA at 3355.072 kb on - strand, within frlA at 3355.287 kb on + strand at 3355.309 kb on + strand at 3355.348 kb on + strand at 3355.380 kb on + strand at 3355.476 kb on - strand at 3355.476 kb on - strand at 3355.476 kb on - strand at 3355.476 kb on - strand at 3355.485 kb on + strand at 3355.486 kb on - strand at 3355.531 kb on + strand at 3355.563 kb on + strand at 3355.616 kb on + strand, within yhfL at 3355.622 kb on + strand, within yhfL at 3355.623 kb on - strand, within yhfL at 3355.635 kb on + strand, within yhfL at 3355.650 kb on + strand, within yhfL at 3355.650 kb on + strand, within yhfL at 3355.659 kb on + strand, within yhfL at 3355.660 kb on - strand, within yhfL at 3355.741 kb on + strand at 3355.922 kb on + strand at 3355.922 kb on + strand at 3356.204 kb on + strand, within cysG at 3356.205 kb on - strand, within cysG at 3356.206 kb on + strand, within cysG at 3356.206 kb on + strand, within cysG at 3356.207 kb on - strand, within cysG at 3356.207 kb on - strand, within cysG at 3356.207 kb on - strand, within cysG at 3356.207 kb on - strand, within cysG at 3356.207 kb on - strand, within cysG at 3356.207 kb on - strand, within cysG
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,352,931 + +0.3 3,352,932 - +0.0 3,352,932 - -0.1 3,352,935 + -0.6 3,352,936 - +1.1 3,352,943 + -0.5 3,352,944 - -0.7 3,352,944 - +0.0 3,352,944 - +0.1 3,352,946 + +0.3 3,353,021 + frlB OKFHMN_17165 0.14 +0.3 3,353,046 + frlB OKFHMN_17165 0.17 -0.8 3,353,047 - frlB OKFHMN_17165 0.17 -0.1 3,353,047 - frlB OKFHMN_17165 0.17 -0.5 3,353,065 - frlB OKFHMN_17165 0.18 +0.5 3,353,065 - frlB OKFHMN_17165 0.18 +0.1 3,353,471 + frlB OKFHMN_17165 0.58 +0.4 3,353,481 + frlB OKFHMN_17165 0.59 +0.1 3,353,576 - frlB OKFHMN_17165 0.68 +0.4 3,353,590 - frlB OKFHMN_17165 0.70 +0.3 3,353,676 - frlB OKFHMN_17165 0.78 +0.2 3,353,756 + frlB OKFHMN_17165 0.86 -0.0 3,353,851 - -0.5 3,353,901 + -2.6 3,353,902 - +0.3 3,353,902 - +1.5 3,353,914 + +1.4 3,353,921 - -0.2 3,353,966 - +0.2 3,354,177 + frlA OKFHMN_17170 0.19 +0.2 3,354,178 - frlA OKFHMN_17170 0.19 -0.3 3,354,190 + frlA OKFHMN_17170 0.20 +0.5 3,354,353 - frlA OKFHMN_17170 0.32 -0.2 3,354,530 - frlA OKFHMN_17170 0.46 -0.6 3,354,608 + frlA OKFHMN_17170 0.51 -1.1 3,354,609 - frlA OKFHMN_17170 0.52 +1.1 3,354,609 - frlA OKFHMN_17170 0.52 +0.4 3,354,609 - frlA OKFHMN_17170 0.52 -0.3 3,354,609 - frlA OKFHMN_17170 0.52 +0.3 3,354,610 + frlA OKFHMN_17170 0.52 -0.3 3,354,610 + frlA OKFHMN_17170 0.52 +0.6 3,354,617 + frlA OKFHMN_17170 0.52 -0.2 3,354,617 + frlA OKFHMN_17170 0.52 +0.7 3,354,618 - frlA OKFHMN_17170 0.52 +0.1 3,354,618 - frlA OKFHMN_17170 0.52 -0.3 3,354,789 - frlA OKFHMN_17170 0.65 -0.2 3,354,843 - frlA OKFHMN_17170 0.69 -0.5 3,354,856 - frlA OKFHMN_17170 0.70 +0.2 3,354,856 - frlA OKFHMN_17170 0.70 -0.0 3,354,856 - frlA OKFHMN_17170 0.70 +0.6 3,355,059 + frlA OKFHMN_17170 0.85 -1.5 3,355,069 + frlA OKFHMN_17170 0.86 -0.1 3,355,070 - frlA OKFHMN_17170 0.86 +0.9 3,355,071 + frlA OKFHMN_17170 0.86 +1.0 3,355,071 + frlA OKFHMN_17170 0.86 -0.7 3,355,072 - frlA OKFHMN_17170 0.86 +0.0 3,355,072 - frlA OKFHMN_17170 0.86 -1.4 3,355,287 + +0.2 3,355,309 + -3.1 3,355,348 + +0.9 3,355,380 + +0.5 3,355,476 - +0.1 3,355,476 - +1.2 3,355,476 - +0.7 3,355,476 - +1.1 3,355,485 + -1.7 3,355,486 - +1.5 3,355,531 + -1.2 3,355,563 + -0.9 3,355,616 + yhfL OKFHMN_17175 0.38 +0.1 3,355,622 + yhfL OKFHMN_17175 0.42 -0.3 3,355,623 - yhfL OKFHMN_17175 0.42 -1.8 3,355,635 + yhfL OKFHMN_17175 0.49 -0.2 3,355,650 + yhfL OKFHMN_17175 0.58 +1.0 3,355,650 + yhfL OKFHMN_17175 0.58 -0.1 3,355,659 + yhfL OKFHMN_17175 0.64 -1.3 3,355,660 - yhfL OKFHMN_17175 0.64 -0.2 3,355,741 + -1.0 3,355,922 + -0.2 3,355,922 + +1.0 3,356,204 + cysG OKFHMN_17180 0.17 +0.4 3,356,205 - cysG OKFHMN_17180 0.17 +0.6 3,356,206 + cysG OKFHMN_17180 0.17 +0.6 3,356,206 + cysG OKFHMN_17180 0.17 +0.4 3,356,207 - cysG OKFHMN_17180 0.17 -0.2 3,356,207 - cysG OKFHMN_17180 0.17 +0.2 3,356,207 - cysG OKFHMN_17180 0.17 -0.3 3,356,207 - cysG OKFHMN_17180 0.17 -0.7 3,356,207 - cysG OKFHMN_17180 0.17 +0.5 3,356,207 - cysG OKFHMN_17180 0.17 -0.4
Or see this region's nucleotide sequence