Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dam and dam are separated by 17 nucleotides dam and gph overlap by 8 nucleotides gph and trpS overlap by 8 nucleotides
OKFHMN_17125: dam - adenine-specific DNA-methyltransferase, at 3,346,972 to 3,347,808
dam
OKFHMN_17130: dam - adenine-specific DNA-methyltransferase, at 3,347,826 to 3,348,503
dam
OKFHMN_17135: gph - phosphoglycolate phosphatase, at 3,348,496 to 3,349,254
gph
OKFHMN_17140: trpS - tryptophan--tRNA ligase, at 3,349,247 to 3,350,251
trpS
Position (kb)
3348
3349
3350 Strain fitness (log2 ratio)
-2
-1
0
1 at 3348.009 kb on - strand, within dam at 3348.490 kb on + strand at 3348.491 kb on - strand at 3348.501 kb on + strand at 3348.501 kb on + strand at 3348.502 kb on - strand at 3348.513 kb on + strand at 3348.582 kb on - strand, within gph at 3348.582 kb on - strand, within gph at 3348.582 kb on - strand, within gph at 3348.582 kb on - strand, within gph at 3348.726 kb on + strand, within gph at 3348.767 kb on + strand, within gph at 3348.767 kb on + strand, within gph at 3348.767 kb on + strand, within gph at 3348.771 kb on + strand, within gph at 3348.771 kb on + strand, within gph at 3348.771 kb on + strand, within gph at 3348.771 kb on + strand, within gph at 3348.772 kb on - strand, within gph at 3348.772 kb on - strand, within gph at 3348.781 kb on - strand, within gph at 3348.796 kb on + strand, within gph at 3348.796 kb on + strand, within gph at 3348.796 kb on + strand, within gph at 3348.797 kb on - strand, within gph at 3348.797 kb on - strand, within gph at 3348.800 kb on - strand, within gph at 3348.850 kb on - strand, within gph at 3348.850 kb on - strand, within gph at 3348.938 kb on + strand, within gph at 3348.938 kb on + strand, within gph at 3348.938 kb on + strand, within gph at 3348.939 kb on - strand, within gph at 3349.129 kb on + strand, within gph at 3349.130 kb on - strand, within gph at 3349.135 kb on + strand, within gph at 3349.137 kb on + strand, within gph at 3349.138 kb on - strand, within gph at 3349.138 kb on - strand, within gph at 3349.138 kb on - strand, within gph at 3349.141 kb on + strand, within gph at 3349.175 kb on + strand, within gph at 3349.180 kb on + strand at 3349.180 kb on + strand at 3349.188 kb on + strand at 3349.190 kb on + strand at 3349.190 kb on + strand at 3350.249 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,348,009 - dam OKFHMN_17130 0.27 -0.3 3,348,490 + +0.2 3,348,491 - +0.1 3,348,501 + +0.4 3,348,501 + -0.4 3,348,502 - -1.7 3,348,513 + -0.3 3,348,582 - gph OKFHMN_17135 0.11 +0.6 3,348,582 - gph OKFHMN_17135 0.11 +0.1 3,348,582 - gph OKFHMN_17135 0.11 -1.0 3,348,582 - gph OKFHMN_17135 0.11 -0.7 3,348,726 + gph OKFHMN_17135 0.30 +0.7 3,348,767 + gph OKFHMN_17135 0.36 -0.2 3,348,767 + gph OKFHMN_17135 0.36 -0.0 3,348,767 + gph OKFHMN_17135 0.36 +0.3 3,348,771 + gph OKFHMN_17135 0.36 -0.4 3,348,771 + gph OKFHMN_17135 0.36 +0.2 3,348,771 + gph OKFHMN_17135 0.36 +0.1 3,348,771 + gph OKFHMN_17135 0.36 +0.5 3,348,772 - gph OKFHMN_17135 0.36 +0.7 3,348,772 - gph OKFHMN_17135 0.36 +0.4 3,348,781 - gph OKFHMN_17135 0.38 +1.1 3,348,796 + gph OKFHMN_17135 0.40 +0.3 3,348,796 + gph OKFHMN_17135 0.40 -0.3 3,348,796 + gph OKFHMN_17135 0.40 -0.5 3,348,797 - gph OKFHMN_17135 0.40 +0.7 3,348,797 - gph OKFHMN_17135 0.40 -0.7 3,348,800 - gph OKFHMN_17135 0.40 -0.0 3,348,850 - gph OKFHMN_17135 0.47 -0.3 3,348,850 - gph OKFHMN_17135 0.47 -0.1 3,348,938 + gph OKFHMN_17135 0.58 -0.1 3,348,938 + gph OKFHMN_17135 0.58 +0.1 3,348,938 + gph OKFHMN_17135 0.58 -0.4 3,348,939 - gph OKFHMN_17135 0.58 -0.1 3,349,129 + gph OKFHMN_17135 0.83 -2.0 3,349,130 - gph OKFHMN_17135 0.84 +0.3 3,349,135 + gph OKFHMN_17135 0.84 -0.2 3,349,137 + gph OKFHMN_17135 0.84 +0.0 3,349,138 - gph OKFHMN_17135 0.85 -0.3 3,349,138 - gph OKFHMN_17135 0.85 -0.3 3,349,138 - gph OKFHMN_17135 0.85 -0.5 3,349,141 + gph OKFHMN_17135 0.85 -2.1 3,349,175 + gph OKFHMN_17135 0.89 -1.0 3,349,180 + -1.0 3,349,180 + -0.5 3,349,188 + -0.9 3,349,190 + +0.2 3,349,190 + -0.1 3,350,249 + +0.9
Or see this region's nucleotide sequence